Transmission ofHelicobacter pyloribetween a human and two dogs: A case report

Kubota-Aizawa, Sanae; Matsubara, Yasuo; Kanemoto, Hideyuki; Mimuro, Hitomi; Uchida, Kazuyuki; Chambers, James; Tsuboi, Masamichi; Ohno, Koichi; Fukushima, Kenjiro; Kato, Naoya; Yotsuyanagi, Hiroshi; Tsujimoto, Hajime

doi:10.1111/hel.12798

Cited by 20 publications

(23 citation statements)

References 30 publications

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“…DNA of zoonotic Helicobacter spp. were detected in the pigs’ and wild boars’ stomach samples screened in this study, so the close contact between wildlife, domestic animals, and humans should be a concern for the transmission of bacteria with zoonotic potential that raises awareness in a One health perspective [ 4 , 5 , 19 , 21 , 24 – 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Two PCR assays were used for detection of H. pylori DNA, one targeting the ureAB gene and the other one targeting the glmM gene. Although a number of studies have been published diagnosing H. pylori infection based on a ureAB gene PCR only [ 19 , 37 , 38 ], some reports indeed claim that H. pylori identification should include these two different target genes amplification [ 39 – 41 ]. Lu et al compared five PCR methods for detection of H. pylori DNA of 24 culture-positive samples obtained from 50 human gastric samples, including PCRs targeting the ureA and glmM gene.…”

Section: Discussionmentioning

confidence: 99%

“…Several Helicobacter spp. have been described to have a zoonotic potential and therefore close contact between humans, domestic animals, and wild animals deserves more attention [ 19 – 23 ]. Although reservoirs of wild and domestic animals can be considered important sources of emerging infectious diseases, it is the human impact on ecological systems that determines the level of risk at the human/animal interface upon the occurrence of emerging zoonotic diseases [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection of Helicobacter spp. and Fusobacterium gastrosuis in pigs and wild boars and its association with gastric histopathological alterations

Nunes

Mateus

Taillieu

et al. 2022

Vet Res

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Besides Helicobacter pylori, a Gram-negative bacterium that may cause gastric disorders in humans, non-Helicobacter pylori helicobacters (NHPH) may also colonize the stomach of humans and animals. In pigs, H. suis can induce gastritis and may play a role in gastric ulcer disease, possibly in association with Fusobacterium gastrosuis. In the present study, gastric samples from 71 slaughtered pigs and 14 hunted free range wild boars were tested for the presence of DNA of F. gastrosuis and gastric Helicobacter species associated with pigs, dogs cats and humans, using species-specific PCR assays, followed by sequencing of the amplicon. These gastric samples were also histopathologically evaluated. Almost all the pigs presented gastritis (95.8%). Helicobacter spp. were detected in 78.9% and F. gastrosuis in 35.2% of the animals. H. suis was the most frequently identified Helicobacter species (57.7% of the animals), followed by a H. pylori-like species (50.7%) and less often H. salomonis and H. felis (each in 2.8% of the animals). H. suis was most often detected in the glandular (distal) part of the stomach (pars oesophagea 9.9%, oxyntic mucosa 35.2%, antral mucosa 40.8%), while the H. pylori-like species was mainly found in the non-glandular (proximal) part of the stomach (pars oesophagea 39.4%, oxyntic mucosa 14.1%, antral mucosa 4.2%). The great majority of wild boars were also affected with gastritis (71.4%) and Helicobacter spp. and F. gastrosuis were detected in 64.3% and 42.9% of the animals, respectively. H. bizzozeronii and H. salomonis were the most frequently detected Helicobacter species, while a H. pylori-like species and H. suis were only occasionally identified. These findings suggest that these microorganisms can colonize the stomach of both porcine species and may be associated with gastric pathology. This should, however, be confirmed through bacterial isolation. This is the first description of the presence of F. gastrosuis DNA in the stomach of wild boars and a H. pylori-like species in the pars oesophagea of the porcine stomach.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular detection of Helicobacter spp. and Fusobacterium gastrosuis in pigs and wild boars and its association with gastric histopathological alterations

Nunes

Mateus

Taillieu

et al. 2022

Vet Res

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“…We also found that the rate of colonization in the Mongolian gerbils infected with H. pylori was 100% after 10 weeks. Furthermore, Mongolian gerbils have been kept as pets for children, and the transmission of H. pylori between humans and pets has been reported [ 24 ]. It is necessary to determine the prevalence of H. pylori in pet gerbils through noninvasive detection methods.…”

Section: Discussionmentioning

confidence: 99%

Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China

Zhang

Wang

et al. 2022

BioMed Research International

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Identifying Helicobacter pylori (H. pylori, Hp) infection in animals before and after artificial infection influences the subsequent experiment. We established effective and noninvasive detection methods, including the gastric fluid nested polymerase chain reaction (PCR) method and the 13C-urea breath test, which can detect Hp before modeling Hp infection in Mongolian gerbils. We designed a gas collection equipment for gerbils. Hp nested PCR was also performed on gastric fluid, gastric mucosa, duodenal contents, and faeces of gerbils challenged with Hp. Conventional Hp detection methods, including rapid urease assay and immunohistochemistry, were compared. Moreover, we assessed the natural infection of Hp in 135 gerbils that had never been exposed to Hp artificially from the major laboratory gerbil groups in China. In 10 Hp infected gerbils, the positive detection results were 100%, 100%, 90%, and 10% in gastric fluid, gastric mucosa, duodenal contents, and faeces with nested PCR, respectively. A rapid urease test performed on gastric mucosa showed that all animals were infected with Hp. Immunohistochemical detection and bacteria culture of gastric mucosa samples that were positive by the nested PCR method also confirmed the presence of Hp. 9% (3/35) and 6% (2/31) natural infection rates were found in conventional gerbil groups from the Capital Medical University and Zhejiang Laboratory Animal Center. In conclusion, we established two noninvasive Hp detection methods that can be performed before modelingHp infection, including the gastric fluid nested PCR method and the 13C-urea breath test.

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“…, Elhelw et al [32] performed PCR tests and reported that 62.5% of Egyptian dog stool samples were positive for H. pylori. Kubota-Aizawa et al [33] reported that gastric biopsies collected from two dogs in Japan were infected with the same H. pylori strain detected in gastric biopsies from their owners.…”

Section: Discussionmentioning

confidence: 99%

Identification and Prevalence of Helicobacter Pylori Virulence Genes Baba and Caga in Wolinella Isolates from the Oral Cavity of Dogs

Jahanshiri¹,

Nayeri-fasaei²,

Jamshidi³

et al. 2022

Preprint

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Wolinella spp. and Helicobacter spp. have been repeatedly reported in the oral cavity of dogs and are associated with periodontal disease. Wolinella strains predominate in the oral cavity of dogs. The only known species of this genus, Wolinella succinogenes, was considered non-pathogenic until sequence analysis of its genome revealed homologous genes resembling virulence factors in Helicobacter pylori. This has led researchers to question the nonpathogenic status of W. succinogenes. The cagA and babA genes are examples of crucial virulence factors in H. pylori pathogenesis; thus, the present study evaluated the prevalence of these genera and assessed the Wolinella strain genome in terms of the presence of these virulence factors. Multiple specific PCR tests were performed on oral secretion samples collected from 62 dogs by sterile cytobrush to evaluate the genera, species, and presence of virulence genes. The species-specific 16s rRNA genes from the Helicobacter and Wolinella genera were detected in 58.06% and 83.87% of the oral samples, respectively. H. pylori were not detected in the specimens. No cagA and babA genes were detected in the Wolinella spp. or non-pylori Helicobacter genomes. Our results confirmed that Wolinella spp. is the predominant population compared to Helicobacter in the oral cavity of dogs. Apparently, the incidence of Helicobacter infections is generally associated with non-pylori Helicobacter organisms. Despite the hypothesis of genomic homology between W. succinogenes and H. pylori, cagA and babA virulence genes were not identified in any of the oral samples from the dogs.

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Transmission ofHelicobacter pyloribetween a human and two dogs: A case report

Cited by 20 publications

References 30 publications

Molecular detection of Helicobacter spp. and Fusobacterium gastrosuis in pigs and wild boars and its association with gastric histopathological alterations

Molecular detection of Helicobacter spp. and Fusobacterium gastrosuis in pigs and wild boars and its association with gastric histopathological alterations

Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China

Identification and Prevalence of <i>Helicobacter Pylori</i> Virulence Genes <i>Baba</i> and <i>Caga</i> in <i>Wolinella</i> Isolates from the Oral Cavity of Dogs

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