Treatment of Pimpinella Alpina Molk Improve Oxidative stress and Inhibit Liver Cellular Apoptosis in Rats Following UVB Irradiation: Is there Any Correlation?

Abstract

Objective: The effect of Pimpinella alpina Molk (PaM) in improvement of oxidative stress has been established, but did not for cellular apoptosis. This investigation was conducted to evaluate the effect of PaM on GPx and XO activities, hepatocytes apoptosis and their correlation following UVB radiation. Methods: Forty male rats were assigned into 8 groups: Nor-G, Neg-7, Neg-15, PaM50-7, PaM100-7, PaM150-7, PaM100-15, and PaM150-15. UVB irradiation was given 10 minutes to each rat daily, blood and liver organ samples were taken in day 8 and day 16 to evaluate all variables. Results: Post Hoc statistical analysis showed that GPx activity in PaM150-15 was not significant lower compared to that of Nor-G, p > 0.05. Conversely, the activity of XO in PaM150-7 was not significant higher compared to that of Nor-G, p > 0.05. Expression of Bax and Caspase3 mRNA in PaM100-15 and PaM150 were comparable to that of Nor-G, p > 0.05. The strong negative correlation (-795, p < 0.01) was occur between GPx and XO. Similarly, there were strong negative correlations (-697 and -715, p < 0.001) between GPx and the expression of Bax and Caspase3 mRNA. Conclusion: PaM treatment with 100 -150 mg daily dose for 15 days capable of improving oxidative stress marked by increase in GPx activity and decrease in XO activity and inhibit apoptosis characterized by decrease in the expression of Bax and caspase3 mRNA. There were strong negative correlations between antioxidant activity and apoptosis on Sprague male rats after UVB irradiation.

Effect of Zamzam Water Extract of Curcuma zedoaria Treatment on Apoptosis, p53 and Bcl2 Protein Expression of T47D Cell Culture

“…This result implied that zamzam water extract of CZ treatment by the dose of 100 and 500 µl on T47D cell line was unable to trigger DNA damage largely and subsequently apoptosis. In the biological system, apoptosis is used to delineate the intrinsic cell suicide program, and morphologically is characterized by global cell shrinkage, cell blabbing, and chromatin condensation accompanied by nuclear and DNA fragmentation into specific fragment sizes form DNA ladder pattern 22 . Initiation of apoptosis by the cell is determined by gene activities, leading to cellular self-destruction through either intrinsic or the extrinsic or both pathways 23 .…”

Effect of Thyponium flageliforme in Combination with Curcuma zedoaria and Phyllanthus niruri in Expression of Protein Estrogen Receptor and Caspase-9 on Breast Cancer Mice

“…The result of the present study has also supported the theory that, apoptosis is a process triggered by death signal induction derives from both internal and external milieu, inducing mitochondrial membrane to release cytochrome-c. Cytochrome-c holoform along with apaf-1, procaspase-9, and Adenosintriphosphat (ATP) to form apoptosomedeath complex, that proteolitically convert procaspase-9 to caspase-9, and then activate caspase-7, caspase-6, caspase-3, and finally induce DNA fragmentation and cell death 25 . On the other hand, apoptosis can be prevented by Bcl-2 (a protein anti apoptosis) through releasing cytochrome-c and diablo/ Smac, an Inhibitor Apoptosis Protein (IAP) binding, originated from mitochondria.…”

Comparison of Bax and Caspase-3 Protein Expression in Liver Cells following UVB Irradiation for 7 days and Treatment of Pimpinella alpina Molk during 7 and 15 days

“…Pimpinella alpina Molk (PaM) treatment with 100-150 mg daily dose during 7 days have been proven capable of inhibiting liver apoptosis characterized by decrease in mRNA protein expression of Bax and Caspase-3 following ultraviolet B (UVB) light irradiation in rats. 1 Considering cell is able to denovo synthesis protein and other building blocks in order to regenerate their own self structure following damage from variety insults, 2-5 therefore the emerging question is, whether treatment with PaM in the same doses (100-150 mg daily) during 15 days will reduce apoptosis marked by Bax and Caspase-3 protein expression significantly compared to those of 7 days treatment following UVB irradiation. In addition, in this study, identification of Bax and Caspase-3 protein expression in liver cells will be assessed by immunohistochemical (IHC) staining method to confirm the previous result were measured by Rt-PCR method.…”

“…6,7 The most powerful and damaging effect of UVB on skin is mostly attributed to reactive oxygen species (ROS) particularly radical hydroxyl (OH • ). 1 Another ROS which is also produced in skin triggered by UVB are hydrogen peroxide (H 2 O 2 ) and oxygen superoxide (O 2 -) resulted from mitochondrial oxidative phosphorilation. 1,8 Furthermore, H 2 O 2 is rapidly circulated in systemic circulation and may be degraded to OH • through Fenton or Heber Weis reaction following UVB radiation.…”

“…1 Another ROS which is also produced in skin triggered by UVB are hydrogen peroxide (H 2 O 2 ) and oxygen superoxide (O 2 -) resulted from mitochondrial oxidative phosphorilation. 1,8 Furthermore, H 2 O 2 is rapidly circulated in systemic circulation and may be degraded to OH • through Fenton or Heber Weis reaction following UVB radiation. 9 Consequently, the cellular damages, DNA fragmentation, and apoptosis triggered by UVB occur not just in skin but also in liver, kidney, and other deeper cells.…”