Transmission electron microscopy analysis of hydroxyapatite nanocrystals from cattle bones

Patel, S. M.; Wei, Shanghai; Han, Jie; Gao, Wei

doi:10.1016/j.matchar.2015.09.019

Cited by 26 publications

(13 citation statements)

References 14 publications

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“…While bioapatite crystals tend to have a more elongated and sometimes needle-like shape16181920212426, apatite crystals from fossil bones typically have platelet-like shapes with a greater short-axis length5678 which is also shown in our study (Supplementary Fig. S5).…”

Section: Resultssupporting

confidence: 80%

“…S5). It has been demonstrated that heat treatment of bioapatite causes an increase in apatite crystallinity26 and along with pressure and the influx and interaction of elements from the depositional environment, diagenetic alteration of apatite occurs46474849. The increased crystallinity and dimension of diagenetically altered apatite in fossils may directly contribute to the preservation of fossil bone nanostructure as the open spaces left by decomposed collagen become reduced4849.…”

Section: Resultsmentioning

confidence: 99%

“…While a method of investigating extant bone nanostructures in three dimensions using small-angle scattering tensor tomography has been recently developed13, in fossilized bones, several mineral phases originating from the depositional environment and its effects must be taken into account571415. The continuous advances in EM combined with a variety of analytical techniques have greatly enhanced the understanding of general bone nanostructure and chemistry161718192021222324252627. Such studies applied in fossils have revealed the supposed identity of organic phases in 75-million-year-old dinosaur bones2829.…”

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confidence: 99%

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Disparities in correlating microstructural to nanostructural preservation of dinosaur femoral bones

Kim

Kwon

Lee

et al. 2017

Sci Rep

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Osteohistological researches on dinosaurs are well documented, but descriptions of direct correlations between the bone microstructure and corresponding nanostructure are currently lacking. By applying correlative microscopy, we aimed to verify that well-preserved osteohistological features correlate with pristine fossil bone nanostructures from the femoral bones of Koreanosaurus boseongensis. The quality of nanostructural preservation was evaluated based on the preferred orientation level of apatite crystals obtained from selected area electron diffraction (SAED) patterns and by measuring the “arcs” from the {100} and {002} diffraction rings. Unlike our expectations, our results revealed that well-preserved microstructures do not guarantee pristine nanostructures and vice versa. Structural preservation of bone from macro- to nanoscale primarily depends on original bioapatite density, and subsequent taphonomical factors such as effects from burial, pressure, influx of external elements and the rate of diagenetic alteration of apatite crystals. Our findings suggest that the efficient application of SAED analysis opens the opportunity for comprehensive nanostructural investigations of bone.

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Disparities in correlating microstructural to nanostructural preservation of dinosaur femoral bones

Kim

Kwon

Lee

et al. 2017

Sci Rep

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“…TEM analyses showed the presence of well‐defined nanocrystals and the (112) and (002) planes for bovine bone, (211) for porcine, and (112) for human, thus indicating a high crystalline order. Patel et al pointed out that raw bone powder from bovine is crystalline based on their TEM images. As was mentioned before, the nanosized dimensions of the HAp crystals from all these bones have a strong influence on the vibrational properties of bone.…”

Section: Discussionmentioning

confidence: 99%

“…In these cases, the elastic and inelastic signals are detected at the same time by the X‐Ray detector, affecting the peak width and shape of the patterns. Patel et al using transmission electron microscopy (TEM) images reported a nanometric size for the bovine bone, but they pointed out that this sample was amorphous. Nonetheless, a detailed analysis of their HRTEM images showed these to correspond to ordered crystals.…”

Section: Introductionmentioning

confidence: 99%

Effect of the crystal size on the infrared and Raman spectra of bio hydroxyapatite of human, bovine, and porcine bones

Londoño‐Restrepo

Zubieta‐Otero

Jeronimo‐Cruz

et al. 2019

J Raman Spectroscopy

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The optical properties of hydroxyapatites (HAps) and bio HAps have been studied using Raman and infrared (IR) spectroscopies to describe their crystalline quality. However, the size of the HAp crystals and their crystalline order effects have not been considered yet. This paper focuses on the study of the effect of the change in the crystallites size have on the width of the IR and Raman spectra for defatted and deproteinized bones as well as incinerated biogenic HAp obtained from bovine, porcine, and human bones. Bone samples were analyzed through Raman and IR spectroscopies, X‐ray diffraction (XRD), transmission electron microscopy (TEM), inductively coupled plasma (ICP), and scanning electron microscopy (SEM). The Raman and IR spectra for raw samples showed broad bands but, after calcination at 720 °C, became narrow and well defined. TEM images showed that all raw crystallites are ordered nanoplates contrary to the so far well‐established concept that biogenic HAps have low crystalline quality. XRD data confirmed that raw samples display broad peaks that correlated with the HRTEM images of ordered nanocrystals. This fact confirmed that the broad Raman and IR bands of raw clean bones come from nanocrystal plates. SEM analysis confirmed the increase in the crystal size after calcination from nanomicron to submicron dimensions due to a coalescence phenomenon. These results imply that the interpretation of Raman and IR spectra in the case of HAp nanoparticles has been erroneous. These results contribute to the design of biomaterials for tissue engineering based on biogenic HAp for bone regeneration.

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Bio‐inspired synthesis of bio‐hydroxyapatite/synthetic hydroxyapatite hybrid nanosystems

Zubieta‐Otero,

Gomez‐Vazquez,

Correa‐Piña

et al. 2023

MedComm – Biomaterials and Applications

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The current study focuses on the synthesizing of novel hybrid nanosystems of bio‐hydroxyapatite (BioHAp)/synthetic‐hydroxyapatite (HAp‐Syn). Bio‐HAp was defatted, deproteinized, and then sonicated to obtain nanoparticles (nBioHAp). Hybrid nanosystems (HNS) were synthesized by wet chemical precipitation using nBioHAp as nucleation sites and applying four different precipitation times of 45, 90, 135, and 180 min. X‐ray diffraction (XRD) showed that the patterns of the nBioHAp and HAp‐Syn samples have broadened diffraction peaks due to simultaneous elastic and inelastic scattering. Their crystallite size is about 25 nm, while the hybrid nanosystems have a size between 35 and 40 nm. Fourier‐transform infrared spectroscopy (FT‐IR) revealed the main groups associated with hydroxyapatite (PO32−, OH−, and CO32−), and the nanometer character of the crystals was demonstrated by studying the full width at half maximum (FWHM) of the spectra. Scanning electron microscopy (SEM) revealed the differences between the morphologies of nBioHAp and HAp‐Syn. The shape of HNS resembled that of biogenic HAp, which was confirmed by transmission electron microscopy (TEM). Inductively coupled plasma spectroscopy (ICP‐OES) and energy dispersive X‐ray spectroscopy (EDS) were used to confirm the elemental composition of Mg, Na, K, and Zn as minority ions.

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Transmission electron microscopy analysis of hydroxyapatite nanocrystals from cattle bones

Cited by 26 publications

References 14 publications

Disparities in correlating microstructural to nanostructural preservation of dinosaur femoral bones

Disparities in correlating microstructural to nanostructural preservation of dinosaur femoral bones

Effect of the crystal size on the infrared and Raman spectra of bio hydroxyapatite of human, bovine, and porcine bones

Bio‐inspired synthesis of bio‐hydroxyapatite/synthetic hydroxyapatite hybrid nanosystems

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