Translocation analysis by the FISH-painting method for retrospective dose reconstruction in individuals exposed to ionizing radiation 10 years after exposure

Camparoto, Marjori Leiva; Ramalho, A.T.; Natarajan, A.T.; Curado, María Paula; Sakamoto-Hojo, Elza T.

doi:10.1016/s0027-5107(03)00132-5

Cited by 43 publications

(29 citation statements)

References 26 publications

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“…Biological dosimetry using the analysis of unstable chromosomal aberrations in human lymphocytes from peripheral blood is well established and accurate, especially in the case of acute exposure, and when the blood samples are obtained within few days after the real or suspected radiation exposure (Lucas, 1997;IAEA, 2001;Camparoto et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation

et al. 2005

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Scoring of unstable chromosome aberrations (dicentrics, rings and fragments) and micronuclei in circulating lymphocytes are the most extensively studied biological means for estimating individual exposure to ionizing radiation (IR), which can be used as complementary methods to physical dosimetry or when the latter cannot be performed. In this work, the quantification of the frequencies of chromosome aberrations and micronuclei were carried out based on cytogenetic analyses of peripheral blood samples from 5 patients with cervical uterine cancer following radiotherapy in order to evaluate the absorbed dose as a result of partial-body exposure to 60 Co source. Blood samples were collected from each patient in three phases of the treatment: before irradiation, 24 h after receiving 0.08 Gy and 1.8 Gy, respectively. The results presented in this report emphasize biological dosimetry, employing the quantification of chromosome aberrations and micronuclei in lymphocytes from peripheral blood, as an important methodology of dose assessment for either whole or partial-body exposure to IR.

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Section: Introductionmentioning

confidence: 99%

Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation

et al. 2005

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“…For example, it may be possible to discern 90 Sr exposure using a photostimulable phosphor imaging detector (Romanyukha et al, 2002b) and dose to bone marrow from internal plutonium exposure using mFISH (Hande et al, 2003) has been demonstrated. In addition, FISH has been shown to work well for a limited number of incorporated radionuclides e.g., for 137 Cs, where it was used following the Goiania accident (Camparoto et al, 2003) and for tritiated water, where it was used 6 and 11 years after an industrial accident (Lloyd et al, 1998). In both of those cases, the body received generally uniform exposure due to the tendency of those specific nuclides to distribute themselves uniformly.…”

Section: Discussionmentioning

confidence: 99%

“…Among these are the Japanese A-bomb survivors ), Chernobyl liquidators (Salassidis et al, 1994;Moore et al, 1997;Littlefield et al, 1998;Jones et al, 2002), Sellafield British Nuclear Fuels workers (Tucker et al, 1997a, b;Tawn et al, 2000Tawn et al, , 2004, Mayak nuclear workers Bauchinger et al, 2001;Burak et al, 2001), residents living near Techa River and the Semipalatinsk test sites (Stephan et al, 2001;Salomaa et al, 2002), residents of buildings contaminated with 60 Co in Taiwan (Chen et al, 2000), radiation accident victims in Goiania (Straume et al, 1991;Natarajan et al, 1998;Camparoto et al, 2003), astronauts (George et al, 2005) Several of these investigations are worth highlighting. In the Sellafield workers, it was shown that chronic exposures produced approximately six-fold fewer chromosome aberrations per unit dose compared to the acute exposures received by the A-bomb survivors (Tucker et al, 1997a, b).…”

Section: Use Of Fish In Epidemiologic Studies Following Whole-body Exmentioning

confidence: 99%

BiodosEPR-2006 consensus committee report on biodosimetric methods to evaluate radiation doses at long times after exposure

Simon

Bailiff

Bouville

et al. 2007

Radiation Measurements

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'kaev, Alexander V.; Tucker, James D.; and Wieser, Albrecht, "BiodosEPR-2006 consensus committee report on biodosimetric methods to evaluate radiation doses at long times after exposure" (2007 AbstractThe requirements for biodosimetric techniques used at long times after exposure, i.e., 6 months to more than 50 years, are unique compared to the requirements for methods used for immediate dose estimation. In addition to the fundamental requirement that the assay measures a physical or biologic change that is proportional to the energy absorbed, the signal must be highly stable over time to enable reasonably precise determinations of the absorbed dose decades later. The primary uses of these biodosimetric methods have been to support long-term health risk (epidemiologic) studies or to support compensation (damage) claims. For these reasons, the methods must be capable of estimating individual doses, rather than group mean doses. Even when individual dose estimates can be obtained, inter-individual variability remains as one of the most difficult problems in using biodosimetry measurements to rigorously quantify individual exposures. Other important criteria for biodosimetry methods include obtaining samples with minimal invasiveness, low detection limits, and high precision. Cost and other practical limitations generally prohibit biodosimetry measurements on a large enough sample to replace analytical dose reconstruction in epidemiologic investigations. However, these measurements can be extremely valuable as a means to corroborate analytical or model-based dose estimates, to help reduce uncertainty in individual doses estimated by other methods and techniques, and to assess bias in dose reconstruction models. There has been extensive use of three biodosimetric techniques in irradiated populations: EPR (using tooth enamel), FISH (using blood lymphocytes), and GPA (also using blood); these methods have been supplemented with luminescent methods applied to building materials and artifacts. A large number of investigations have used biodosimetric methods many years after external and, to a lesser extent, internal exposure to reconstruct doses received from accidents, from occupational exposures, from environmental releases of radioactive materials, and from medical exposures. In most applications, the intent has been to either identify highly exposed persons or confirmed suspected exposures. Improvements in methodology, however, have led many investigators to attempt quantification of whole-body doses received, or in a few instances, to estimate organ doses. There will be a continued need for new and improved biodosimetric techniques not only to assist in future epidemiologic investigations but to help evaluate the long-term consequences following nuclear accidents or events of radiologic terrorism.

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“…The Goiania radiation accident: A good cohort for such a study is the victims of the radiation accident in Goiania (Brazil) [17,34,35]. In this accident which occurred in September 1987, a cesium-137 ( 137 Cs) therapy unit was broken, and the radioactive 137 Cs was distributed, as a result of which 249 individuals were contaminated either internally or externally.…”

Section: Comparison To Non-chernobyl Studiesmentioning

confidence: 99%

“…In this accident the exposure was complicated (fractionated, protracted and internal) and resulted in most cases in a distribution that was not distinguishable from Poisson. The victims of Goiania radiation accident were analyzed immediately and followed for 10 years [17,35]. The frequencies of dicentrics were determined immediately following exposure in 1987 and onwards, and translocations were determined since 1992.…”

Section: Comparison To Non-chernobyl Studiesmentioning

confidence: 99%

Cytogenetic Biological Dosimetry Past, Present and Future Perspectives

Darroudi

2012

Sixth International Conference on Environmental Mutagens in Human Populations

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Abstract. Human risk assessments at the low doses, and low dose rates and high doses following acute exposure to ionizing radiation are of prime importance in radiation protection. These issues are of continuing importance in respect of social/economic policy relating to the industrial and medical uses of ionizing radiation, and for risk assessment among people occupationally are being exposed to low and/or high LET radiation, such as astronauts, pilots, stewardess and nuclear power plant workers, as well as victims of radiation accidents. Consequently, several biological assays were developed and attempts were made to investigate formation of radiation induced chromosome aberrations and induction of genomic instability in human lymphocytes and fibroblasts. Fluorescence in situ hybridization (FISH) technique using chromosome, chromosome-arm, chromosome region, centromere and telomere specific DNA libraries has improved the resolution of detecting all classes of radiation induced chromosomal inter-and intra-changes. Consequently, has increased significantly the accuracy and detection limit of biological dosimetry. Newly obtained data indicate that (a) Premature chromosome condensation assay is a unique method to be used for immediate dose assessment at low (5cGy) as well as high doses (≥3 Gy) and can accurately discriminate between whole-and partial-body exposure in case of mass casualties and accidental over-exposure to high doses of ionizing radiation (b) FISH-based translocation assay has the potential to assess acute as well as chronic exposure in cases of accidental as well as occupational exposure to ionizing radiation, either immediately following exposure, or retrospectively by defining accumulative effects to red bone marrows. (c) there are distinct finger-prints (such as insertions and complex translocations) for high LET radiation in comparison to low LET radiation. The importance of these findings and future perspectives for biological dosimetry are discussed.

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Translocation analysis by the FISH-painting method for retrospective dose reconstruction in individuals exposed to ionizing radiation 10 years after exposure

Cited by 43 publications

References 26 publications

Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation

Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation

BiodosEPR-2006 consensus committee report on biodosimetric methods to evaluate radiation doses at long times after exposure

Cytogenetic Biological Dosimetry Past, Present and Future Perspectives

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