SummaryA description of a cultivation column and an adequate cultivation technique to be used in studying transmigration cultures in Sephadex G-200 environment is presented. The cultivation column is tubular with two adjustable plungers the lower of which is covered by a polypropylene filter. Inoculation of the cultivation column is done from below and the liquid nutrient for the upper part of the column is fed i n by means of gas from an electrolyzer.