Trapping mode two-dimensional liquid chromatography for quantitative low-level impurity enrichment in pharmaceutical development

Lin, Ziqing; Wang, Qinggang; Zhou, Yiyang; Shackman, Jonathan G.

doi:10.1016/j.chroma.2023.464043

Cited by 4 publications

(1 citation statement)

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“…Thus, appropriate analytical methods should be developed for controlling the quality of pharmaceutical analysis [ 8 , 12 ]. Various techniques like spectrophotometry, electroanalytical techniques (majorly voltammetry), titrimetric, fluorimetry, and chromatographic methods like TLC, GC, HPLC, and CE (capillary electrophoresis) are used for the identification and quantitative analysis of pharmaceutical drugs [ 1 , 5 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Novel HPLC Methods for Quantitative Determination of Vitamin D3 in Tablet Dosage Form

Gohar,

Rahman,

Bahadur

et al. 2024

Pharmaceuticals

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In the present work, an efficient isocratic HPLC method was developed for the precise and accurate estimation of vitamin D3 in tablet form. The chromatographic conditions comprised an L3 silica column (5 µm in particle size, 4.6 mm × 250 mm) with a mobile phase n-hexane/ethyl acetate (85:15 v/v) with a flow rate of 2.0 mL/min and a detection wavelength of 292 nm. The new methodology was validated for accuracy, precision, specificity, robustness, and quantification limits according to an official monograph of USP/BP and ICH guidelines. The peak areas of the six replicates of the homogeneous sample were recorded. The mean value obtained was 67,301, and the relative standard deviation (RSD) was 0.1741. The linearity and range were in the acceptable bounds, i.e., 0.999, which was calculated using regression line analysis. The results show that the method is truly acceptable as the RSD, as the flow rate was 0.81%, while for the mobile phase composition, it was 0.72%, which lies in the acceptable range. The limit of detection (LOD) and the limit of quantification (LOQ) values were 0.0539 µg/mL and 0.1633 µg/mL, respectively. The % RSD of the intra and inter-day precision of the method was deemed acceptable according to the international commission for harmonization guidelines. The developed method has potential to be used for the detection and quantification of vitamin D3 during routine analysis for tablets in dosage form.

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