Transposase and cointegrase: specialized transposition proteins of the bacterial insertion sequence IS21 and related elements

Berger, Bernard; Haas, Dieter

doi:10.1007/pl00000866

Cited by 49 publications

(63 citation statements)

References 125 publications

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“…In this view, the IS element FK1 co-transferred with nod genes to the recipient (Fig. 1B) belongs to the IS21 family 3,13,14) . A minicircle containing IS21 copies has been detected in preparations of plasmid DNA carrying IS21, raising the possibility that IS21 is transposed together with other DNA fragments via a circular intermediate 3) .…”

Section: Discussionmentioning

confidence: 98%

“…1B) belongs to the IS21 family 3,13,14) . A minicircle containing IS21 copies has been detected in preparations of plasmid DNA carrying IS21, raising the possibility that IS21 is transposed together with other DNA fragments via a circular intermediate 3) . This together with the unstable behavior of the acquired nod genes during successive cultures, suggests the IS element FK1 forms a minicircle intermediate carrying nod genes.…”

Section: Discussionmentioning

confidence: 99%

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Horizontal Transfer of Nodulation Genes in Soils and Microcosms from Bradyrhizobium japonicum to B. elkanii.

et al. 2002

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We investigated the horizontal transfer of nodulation (nod) genes to a Bradyrhizobium elkanii strain, lacking common nod genes as a recipient, in soils and microcosms using selection systems of antibiotic resistance and legume nodulation. We observed the horizontal transfer of nod genes at 4°C in Nakazawa soil where peculiar strains (HRS strains) of B. japonicum harboring high copy numbers of insertion sequences dominated. In microcosms containing HRS strains as donors, we detected a similar horizontal transfer from B. japonicum HRS strain NK5 to the B. elkanii recipient more efficiently at 4°C, which was verified by examining hybridization, nodulation and Nod factor production. These traits were, however, gradually lost during successive cultures. Plasmid analysis indicated that this event was not due to the simple transfer of plasmid carrying common nod genes. These results suggest the potential for horizontal transfer of nod genes among bradyrhizobia and other bacterial populations in soil environments.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Horizontal Transfer of Nodulation Genes in Soils and Microcosms from Bradyrhizobium japonicum to B. elkanii.

et al. 2002

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“…Both IstA and IstB are required for efficient transposition whereas a product of an alternative translation initiation within the Tpase gene may facilitate integration. Using IstA-enriched Escherichia coli cell extracts, it was shown that this protein is responsible for 3′ end cleavage of IS21 and of both ends in the IR junction (177).…”

Section: Is21 (I) Generalmentioning

confidence: 99%

Everyman's Guide to Bacterial Insertion Sequences

et al. 2015

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The number and diversity of known prokaryotic insertion sequences (IS) have increased enormously since their discovery in the late 1960s. At present the sequences of more than 4000 different IS have been deposited in the specialized ISfinder database. Over time it has become increasingly apparent that they are important actors in the evolution of their host genomes and are involved in sequestering, transmitting, mutating and activating genes, and in the rearrangement of both plasmids and chromosomes. This review presents an overview of our current understanding of these transposable elements (TE), their organization and their transposition mechanism as well as their distribution and genomic impact. In spite of their diversity, they share only a very limited number of transposition mechanisms which we outline here. Prokaryotic IS are but one example of a variety of diverse TE which are being revealed due to the advent of extensive genome sequencing projects. A major conclusion from sequence comparisons of various TE is that frontiers between the different types are becoming less clear. We detail these receding frontiers between different IS-related TE. Several, more specialized chapters in this volume include additional detailed information concerning a number of these.In a second section of the review, we provide a detailed description of the expanding variety of IS, which we have divided into families for convenience. Our perception of these families continues to evolve and families emerge regularly as more IS are identified. This section is designed as an aid and a source of information for consultation by interested specialist readers.

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“…It has been suggested that covalently closed IS circles and dimers could be alternative representations of the same transpositional pathway (Lewis & Grindley, 1997;Kiss & Olasz, 1999). Recent studies (Berger & Haas, 2001;Prudhomme et al, 2002;Turlan et al, 2000) seem to further support this possibility.…”

Section: Discussionmentioning

confidence: 94%

Detection and analysis of transpositionally active head-to-tail dimers in three additional Escherichia coli IS elements

et al. 2003

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This study demonstrates that Escherichia coli insertion elements IS3, IS150 and IS186 are able to form transpositionally active head-to-tail dimers which show similar structure and transpositional activity to the dimers of IS2, IS21 and IS30. These structures arise by joining of the left and right inverted repeats (IRs) of two elements. The resulting junction includes a spacer region (SR) of a few base pairs derived from the flanking sequence of one of the reacting IRs. Head-to-tail dimers of IS3, IS150 and IS186 are unstable due to their transpositional activity. They can be resolved in two ways that seem to form a general rule for those elements reported to form dimers. One way is a sitespecific process (dimer dissolution) which is accompanied by the loss of one IS copy along with the SR. The other is 'classical' transposition where the joined ends integrate into the target DNA. In intramolecular transposition this often gives rise to deletion formation, whereas in intermolecular transposition it gives rise to replicon fusion. The results presented for IS3, IS150 and IS186 are in accordance with the IS dimer model, which is in turn consistent with models based on covalently closed minicircles.

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Transposase and cointegrase: specialized transposition proteins of the bacterial insertion sequence IS21 and related elements

Cited by 49 publications

References 125 publications

Horizontal Transfer of Nodulation Genes in Soils and Microcosms from Bradyrhizobium japonicum to B. elkanii.

Horizontal Transfer of Nodulation Genes in Soils and Microcosms from Bradyrhizobium japonicum to B. elkanii.

Everyman's Guide to Bacterial Insertion Sequences

Detection and analysis of transpositionally active head-to-tail dimers in three additional Escherichia coli IS elements

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