Trehalose induced by reactive oxygen species relieved the radial growth defects of Pleurotus ostreatus under heat stress

Lei, Min; Wu, Xiangli; Huang, Chenyang; Qiu, Zhen; Wang, Lining; Zhang, Ruiying; Zhang, Jinxia

doi:10.1007/s00253-019-09834-8

Cited by 35 publications

(45 citation statements)

References 49 publications

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“…Only sucrose (with the peak change value of 1.914-fold under heat stress; multiple changes below were expressed in the same way) and mannitol (1.961-fold) increased with continued heat stress and decreased correspondingly when mycelia resumed growth (Figure 3), which may attribute to the extracon medium (methanol) does not extract high molecular weight compounds such as the polysaccharides. Thus, the experiments just detected the low molecular weight saccharides, and the results showed that heat stress had little effect on low molecular weight saccharides, even the trehalose, a disaccharide act as stress protectant to stabilize cell membranes and protect biological structures (Lei et al, 2019), has not changed significantly here.…”

Section: Dynamic Metabolites Changes In Enrichment Pathways Involvedmentioning

confidence: 84%

“…Heat treatment began on the sixth day at 40 • C (Lei et al, 2019) for 0-48 h, and after heat treated 48 h, the mycelia were recovered for 6 h at 28 • C. All treatments included controls at 28 • C. The samples were collected in tubes after heat treatment for 0, 6, 12, 24, 48 h (Wang, 2019), and 48 h + 6 h (means heat treated 48 h and then recovered 6 h) with seven repeats per group. The tubes were frozen in liquid nitrogen as soon as samples were collected and finally stored at −70 • C until use.…”

Section: Culture Conditions Heat Treatment and Sample Collectionmentioning

confidence: 99%

“…Acetic acid and acetoacetic acid, related to acetyl CoA, increased by 1.295fold and 1.731-fold, respectively (Figure 7). The significantly decreased sphinganine, sphingosine 1-phosphate and LysoPC under heat stress may indicate that the cell membrane is damaged by heat stress, combining that the malondialdehyde (MDA) level significantly increased under heat stress (Lei et al, 2019).…”

Section: Dynamic Metabolites Changes In Enrichment Pathways Involvedmentioning

confidence: 99%

“…Pleurotus ostreatus has economic and ecological values and medicinal properties and has thus been intensely studied and cultivated in many different areas of the world (Sánchez, 2010). However, the cultivation of P. ostreatus is still dependent on the natural environmental temperature in China and many other developing countries (Lei et al, 2019). Thus, high temperature is the most adverse stress in the cultivation of P. ostreatus.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Yan

Zhao

et al. 2020

Front. Microbiol.

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Heat stress seriously threatens the growth of Pleurotus ostreatus. Various studies have been performed to study the resistance of P. ostreatus to heat stress. Here, the metabolome was evaluated to determine the response of P. ostreatus mycelia to heat stress at different times (6, 12, 24, 48 h). More than 70 differential metabolites were detected and enriched in their metabolic pathways. Dynamic metabolites changes in enrichment pathways under heat stress showed that heat stress enhanced the degradation of unsaturated fatty acids and nucleotides, increased the content of amino acids and vitamins, and accelerated glycolysis and the tricarboxylic acid cycle in P. ostreatus. The time course changes of P. ostreatus metabolites under continuous heat stress demonstrated that amino acids continuously changed with heat stress, nucleotides clearly changed with heat stress at 12 and 48 h, and lipids exhibited an increasing trend with prolonged heat stress, while few types saccharides and vitamins changed under heat stress. Additionally, heat-treated P. ostreatus produced salicylic acid and other stress-resistant substances that were reported in plants. This study first reported the metabolites changes in P. ostreatus mycelia during 48 h of heat stress. The metabolic pathways and substances that changed with heat stress in this research will aid future studies on the resistance of P. ostreatus and other edible fungi to heat stress.

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Section: Dynamic Metabolites Changes In Enrichment Pathways Involvedmentioning

confidence: 84%

Section: Culture Conditions Heat Treatment and Sample Collectionmentioning

confidence: 99%

Section: Dynamic Metabolites Changes In Enrichment Pathways Involvedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Yan

Zhao

et al. 2020

Front. Microbiol.

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“…RNA extraction, reverse transcription, and qPCR. The levels of specific mRNAs expressed by the WT and transformed strains were assessed using qPCR according to methods in a previous study (3). A fusion fragment containing the gpd promoter and aco gene was amplified using the primer pair gpdϩaco-F and gpdϩaco-R (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Hou

Zhao

Huang

et al. 2020

Appl Environ Microbiol

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Pleurotus ostreatus is widely cultivated in China. However, its cultivation is strongly affected by seasonal temperature changes, especially the high temperatures of summer. Nitric oxide (NO) was previously reported to alleviate oxidative damage to mycelia by regulating trehalose. In this study, we found that NO alleviated oxidative damage to P. ostreatus mycelia by inhibiting the protein and gene expression of aconitase (ACO), and additional studies found that the overexpression and interference of aco could affect the content of citric acid (CA). Furthermore, the addition of exogenous CA can induce alternative oxidase (aox) gene expression under heat stress, reduce the content of H 2 O 2 in mycelium, and consequently protect the mycelia under heat stress. An additional analysis focused on the function of the aox gene in the heat stress response of mycelia. The results show that the colony diameter of the aox overexpression (OE-aox) strains was significantly larger than that of the wild-type (WT) strain under heat stress (32°C). In addition, the mycelia of OEaox strains showed significantly enhanced tolerance to H 2 O 2 . In conclusion, this study demonstrates that NO can affect CA accumulation by regulating aco gene and ACO protein expression and that CA can induce aox gene expression and thereby be a response to heat stress. IMPORTANCE Heat stress is one of the abiotic stresses that affect the growth and development of edible fungi. Our previous study found that exogenous NO had a protective effect on mycelia under heat stress. However, its regulatory mechanism had not been elucidated. In this study, we found that NO altered the respiratory pathway of mycelia under heat stress by regulating aco. The results have enhanced our understanding of NO signaling pathways in P. ostreatus.

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Research Progress on Edible Fungi Genetic System

Shi

Zhu

et al. 2022

Advances in Biochemical Engineering/Biotechnology

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Trehalose induced by reactive oxygen species relieved the radial growth defects of Pleurotus ostreatus under heat stress

Cited by 35 publications

References 49 publications

Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Research Progress on Edible Fungi Genetic System

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