Transparent, tough collagen laminates prepared by oriented flow casting, multi-cyclic vitrification and chemical cross-linking

Tanaka, Yuji; Baba, Koichi; Duncan, Thomas; Kubota, Akira; Asahi, Toru; Quantock, Andrew J.; Yamato, Masayuki; Okano, Teruo; Nishida, Kohji

doi:10.1016/j.biomaterials.2010.11.011

Cited by 35 publications

(30 citation statements)

References 51 publications

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“…As the main load bearing component in connective tissues, collagen has been extensively studied in cornea tissue engineering [13][14][15][16][17]. Most of the present researches in this area focus on the gelatin because of its biocompatibility and low antigenicity [18].…”

Section: Introductionmentioning

confidence: 99%

Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Liu

Wang

2013

Materials Science and Engineering: C

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Section: Introductionmentioning

confidence: 99%

Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Liu

Wang

2013

Materials Science and Engineering: C

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“…The step-by-step assembly of polyelectrolyte films [5][6][7][8] is another strategy to build innovative COL-based architectures that mimic extracellular matrix structures with fibrillar texture, which is of main importance for tissue homeostasis. [9][10][11][12][13][14] To build highly aligned structures made of synthetic polymer fibers or proteins, several strategies, motivated by the idea that an organized scaffold provides topographic cues to adherent cells, 15 have been developed: magnetic field, [16][17][18][19] electrospinning, 15,[20][21][22][23][24] micropatterning of surfaces, 25,26 buckling patterning of polydimethylsiloxane (PDMS) and UV/Ozone treatment, 27 use of microfluidic devices, 28 flow processing, 29 or even combined techniques such as electrospinning associated with a magnetic field. 17 These techniques were mainly used to induce alignment of cells, 15,[30][31][32] which in turn may cause changes in gene expression.…”

Section: Introductionmentioning

confidence: 99%

Cell Alignment Driven by Mechanically Induced Collagen Fiber Alignment in Collagen/Alginate Coatings

Chaubaroux

Perrin-Schmitt

Senger

et al. 2015

Tissue Engineering Part C: Methods

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For many years it has been a major challenge to regenerate damaged tissues using synthetic or natural materials. To favor the healing processes after tendon, cornea, muscle, or brain injuries, aligned collagen-based architectures are of utmost interest. In this study, we define a novel aligned coating based on a collagen/alginate (COL/ALG) multilayer film. The coating exhibiting a nanofibrillar structure is cross-linked with genipin for stability in physiological conditions. By stretching COL/ALG-coated polydimethylsiloxane substrates, we developed a versatile method to align the collagen fibrils of the polymeric coating. Assays on cell morphology and alignment were performed to investigate the properties of these films. Microscopic assessments revealed that cells align with the stretched collagen fibrils of the coating. The degree of alignment is tuned by the stretching rate (i.e., the strain) of the COL/ALG-coated elastic substrate. Such coatings are of great interest for strategies that require aligned nanofibrillar biological material as a substrate for tissue engineering.

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“…In order to achieve the purpose of the bionic, most of the cornea substrate material is still using collagen. Recently the researches in the field of collagen‐based corneal substitutes are focused on orientating the collagen fibers and fabricating the composite materials as corneal substitutes like with polyethylene glycol (PEG), polyvinyl alcohol (PVA), etc., to obtain good optical and mechanical characters.…”

Section: Introductionmentioning

confidence: 99%

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

Long

Liu

et al. 2014

J Biomedical Materials Res

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Although collagen with outstanding biocompatibility has promising application in corneal tissue engineering, the mechanical properties of collagen-based scaffolds, especially suture retention strength, must be further improved to satisfy the requirements of clinical applications. This article describes a toughness reinforced collagen-based membrane using silk fibroin. The collagen-silk fibroin membranes based on collagen [silk fibroin (w/w) ratios of 100:5, 100:10, and 100:20] were prepared by using silk fibroin and cross-linking by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. These membranes were analyzed by scanning electron microscopy and their optical property, and NaCl and tryptophan diffusivity had been tested. The water content was found to be dependent on the content of silk fibroin, and CS10 membrane (loading 10 wt % of silk fibroin) performed the optimal mechanical properties. Also the suture experiments have proved CS10 has high suture retention strength, which can be sutured in rabbit eyes integrally. Moreover, the composite membrane proved good biocompatibility for the proliferation of human corneal epithelial cells in vitro. Lamellar keratoplasty shows that CS10 membrane promoted complete epithelialization in 35 ± 5 days, and their transparency is restored quickly in the first month. Corneal rejection reaction, neovascularization, and keratoconus are not observed. The composite films show potential for use in the field of corneal tissue engineering.

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Transparent, tough collagen laminates prepared by oriented flow casting, multi-cyclic vitrification and chemical cross-linking

Cited by 35 publications

References 51 publications

Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Cell Alignment Driven by Mechanically Induced Collagen Fiber Alignment in Collagen/Alginate Coatings

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

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