Trehalose and Tween 80 Improve the Stability of Marine Lysozyme During Freeze-Drying

Ji, Chunyan; Sun, Ming; Yu, Jia; Wang, Y.; Zheng, Yao; Wang, H.; Niu, Ruobing

doi:10.1080/13102818.2009.10817668

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…This may be due to the protecting effects of non-ionic surfactants such as polysorbate 80 against interfacial stresses that induced aggregation of CDNs during lyophilization. Moreover, binding of the hydrophobic segment of polysorbate 80 to the hydrophobic region of CDNs’ proteins may prevent protein self-association during lyophilization and storage [ 44 , 45 ]. Another potential mechanism is that polysorbate 80 preferentially coats exposed hydrophobic segments and thereby prevents the proteins from unfolding at the surface, thus maintaining their functional properties [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Lyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles

et al. 2021

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Recently, bioinspired cell-derived nanovesicles (CDNs) have gained much interest in the field of nanomedicine due to the preservation of biomolecular structure characteristics derived from their parent cells, which impart CDNs with unique properties in terms of binding and uptake by target cells and intrinsic biological activities. Although the production of CDNs can be easily and reproducibly achieved with any kind of cell culture, application of CDNs for therapeutic purposes has been greatly hampered by their physical and chemical instability during long-term storage in aqueous dispersion. In the present study, we conceived a lyophilization approach that would preserve critical characteristics regarding stability (vesicles’ size and protein content), structural integrity, and biological activity of CDNs for enabling long-term storage in freeze-dried form. Compared to the lyoprotectant sucrose, trehalose-lyoprotected CDNs showed significantly higher glass transition temperature and lower residual moisture content. As assessed by ATR-FTIR and far-UV circular dichroism, lyophilization in the presence of the lyoprotectant effectively maintained the secondary structure of cellular proteins. After reconstitution, lyoprotected CDNs were efficiently associated with HeLa cells, CT26 cells, and bone marrow-derived macrophages at a rate comparable to the freshly prepared CDNs. In vivo, both lyoprotected and freshly prepared CDNs, for the first time ever reported, targeted the injured heart, and exerted intrinsic cardioprotective effects within 24 h, attributable to the antioxidant capacity of CDNs in a myocardial ischemia/reperfusion injury animal model. Taken together, these results pave the way for further development of CDNs as cell-based therapeutics stabilized by lyophilization that enabled long-term storage while preserving their activity.

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Section: Resultsmentioning

confidence: 99%

Lyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles

et al. 2021

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“…In our system, both these hypotheses allow explaining the preservation of LYS native structure. [36][37][38][39] Alginate can interact with LYS molecules through hydrogen bonds, 40 exerting a thermodynamic protective effect during dehydration. Even HPMC could have a role because it can act as cryoprotectant.…”

Section: Discussionmentioning

confidence: 99%

Lysozyme Mucoadhesive Tablets Obtained by Freeze-Drying

Gennari

Sperandeo

Polissi

et al. 2019

Journal of Pharmaceutical Sciences

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“…However, trehalose alone had no protective effect during nebulization (data not shown), which made it less versatile than C 16 TreSuc . More interestingly, C 16 TreSuc was compared to PS80, recognized as a gold standard for protein stabilization, in liquid-form storage ( Warne, 2011 ), but also during nebulization ( Respaud et al, 2014 , Sécher et al, 2022 ) or lyophilization ( Ji et al, 2014 ). We studied the influence of C 16 TreSuc and the reference PS80 surfactants on antibody aggregation and antigen-binding over the whole lifecycle of the drug.…”

Section: Resultsmentioning

confidence: 99%

Novel trehalose-based excipients for stabilizing nebulized anti-SARS-CoV-2 antibody

Noverraz

Robin

Passemard

et al. 2023

International Journal of Pharmaceutics

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Trehalose and Tween 80 Improve the Stability of Marine Lysozyme During Freeze-Drying

Cited by 7 publications

References 14 publications

Lyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles

Lyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles

Lysozyme Mucoadhesive Tablets Obtained by Freeze-Drying

Novel trehalose-based excipients for stabilizing nebulized anti-SARS-CoV-2 antibody

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