Transplantation of an alginate–matrigel matrix containing isolated ovarian cells: First step in developing a biodegradable scaffold to transplant isolated preantral follicles and ovarian cells

Vanacker, Julie; Luyckx, Valérie A.; Dolmans, Marie-Madeleine; Rieux, Anne des; Jaeger, Jonathan; Langendonckt, Anne Van; Donnez, Jacques; Amorim, Christiani Andrade

doi:10.1016/j.biomaterials.2012.05.015

Cited by 145 publications

(112 citation statements)

References 34 publications

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“…The need to find alternative fertility-restoring solutions for these patients led us to investigate isolated follicle grafting and launch the artificial ovary concept. Follicles isolated from frozen-thawed ovarian tissue are embedded in 3-D matrices, such as plasma clots [24,25], alginate [26] or fibrin [27,28], along with ovarian stromal and endothelial cells, which allow formation of a well vascularized ovary-like structure [29]. This artificial ovary provides a more natural environment for follicle survival and development.…”

Section: Discussionmentioning

confidence: 99%

“…The number of grafted leukemic cells grafted was based on results from a previous study using a model of artificially contaminated ovarian tissue, in which between 1 and 97 leukemic cells were found in the follicle suspension (Soares et al, submitted). We embedded these leukemic cells in a fibrin matrix along with 50,000 ovarian cells, as in our previous studies on the artificial ovary [26,28]. In a clinical setting, the artificial ovary would be grafted, like cryopreserved ovarian cortex, either to the remaining ovary and/or inside a peritoneal window, both sites having proved equally effective [41,42].…”

Section: Discussionmentioning

confidence: 99%

“…Fourteen 6-to 9-week-old female SCID mice (Charles River Laboratories) were used for this study. Housing conditions, anesthesia and analgesia were previously described [26]. After performing a ventral midline incision, a peritoneal pocket was created on the right inner side of the peritoneum using nonabsorbable 6-0 Prolene suture (Fig.…”

Section: Xenografting Of Fibrin Clots To Scid Micementioning

confidence: 99%

“…For this reason, our team has been working on the grafting of individual ovarian follicles, enzymatically isolated from frozen-thawed ovarian tissue and embedded in 3-D matrices [24][25][26][27][28]. Addition of ovarian stromal and endothelial cells to the matrix was found to be crucial for the formation of a well vascularized ovary-like structure [29].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Is transplantation of a few leukemic cells inside an artificial ovary able to induce leukemia in an experimental model?

Soares

Saussoy

Sahrari

et al. 2015

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Xenografting Of Fibrin Clots To Scid Micementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Is transplantation of a few leukemic cells inside an artificial ovary able to induce leukemia in an experimental model?

Soares

Saussoy

Sahrari

et al. 2015

J Assist Reprod Genet

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“…It is biocompatible and shows non antigenicity [4]. Alginate was also proposed as a candidate material for transplantable artificial ovary matrices in 2012 [3], [5]. It has also been reported that different combinations with alginate such as matrigel could be successfully employed for transplanting murine ovarian cells [3].…”

Section: Introductionmentioning

confidence: 99%

A Mechanical Model for an Artificial Oocyte

Hajiyavand¹,

Saadat²,

Stamboulis³

2017

IJMO

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Abstract-Cell injection to introduce a foreign material in biological cells is currently an important area of research. Intervention with biological cells such as an oocyte poses a major limitation towards understanding their biomechanical behaviour, especially during injection, which could result in significant and undesirable deformation of the outer membrane. In this paper, a cross-linked alginate hydrogel is proposed as a material to develop an artificial oocyte to simulate the mechanical properties of a natural human oocyte and use it for mechanical analysis and modelling. A mechanical model and finite element analysis are first presented. The relationship between the injection force and the mechanical properties of the gel are then studied and presented. To validate the mechanical model, experiments were performed using different diameters of an oocyte made using the proposed hydrogel, showing that the modelling results satisfy the experimental demands and that this model can be used to estimate the mechanical properties of other gels too.Index Terms-Artificial oocyte, alginate, hydrogel, finite element model, deformation. I. INTRODUCTIONMany researchers have focused on micro-injection technology which has been proved to be an effective technique introducing foreign materials into cells. This technology can be applied for gene injection, In-vitro fertilisation (IVF), intra-cytoplasmic sperm injection (ICSI), drug development and other biomedical areas notably in cancer treatments [1]. To the best knowledge of the authors, this is the first report of proposing a material for fabricating an artificial oocyte.Accessing human biological oocyte samples for experimental work is very limited, and therefore having suitable artificial samples that could mimic the natural tissues would help to develop appropriate experimental methodology.Synthetic and natural polymers could be considered for the development of artificial tissues. In 2017 alginate hydrogels were proposed for transplantation of ovarian follicles considering different grafting times [2]. Alginate is one of the proposed materials which has the minimum grafting time that is reported to be one week [3]. Alginate

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