Treating boar sperm with cholesterol-loaded cyclodextrins widens the sperm osmotic tolerance limits and enhances the in vitro sperm fertilising ability

Tomás, C.; Blanch, E.; Hernández, M.; Gil, M.A.; Roca, Jordi; Vázquez, Juan M.; Martı́nez, Emilio A.; Mocé, E.

doi:10.1016/j.anireprosci.2011.12.003

Cited by 45 publications

(43 citation statements)

References 52 publications

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“…The lowest progressive motility values were observed in 3% G (low-level G) groups both with CLC and 7-DHCLC addition (P<0.001). In addition, these results have shown that the 7-DHCLC addition has been more effective than CLC considering the cryotolerance of sperm cells, protection of their morphologic and metabolic properties from toxicity and high osmotic pressure that have been caused by G. Similar to our study, Thomas et al [22] have demonstrated that CLC treatment affects the sperm membrane permeability. In the current study, 7-DHCLC 9% G had higher mitochondrial activation compared to both 3G groups.…”

Section: Discussionsupporting

confidence: 79%

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Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

̇İnanç¹,

Olğaç²,

Tekin³

et al. 2018

Kafkas Univ Vet Fak Derg

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How to Cite This Articleİnanç ME, Olgac KT, Tekin K, Çil B, Alemdar H, Özen D, Uysal O: Effect of cholesterol and 7-dehydrocholesterol on bull semen freezing with different rates of glycerol. Kafkas Univ Vet Fak Derg, 24 (6): 815-820, 2018. DOI: 10.9775/kvfd.2018 AbstractThe aim of this study was to evaluate the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol loaded cyclodextrin (CLC) with the addition of different glycerol (G) rates on bull semen cryopreservation. Semen samples of three bulls were pooled and divided into seven groups; control group (C) and the other six were extended with standard Tris extender including different G rates (3, 6 and 9%) with CLC or 7-DHCLC to reach 1.5 mg/120x10 6 . Extended samples were frozen in 0.25 mL straws. After thawing, motion characteristics and motility, viability, acrosome integrity, mitochondrial activation, functional membrane integrity and abnormal spermatozoa rates were assessed. The highest membrane integrity, viability and total motility were detected in 7-DHCLC with 9% of G group (P<0.001). The lowest progressive motility was detected in 3% G groups (P<0.001). 7-DHCLC with 9% G had higher mitochondrial activity compared with 3% G groups. In addition, no statistical difference was observed between groups in terms of acrosome integrity and abnormal spermatozoa rates (P>0.05). According to these results, 7-DHCLC with 9% G addition maintained some spermatological parameters better than other groups after thawing in bull semen thus, it was concluded that 7-DHCLC could be used instead of CLC in bull semen cryopreservation. Keywords: Cholesterol 7-dehydrocholesterol, Cyclodextrin, Glycerol, Bull semen, Freeze-thawed Kolestrol ve 7-Dehidrokolestrolün Farklı Gliserol Oranları İle Boğa Sperması Dondurulmasına Etkisi ÖzBu çalışmanın amacı, 7-dehidrokolesterol yüklü siklodekstrin (7-DHCLC) ve kolesterol yüklü siklodekstrin (CLC) 'in farklı gliserol (G) oranları ilavesi ile boğa sperması dondurulması üzerindeki etkilerini değerlendirmektir. Üç adet boğaya ait sperma örnekleri toplandı, birleştirildi ve yedi gruba ayrıldı; biri kontrol (C) ile, diğer altı grup ise CLC veya 7-DHCLC (1.5 mg/120x10 6 ) ve farklı G oranlarını (%3, 6 ve 9) içeren standart Tris sulandırıcısı ile sulandırıldı. Sulandırılan spermalar 0.25 mL'lik payetlerde donduruldu. Çözdürme sonrası gruplar hareket karakteristikleri ve motilite, viabilite, akrozom bütünlüğü, mitokondriyal aktivasyon, fonksiyonel membran bütünlüğü ve anormal spermatozoa oranları yönünden değerlendirildi. En yüksek membran bütünlüğü, viabilite ve motilite %9 G ilavesi ile 7-DHCLC grubunda tespit edildi (P<0.001). En düşük progresif motilite %3 G gruplarında saptandı (P<0.001). %9 G içeren 7-DHCLC grubu, %3 G gruplarına kıyasla daha yüksek mitokondriyal aktivite belirlendi. Ayrıca, gruplar arasında akrozom bütünlüğü ve anormal spermatozoa oranları açısından istatistiksel bir fark gözlenmedi (P>0.05). Bu sonuçlara göre, %9 G ilave edilen 7-DHCLC grubunun, diğer gruplara kıyasla eritme sonrası bazı spermat...

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Section: Discussionsupporting

confidence: 79%

“…In some studies demonstrated that CLC treatment with semen affect sperm membrane potential and permeability [22] . Post-thaw sperm quality positively correlated with G level and CLC.…”

Section: Introductionmentioning

confidence: 99%

Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

̇İnanç¹,

Olğaç²,

Tekin³

et al. 2018

Kafkas Univ Vet Fak Derg

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“…cholesterol, one type of sterol, into plasma membranes prior to cryopreservation enhanced the functionality of sperm in several mammalian species (Pamornsakda et al, 2011;Tomas et al, 2011;Hussain et al, 2013). Despite the direct link between membrane sterol content and the impaired function of mammalian sperm, there has been no report exploring the change in sterol content in cryopreserved avian sperm.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken

et al. 2016

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Cryopreserved sperm undergoes serious damage which affects its fertilizing ability. Despite progress in understanding the nature of functional deterioration in mammalian sperm, little is known about the mechanism involved in the induction of functional damage in avian sperm. Cellular membranes are considered the primary site of cryodamage to sperm. Membrane rafts are specific membrane regions enriched in sterols, ganglioside G M1 , and functional proteins and they play important roles in the regulation of diverse functions exerted in mammalian sperm during fertilization. Several reports investigating cryopreservation-induced membrane changes in mammalian sperm have suggested that cryopreservation induces a compositional alteration of membrane rafts via a loss of membrane sterols, leading to impaired fertilizing ability. Recently, we demonstrated that membrane rafts are present in chicken sperm. Therefore, we investigated a possible mechanism for the induction of functional damage in cryopreserved chicken sperm, with particular attention to cryopreservation-induced compositional changes in membrane rafts. Sterol quantification showed that loss of sterols from sperm membranes occurred following cryopreservation. Biochemical analyses of detergent-insoluble membranes showed that the lipid and protein compositions of membrane rafts were altered dramatically by cryopreservation. To determine the physiological role of these changes, we examined external translocation of phosphatidylserine (PS), representing an early apoptotic change, and found that cryopreservation induced apoptotic changes in chicken sperm. Furthermore, methyl-β-cyclodextrin-induced loss of sterols from the plasma membranes stimulated PS translocation that was not accompanied with caspase-3 activation, which plays an important role downstream of the apoptotic cascade. Based on the results obtained in this study, we discuss a new mechanism for reduction of the fertilizing ability in avian sperm after cryopreservation.

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“…The addition of cholesterol-loaded cyclodextrins (CLC) to freezing extenders has been reported to increase the levels of cholesterol in the plasma membrane, which results in an augment in the stability of that membrane and thus in the post-thaw sperm survival and fertilizing ability (Tomás et al, 2011(Tomás et al, , 2013Blanch et al, 2012).…”

Section: Improvement Of Cryopreservation Extendersmentioning

confidence: 99%

State-of-the-art of boar sperm preservation in liquid and frozen state

Yeste

2017

Anim Reprod

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Pig breeding is mainly conducted through Artificial Insemination (AI) in Western and developing countries. Apart from requiring specific catheters and trained staff, preserving boar semen in proper conditions is needed to ensure high reproductive performances. Although, at present, boar sperm may be preserved in liquid (15-17ºC) or frozen states, more than 95% of AIs are conducted using liquid semen. The present work reviews the state-the-art of these two preservation technologies. Thus, the composition and types of extenders for liquid-stored semen are discussed, together with the specific requirements for boar sperm, which are stored at 15-17ºC. Commercial extenders for liquid semen are compared and the effects of storage on sperm quality are also summarised. In the second part of the manuscript, the main features of boar sperm cryopreservation are described and reference to cryodamage is also made. These cryoinjuries mainly affect sperm motility, membrane permeability and chromatin integrity. Furthermore, the individual variability in the sperm resilience to withstand cryopreservation procedures is reviewed and a brief summary about freezability markers is also included. Final sections briefly discuss the improvement of freezing extenders with additives, such as seminal plasma and antioxidants, and highlight the relevance of using a proper AI technique to avoid dramatic drops in reproductive performance.

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Treating boar sperm with cholesterol-loaded cyclodextrins widens the sperm osmotic tolerance limits and enhances the in vitro sperm fertilising ability

Cited by 45 publications

References 52 publications

Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken

State-of-the-art of boar sperm preservation in liquid and frozen state

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