TREK Channel Pore Probed by Cysteine Scanning Mutagenesis and Structural Modelling

Abstract

more direct role of G i/o proteins in TRPC4/C5 activation is supported by the demonstration that Ga i and Ga o subunits physically bind to the C-terminus of TRPC4. We suggest that a concerted action of G q/11 -and G i/o -mediated signaling pathways is required for the full activation of TRPC4/C5, making these channels coincident detectors of multiple environmental cues. We have tested the co-dependence of native TRPC4/C5-like currents in smooth muscle cells, endothelial cells and neurons.