Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis

Buehler, Adrian; Brown, Emma; Paulus, Lars-Philip; Eckstein, Markus; Thoma, Oana‐Maria; Oraiopoulou, Mariam-Eleni; Rother, Ulrich; Hoerning, André; Hartmann, Arndt; Woelfle, Joachim; Friedrich, Oliver; Waldner, Maximilian J.; Knieling, Ferdinand; Bohndiek, Sarah E.; Regensburger, Adrian P.

doi:10.1002/advs.202300564

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…In murine models of colitis, disease severity can be measured by means of increased signal intensities for hemoglobin transabdominally in vivo [118,119], and by using transrectal guidance, changes in the intestinal wall thickness and vessel architecture can even be detected [120]. Furthermore, OAI technologies have the potential to identify intestinal inflammation and fibrosis to characterize intestinal strictures in mice [121,122].…”

Section: Novel Us-based Imaging Technologies: Optoacoustic Imaging (Oai)mentioning

confidence: 99%

Ultrasound in Pediatric Inflammatory Bowel Disease—A Review of the State of the Art and Future Perspectives

Hoerning,

Jüngert,

Siebenlist

et al. 2024

Children

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Inflammatory bowel disease (IBD) comprises a group of relapsing, chronic diseases of the gastrointestinal tract that, in addition to adults, can affect children and adolescents. To detect relapses of inflammation, these patients require close observation, frequent follow-up, and therapeutic adjustments. While reference standard diagnostics include anamnestic factors, laboratory and stool sample assessment, performing specific imaging in children and adolescents is much more challenging than in adults. Endoscopic and classic cross-sectional imaging modalities may be invasive and often require sedation for younger patients. For this reason, intestinal ultrasound (IUS) is becoming increasingly important for the non-invasive assessment of the intestine and its inflammatory affection. In this review, we would like to shed light on the current state of the art and provide an outlook on developments in this field that could potentially spare these patients more invasive follow-up procedures.

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Section: Novel Us-based Imaging Technologies: Optoacoustic Imaging (Oai)mentioning

confidence: 99%

Ultrasound in Pediatric Inflammatory Bowel Disease—A Review of the State of the Art and Future Perspectives

Hoerning,

Jüngert,

Siebenlist

et al. 2024

Children

Self Cite

View full text Add to dashboard Cite

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Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis

et al. 2023

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Optoacoustic imaging (OAI) enables microscale imaging of endogenous chromophores such as hemoglobin at significantly higher penetration depths compared to other optical imaging technologies. Raster‐scanning optoacoustic mesoscopy (RSOM) has recently been shown to identify superficial microvascular changes associated with human skin pathologies. In animal models, the imaging depth afforded by RSOM can enable entirely new capabilities for noninvasive imaging of vascular structures in the gastrointestinal tract, but exact localization of intra‐abdominal organs is still elusive. Herein the development and application of a novel transrectal absorber guide for RSOM (TAG‐RSOM) is presented to enable accurate transabdominal localization and assessment of colonic vascular networks in vivo. The potential of TAG‐RSOM is demonstrated through application during mild and severe acute colitis in mice. TAG‐RSOM enables visualization of transmural vascular networks, with changes in colon wall thickness, blood volume, and OAI signal intensities corresponding to colitis‐associated inflammatory changes. These findings suggest TAG‐RSOM can provide a novel monitoring tool in preclinical IBD models, refining animal procedures and underlines the capabilities of such technologies to address inflammatory bowel diseases in humans.

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In Vivo Assessment of Deep Vascular Patterns in Murine Colitis Using Optoacoustic Mesoscopic Imaging

Buehler,

Brown,

Nedoschill

et al. 2024

Advanced Science

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The analysis of vascular morphology and functionality enables the assessment of disease activity and therapeutic effects in various pathologies. Raster‐scanning optoacoustic mesoscopy (RSOM) is an imaging modality that enables the visualization of superficial vascular networks in vivo. In murine models of colitis, deep vascular networks in the colon wall can be visualized by transrectal absorber guide raster‐scanning optoacoustic mesoscopy (TAG‐RSOM). In order to accelerate the implementation of this technology in translational studies of inflammatory bowel disease, an image‐processing pipeline for TAG‐RSOM data has been developed. Using optoacoustic data from a murine model of chemically‐induced colitis, different image segmentation methods are compared for visualization and quantification of deep vascular patterns in terms of vascular network length and complexity, blood volume, and vessel diameter. The presented image‐processing pipeline for TAG‐RSOM enables label‐free in vivo assessment of changes in the vascular network in murine colitis with broad applications for inflammatory bowel disease research.

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Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis

Cited by 3 publications

References 53 publications

Ultrasound in Pediatric Inflammatory Bowel Disease—A Review of the State of the Art and Future Perspectives

Ultrasound in Pediatric Inflammatory Bowel Disease—A Review of the State of the Art and Future Perspectives

Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis

In Vivo Assessment of Deep Vascular Patterns in Murine Colitis Using Optoacoustic Mesoscopic Imaging

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