Transport of membrane-bound macromolecules by M cells in follicle-associated epithelium of rabbit Peyer's patch

Abstract

M cells in Peyer's patch epithelium conduct transepithelial transport of luminal antigens to cells of the mucosal immune system. To determine the distribution of specific lectin-binding sites on luminal membranes of living M cells and to follow the transport route of membrane-bound molecules, lectin-ferritin conjugates and cationized ferritin were applied to rabbit Peyer's patch mucosa in vivo and in vitro. The degree to which binding enhances transport was estimated by comparing quantitatively the transport of an adherent probe, wheat germ agglutinin-ferritin, to that of a nonadherent BSA-colloidal gold probe. When applied to fixed tissue, the lectins tested bound equally well to M cells and columnar absorptive cells. On living mucosa, however, ferritin conjugates of wheat germ agglutinin and Ricinus communis agglutinins I and II bound more avidly to M cells. Absorptive cells conducted little uptake and no detectable transepithelial transport. Lectins on M cell membranes were endocytosed from coated pits, rapidly transported in a complex system of tubulocisternae and vesicles, and remained adherent to M cell basolateral membranes. Cationized ferritin adhered to anionic sites and was similarly transported, but was released as free clusters at M cell basolateral surfaces. When applied simultaneously to Peyer's patch mucosa, wheat germ agglutinin-ferritin was transported about 50 times more efficiently than was bovine serum albumin-colloidal gold.

Naturally Developing Memory T Cell Xenoreactivity to Swine Antigens in Human Peripheral Blood Lymphocytes

“…Although the reason for this finding remains unclear, it is well established that M cells in the gut can transport intact macromolecules across epithelial barriers (27,28). Thus, it is theoretically possible under such a scenario that oral intake of pork could lead to an interaction of M cell-associated, gut-infiltrating lymphocytes with directly presented SLA molecules to prime an immune response.…”

The sheep and cattle Peyer’s patch as a site of B-cell development

“…Large particles and bacteria induce phagocytosis that is associated with rearrangement of the actin cytoskeleton and this permits active formation of pseudopod-like structures [15,33]. Viruses and adherent particles are taken up by endocytosis, but non-adherent materials are internalized by fluid phase endocytosis [14,74,81]. We are a long way from understanding the origin, differentiation, and fate of FAE in ileal PP.…”

Isolation and Biochemical Characterization of Mucus Proteins in Japanese Bunching Onion (<i>Allium fistulosum</i>) Green Leaves

“…Although further studies are required to understand the mechanism, M cells are plausible targets for the orally administered mucus containing the lectin and TLPs. M cells express particular glycosylation patterns on their surfaces and transport a wide range of molecules from the intestinal lumen to the underlying lymphoid tissue, where a local and systemic potent immune response is initiated (Neutra et al, 1987). It has been reported that some plant lectins are able to induce T helper cell type 1 (Th1) cytokines upon interaction with glycosylated receptors on macrophages and/or dendritic cells, such as type 2 and 4 Tolllike receptors (TLR2 and TLR4), respectively (Souza et al, 2013).…”