Transmission of Cotton Seed and Boll Rotting Bacteria by the Verde Plant Bug (Hemiptera: Miridae)

Glover, James P.; Medrano, Enrique G.; Isakeit, Thomas; Brewer, Michael J.

doi:10.1093/jee/toz334

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…Drosophila melanogaster has been reported to be an important media for Erwinia carotovora ( Vieira et al, 2020 ). The Hemiptera insect Creontiades signatus is a vector for the transmission of bacterial pathogenic bacteria Serratia marcescens (Bizio; Enterobacteriales: Enterobacteriaceae) that rots cotton seeds and bolls ( Glover et al, 2020 ). Further research is needed to determine whether A.suturalis is a vector for the bacteria.…”

Section: Discussionmentioning

confidence: 99%

Gut Bacterial Diversity in Different Life Cycle Stages of Adelphocoris suturalis (Hemiptera: Miridae)

et al. 2021

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Bacteria and insects have a mutually beneficial symbiotic relationship. Bacteria participate in several physiological processes such as reproduction, metabolism, and detoxification of the host. Adelphocoris suturalis is considered a pest by the agricultural industry and is now a major pest in cotton, posing a serious threat to agricultural production. As with many insects, various microbes live inside A. suturalis. However, the microbial composition and diversity of its life cycle have not been well-studied. To identify the species and community structure of symbiotic bacteria in A. suturalis, we used the HiSeq platform to perform high-throughput sequencing of the V3–V4 region in the 16S rRNA of symbiotic bacteria found in A. suturalis throughout its life stages. Our results demonstrated that younger nymphs (1st and 2nd instar nymphs) have higher species richness. Proteobacteria (87.06%) and Firmicutes (9.43%) were the dominant phyla of A. suturalis. At the genus level, Erwinia (28.98%), Staphylococcus (5.69%), and Acinetobacter (4.54%) were the dominant bacteria. We found that the relative abundance of Erwinia was very stable during the whole developmental stage. On the contrary, the relative abundance of Staphylococcus, Acinetobacter, Pseudomonas, and Corynebacterium showed significant dynamic changes at different developmental stages. Functional prediction of symbiotic bacteria mainly focuses on metabolic pathways. Our findings document symbiotic bacteria across the life cycle of A. suturalis, as well as differences in both the composition and richness in nymph and adult symbiotic bacteria. Our analysis of the bacteria in A. suturalis provides important information for the development of novel biological control strategies.

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Section: Discussionmentioning

confidence: 99%

Gut Bacterial Diversity in Different Life Cycle Stages of Adelphocoris suturalis (Hemiptera: Miridae)

et al. 2021

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“…Upon first bloom, groupings of four plants were caged using large organza fabric (152 × 122 cm, 240 JoAnn’s Fabrics, Hudson, OH, USA) according to their assigned treatment. These whole plant cages were successful in not inhibiting plant growth while excluding insects in other studies involving plant bugs [ 21 ]. Plants designated as experimental controls were left uncaged throughout the experiment and were exposed to any local pollinators.…”

Section: Methodsmentioning

confidence: 99%

A Native Bee, Melissodes tepaneca (Hymenoptera: Apidae), Benefits Cotton Production

Esquivel

Coulson

Brewer

2020

Insects

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The cotton agroecosystem is one of the most intensely managed, economically and culturally important cropping systems worldwide. Native pollinators are essential in providing pollination services to a diverse array of crops, including those which have the ability to self-pollinate. Cotton, which is autogamous, can potentially benefit from insect-mediated pollination services provided by native bees within the agroecosystem. Examined through two replicated experiments over two years, we hypothesized that native bees facilitated cross-pollination, which resulted in increased lint of harvested bolls produced by flowers exposed to bees and overall lint weight yield of the plant. Cotton bolls from flowers that were caged and exposed to bees, flowers that were hand-crossed, and bolls from flowers on uncaged plants exposed to pollinators had higher pre-gin weights and post-gin weights than bolls from flowers of caged plants excluded from pollinators. When cotton plants were caged with the local native bee Melissodes tepaneca, seed cotton weight was 0.8 g higher on average in 2018 and 1.18 g higher on average in 2019 than when cotton plants were excluded from bees. Cotton production gains from flowers exposed to M. tepaneca were similar when measuring lint and seed separately. Cotton flowers exposed over two weeks around the middle of the blooming period resulted in an overall yield gain of 12% to 15% on a whole plant basis and up to 24% from bolls produced from flowers exposed directly to M. tepaneca. This information complements cotton-mediated conservation benefits provided to native pollinators by substantiating native bee-mediated pollination services provided to the cotton agroecosystem.

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“…In insects, it has been identified in members of Coleoptera in various geographic regions in the world [ 26 , 27 , 28 , 29 , 30 ], including in the adult boll weevil Athonomis grandis [ 31 ]. Other host examples of S. marcescens are the verde plant bug Creontiades signatus (Distant) in S-Texas [ 32 ], Protaetia brevitarsis seulensis (Kolbe) in Korea [ 33 ], together withvarious other insects, like silkworms [ 34 ] and lepidoptera [ 35 , 36 , 37 ]. The strain Ss1 of S. marcescens has been described in mites, Metaseiulus occidentalis (Acari: Phytoseiidae) [ 38 ], and as a new pathogen for honey bees, Apis mellifera [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Serratia marcescens Strain Isolated from Yellow Mealworms, Tenebrio molitor, in The Netherlands

Bello Gonzalez,

van Gelderen,

Harders

et al. 2023

Insects

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Insect culture has developed rapidly worldwide; it faces important security and safety control issues, including animal infections and disease development. In the Netherlands, in 2021, a ~30% mortality of mealworms, Tenebrio molitor, occurred at one farm, where over-humid sites in the substrate were observed. Bacterial cultures from both the external and internal partsof fry and larger mealworms were identified by MALDI-TOF to predominantly Serratia marcescens, Staphylococcus xylosus and Staphylococus saprofyticus. Due to the important role of S. marcescens as a potential zoonotic bacterium, we performed a molecular characterization of the isolated strain. Genomic analysis showed a multidrug-resistant S. marcescens isolate carrying a tet (41), aac (6′)-Ic, and blaSST-1 chromosomal class C beta-lactamase-resistantgenes, all located on the chromosome. Additionally, several virulence genes were identified. The phylogenetic tree revealed that the S. marcescens strain from this study was similar to other S. marcescens strains from different ecological niches. Although the entomopathogenic activity was not confirmed, this case demonstrates that T. molitor can act as a reservoir and as an alternative path for exposing clinically important antibiotic-resistant bacteria that can affect animals and humans. It underlines the need to keep management factors optimal, before insects and their products enter the feed and food chain.

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Transmission of Cotton Seed and Boll Rotting Bacteria by the Verde Plant Bug (Hemiptera: Miridae)

Cited by 7 publications

References 14 publications

Gut Bacterial Diversity in Different Life Cycle Stages of Adelphocoris suturalis (Hemiptera: Miridae)

Gut Bacterial Diversity in Different Life Cycle Stages of Adelphocoris suturalis (Hemiptera: Miridae)

A Native Bee, Melissodes tepaneca (Hymenoptera: Apidae), Benefits Cotton Production

Molecular Characterization of Serratia marcescens Strain Isolated from Yellow Mealworms, Tenebrio molitor, in The Netherlands

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