Trehalose Accumulation in Wheat Plant Promotes Sucrose and Starch Biosynthesis

Background It has been previously shown that oligo-carrageenan (OC) kappa increases growth, photosynthesis and activities of enzymes involved in basal and secondary metabolisms in Eucalyptus globulus. However, it is not known whether OC kappa may induce the activation of TOR pathway and the increase in expression of genes encoding proteins involved in photosynthesis and enzymes of basal and secondary metabolisms. Results E. globulus trees were sprayed on leaves with water (control) or with OC kappa 1 mg mL − 1 , once a week, four times in total, and cultivated for 17 additional weeks (21 weeks in total). Treated trees showed a higher level of net photosynthesis than controls, beginning at week 3, a higher height, beginning at week 9, and those differences remained until week 21. In addition, treated trees showed an increase in the level of glucose beginning at week 1, trehalose at weeks 1–3, and in TOR-P level at week 1–2. On the other hand, transcripts encoding proteins involved in photosynthesis, and enzymes involved in glucose accumulation, C, N and S assimilation, and synthesis of secondary metabolites began at weeks 3–4 and with additional peaks at weeks 5–6, 8–11,13–14 and 17–19. Thus, OC kappa induced initial increases in glucose, trehalose and TOR-P levels that were followed by oscillatory increases in the level of transcripts coding for proteins involved in photosynthesis, and in basal and secondary metabolisms suggesting that initial increases in glucose, trehalose and TOR-P may trigger activation of gene expression. Conclusions The stimulation of growth induced by OC kappa in E. globulus trees is due, at least in part, to activation of TOR pathway and the increase in expression of genes encoding proteins involved in photosynthesis and enzymes of basal metabolism. Electronic supplementary material The online version of this article (10.1186/s12870-019-1858-z) contains supplementary material, which is available to authorized users.

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“…Quantification of trehalose was performed as described in [55]. Fresh leaves (0.1 g) were frozen in liquid nitrogen and homogenized in a mortar.…”

Section: Methodsmentioning

confidence: 99%

Oligo-carrageenan kappa increases glucose, trehalose and TOR-P and subsequently stimulates the expression of genes involved in photosynthesis, and basal and secondary metabolisms in Eucalyptus globulus

et al. 2019

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“…Earlier, it has been observed that accumulation of Tre in wheat plants promote biosynthesis of both sucrose and starch [34]. Further, the accumulation of Tre increased in wheat cultivation under drought stress [35].…”

Section: Starch Contentmentioning

confidence: 99%

Effect of Exogenous Trehalose in Protection of Cells and Their Membranes in Wheat (Triticum aestivum L.) Genotypes Under Heat Stress

Kaur

Thind

2018

CJAST

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Trehalose (Tre) a non-reducing disaccharide, formed of two alpha D-glucose molecules, is found in many organisms and is able to protect against a variety of environmental injuries and nutritional limitations. The information regarding its occurrence and role in higher plants is scanty. In the present study, effect of trehalose (@1 mM and 1.5 mM) under heat stress of 35±2°C (moderate) and 40±2°C (severe) for 4 and 8 hours was studied in wheat (Triticum aestivum L.) genotypes viz. HD2967, PBW175, C306, PBW343, PBW621 and PBW590. Membrane thermal stability (MTS) and cell viability were significantly decreased under heat stress of eight hours. Pretreatment with Tre improved MTS and cell viability of selected wheat genotypes under stress and the effect was more pronounced with higher concentration (1.5 mM). Plant growth and vigour were negatively affected by severe heat stress (40±2°C); the reduction was compensated partially by Tre application. Dry matter accumulation and starch content of all studied wheat genotypes decreased under the heat stress, the ameliorative effect was observed with the trehalose application that helped seedlings to sustain growth under heat stress.

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“…Interestingly, this result suggests that sucrose enhanced these processes through sucrose hydrolysis to glucose and fructose by the activity of invertase enzyme. According to several previous studies, glucose and fructose from sucrose hydrolysis by the cell wall invertase play a very important role in cell metabolism, signal molecules and regulators in the regulation of gene expression [14,[29][30][31][32][33][34][35][36]. Furthermore, the resultant sugars of sucrose hydrolysis must be transported to the cytoplasm by a monosaccharide transporter [37][38][39][40].…”

Section: Sucrose Promotes Pollen Germination and Pollen Tube Elongationmentioning

confidence: 99%

Pollen Germination and Pollen Tube Elongation of Tomato (Lycopersicum esculentum L.) Regulated by Cell Wall Invertase through Sucrose Hydrolysis

Astija¹

2017

JAST-A

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Sucrose is a sugar required for pollen germination and pollen tube elongation. It is little known on the regulation mechanism. As such, this research was conducted to reveal mechanism pathway of the sugar in regulating pollen germination and pollen tube elongation by cell wall invertase. The pollen grains, respectively originated from wild type and transgenic tomato plants, which had been silenced their inhibitor gene (INVINH1) of the cell wall invertase were used in this study. The pollen grains were cultured in media containing glucose, fructose or sucrose. Results of the study showed that percentage of the pollen germination derived from transgenic plants was much higher than that from wild type plants. Moreover, pollen tube elongation was longer in transgenic plants compared with wild type plants. Interestingly, these results were observed in medium containing sucrose only, but not in glucose and fructose. This result suggests that cell wall invertase activity regulates pollen germination and pollen tube elongation through sucrose hydrolysis.

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Trehalose Accumulation in Wheat Plant Promotes Sucrose and Starch Biosynthesis

Cited by 13 publications

References 48 publications

Oligo-carrageenan kappa increases glucose, trehalose and TOR-P and subsequently stimulates the expression of genes involved in photosynthesis, and basal and secondary metabolisms in Eucalyptus globulus

Oligo-carrageenan kappa increases glucose, trehalose and TOR-P and subsequently stimulates the expression of genes involved in photosynthesis, and basal and secondary metabolisms in Eucalyptus globulus

Effect of Exogenous Trehalose in Protection of Cells and Their Membranes in Wheat (Triticum aestivum L.) Genotypes Under Heat Stress

Pollen Germination and Pollen Tube Elongation of Tomato (Lycopersicum esculentum L.) Regulated by Cell Wall Invertase through Sucrose Hydrolysis

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