Transumbilical endoscopic cholecystectomy in a porcine model

Zhu, Hongyi; Fu, Li; Li, Kewei; Zhang, Xiwen; Wang, Jian; Ji, Fu

doi:10.1590/s0102-86502013001100003

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…The foundations of the current study were: the important role of serotonin synthesized by intestinal ECs and mast cells established mainly in laboratory animals and man; the scarce information on the distribution of ECs and serotonin-producing mast cells in the wall of the extrahepatic bile ducts (EHBD), major duodenal papilla (PDM), and gallbladder (GB) in the domestic pig as preferred experimental species for various morphological, physiological and biochemical studies (41,42); and the bile ducts related surgeries (43).…”

Section: Introductionmentioning

confidence: 99%

Serotonin Immunoreactive Cells in Extrahepatic Bile Ducts, Major Duodenal Papilla and Gallbladder in the Domestic Pig

Stefanov

2024

Macedonian Veterinary Review

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The main part of serotonin in the body is synthesized and released by a certain type of enteroendocrine cells in the intestinal mucosa called enterochromaffin cells. The scarce qualitative and quantitative data on enterochromaffin and serotonin-positive mast cells in porcine extrahepatic bile ducts and gallbladder, motivated us to undertake the present study. The aim of this study was to determine the localization and density of serotonin-positive cells in the wall of the extrahepatic bile ducts and gallbladder in pigs. An immunohistochemical method was used to identify enterochromaffin cells and determine their percentage relative to the total number of endocrine cells labeled with chromogranin A. Serotonin-positive mast cells were identified after tryptase staining of serial sections. The endocrine function of mast cells was demonstrated by chromogranin A immunolabeling. The highest number of enterochromaffin cells were found in the intramural part of the ductus choledochus, followed by the papilla duodeni major, extramural part of the ductus choledochus, ductus hepaticus comunis, ductus cysticus, and gallbladder. In all parts of the extrahepatic bile ducts, the highest number of mast cells was found in the muscle layer, followed by the serosal layer and the propria. The expression of serotonin in the enterochromaffin cells of the biliary glands and in the mast cells of the analyzed organs suggests a possible synthesis of serotonin, which probably regulates physiological and pathological processes.

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Section: Introductionmentioning

confidence: 99%

Serotonin Immunoreactive Cells in Extrahepatic Bile Ducts, Major Duodenal Papilla and Gallbladder in the Domestic Pig

Stefanov

2024

Macedonian Veterinary Review

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Section: Introductionmentioning

confidence: 99%

“…The Calot`s triangle and hepatocystic triangle in humans are studied in detail because of the cholecystectomy surgery [18]. In order to develop new surgical techniques for gallbladder removing, the domestic swine is widely used as one of the most suitable experimental models for cholecystectomy [18]. Misinterpretation or lack of knowledge of this information contributes to intraoperative complications such as biliary injuries, which can cause serious morbidity and occasionally mortality [9].…”

Section: Introductionmentioning

confidence: 99%

Calot`s Triangle – and Hepatocystic triangle – Like Areas in Domestic Swine

Ivaylo¹,

Stefanov²

2022

AMA

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Due to the similarities to human anatomy the domestic swine is one of the most preferred species for experiments especially in developing new surgical techniques in the cholecystectomy in humans. The aim of the study was to establish the existence of both the Calot`s triangle and the hepatocystic triangle and the length of their borders in the domestic pigs. This research work was conducted on livers from 30 male pigs separated to different age groups. The results showed that Calot`s triangle -and the hepatocystic triangle-like areas were present. The borders of the triangles were identified, measured and statistically analyzed in immature and mature pigs and age dependent differences were identified. The similarities and differences in borders of triangles between pigs and humans were discussed. This study revealed that, like in humans, the Calot`s triangle and the hepatocystic triangle are presented and have important clinical significance.

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Endoscopic trans-esophageal submucosal tunneling surgery: A new therapeutic approach for diseases located around the aorta ventralis

Xiong

Chen

Chai

et al. 2019

WJG

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AIMTo assess the efficiency of endoscopic trans-esophageal submucosal tunneling surgery (EESTS) technique for diseases located around the aorta ventralis.METHODSNine pigs were assigned to EESTs. The procedures were as follows: First, a long esophageal submucosal tunnel was established. Second, full-thickness myotomy was created. Third, an endoscope was entered into the abdominal cavity through a muscle incision and the endoscope was around the aorta ventralis. Eventually, celiac trunk ganglion neurolysis, partial hepatectomy and splenectomy, partial tissue resection in the area of the posterior peritoneum, and endoscopic submucosal dissection (ESD) combined with lymph node dissection were performed. The animals were given antibiotics for 5 d and necropsied 7 d after surgery.RESULTSIn all surgeries, one pig died from intraperitoneal hemorrhage after doing partial splenectomy, while the other pigs were alive after successfully operating other surgeries. For surgery of celiac trunk ganglion damage, at necropsy, there was no exudation in the abdominal cavity. Regarding surgery of partial hepatectomy, the wound with part healing was observed in the left hepatic lobe, and no bleeding or obvious exudation was seen. In surgery of partial splenectomy, massive hemorrhage was observed on the splenic wound surface, and the metal clips could not stop bleeding. After surgery of retroperitoneal tissue resection, mild tissue adhesion was observed in the abdominal cavity of one animal, and another one suffered from severe infection. For surgery of ESD and lymph node dissection, a moderate tissue adhesion was observed.CONCLUSIONEESTS is a feasible and safe technique for diseases located around the aorta ventralis.

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Transumbilical endoscopic cholecystectomy in a porcine model

Cited by 3 publications

References 24 publications

Serotonin Immunoreactive Cells in Extrahepatic Bile Ducts, Major Duodenal Papilla and Gallbladder in the Domestic Pig

Serotonin Immunoreactive Cells in Extrahepatic Bile Ducts, Major Duodenal Papilla and Gallbladder in the Domestic Pig

Calot`s Triangle – and Hepatocystic triangle – Like Areas in Domestic Swine

Endoscopic trans-esophageal submucosal tunneling surgery: A new therapeutic approach for diseases located around the aorta ventralis

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