Transverse nuclear spin relaxation in phospholipid bilayer membranes

Bloom, Myer; Sternin, Edward

doi:10.1021/bi00382a007

Cited by 109 publications

(96 citation statements)

References 25 publications

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“…6B indicate that DNA induces a broadening of the band, with linewidths of respectively of 5.4 and 5.2 ppm in the presence of plasmidic and linear DNA (Table 1). This broadening effect could be due to a decrease of the T 2 values in the presence of DNA and could be explained by a faster lateral diffusion (Bloom and Sternin, 1987) of the lipids in the smaller lipid structures obtained in the presence of DNA.…”

Section: P Mas Solid-state Nmrmentioning

confidence: 98%

Spectroscopic characterization of DMPC/DOTAP cationic liposomes and their interactions with DNA and drugs

Labbé

Cronier

C.‐Gaudreault

et al. 2009

Chemistry and Physics of Lipids

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Section: P Mas Solid-state Nmrmentioning

confidence: 98%

Spectroscopic characterization of DMPC/DOTAP cationic liposomes and their interactions with DNA and drugs

Labbé

Cronier

C.‐Gaudreault

et al. 2009

Chemistry and Physics of Lipids

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“…Variation of the Larmor frequency (magnetic field) in the T 1 Z experiment, or pulse repetition rate in the T 2CPMG (CPMG, Carr-Purcell-Meiboom-Gill) [118,119] experiment, provides an experimental variable that is useful for testing motional models according to the frequency dependence of the spectral densities of motion [115,120,121]. Here, spin-lattice relaxation rates recorded as a function of Larmor frequency (field strength) enable one to test specific dynamic models [122,123].…”

Section: Nmr Relaxation Gives Dynamical Parameters That Characterimentioning

confidence: 99%

An NMR database for simulations of membrane dynamics

Leftin

Brown

2011

Biochimica et Biophysica Acta (BBA) - Biomembranes

102

119

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Computational methods are powerful in capturing the results of experimental studies in terms of force fields that both explain and predict biological structures. Validation of molecular simulations requires comparison with experimental data to test and confirm computational predictions. Here we report a comprehensive database of NMR results for membrane phospholipids with interpretations intended to be accessible by non-NMR specialists. Experimental 13C–1H and 2H NMR segmental order parameters (SCH or SCD) and spin-lattice (Zeeman) relaxation times (T1Z) are summarized in convenient tabular form for various saturated, unsaturated, and biological membrane phospholipids. Segmental order parameters give direct information about bilayer structural properties, including the area per lipid and volumetric hydrocarbon thickness. In addition, relaxation rates provide complementary information about molecular dynamics. Particular attention is paid to the magnetic field dependence (frequency dispersion) of the NMR relaxation rates in terms of various simplified power laws. Model-free reduction of the T1Z studies in terms of a power-law formalism shows that the relaxation rates for saturated phosphatidylcholines follow a single frequency-dispersive trend within the MHz regime. We show how analytical models can guide the continued development of atomistic and coarse-grained force fields. Our interpretation suggests that lipid diffusion and collective order fluctuations are implicitly governed by the viscoelastic nature of the liquid-crystalline ensemble. Collective bilayer excitations are emergent over mesoscopic length scales that fall between the molecular and bilayer dimensions, and are important for lipid organization and lipid-protein interactions. Future conceptual advances and theoretical reductions will foster understanding of biomembrane structural dynamics through a synergy of NMR measurements and molecular simulations.

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“…We recently introduced the QCPMG experiment, i.e., the ing pulses, the sensitivity of quadrupolar-echo MAS NMR spectra quadrupolar version (9, 10) of the Carr-Purcell-Meiboomfor the central transition of half-integer quadrupolar nuclei exhib-Gill (CPMG) (11, 12) experiment, as a means to increase iting large quadrupolar couplings may be significantly enhanced. the sensitivity of quadrupolar-echo (QE) spectra (13-15) this experiment has been used to distinguish inhomogeneous from homogeneous interactions in solid-state NMR (9, 10,(17)(18)(19)(20)(21)(22)(23)(24)(25), where the latter category include homonuclear diConsiderable interest in unraveling the combined effect polar coupling and molecular dynamics. For broad powder of different anisotropic nuclear spin interactions in solid-patterns induced by inhomogeneous second-order quadrupostate NMR spectroscopy has characterized the development lar coupling, it should be possible to further improve the of new experimental methods in recent years.…”

mentioning

confidence: 99%