Trends in Laboratory Diagnostic Methods in Periodontology

Bolerázska, Beáta; Mareková, Mária; Markovská, N

doi:10.14712/18059694.2016.47

Cited by 16 publications

(14 citation statements)

References 49 publications

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“…However the nature and source of inflammation is unclear, but many studies have proven that periodontal therapy resolves the periodontal as well as systemic inflammation 171819. Recent studies demonstrated that, traditional clinical measures of human periodontal diseases are not designating of ongoing the future disease progression 20. Determination of the presence of inflammatory products found in GCF or saliva may be of value in evaluating both periodontal disease status and the outcome of therapy 21.…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Salivary Neopterin Levels and Its Effects to Periodontal Therapy in Pre- and Post-Menopausal Women

et al. 2017

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ObjectivesNeopterin is a valuable diagnostic biomarker, which is elevated in inflammatory conditions like periodontitis, that is characterized by destruction of the supporting structures of the teeth. Among the biomarkers, neopterin occurs in body fluids, and acts as a diagnostic marker for present and future disease activity.MethodsThirty female subjects with chronic periodontitis, mean age 50 years (40-60 years) were included in this study. Depending upon their menstrual history, subjects were categorized into two groups of fifteen each. Group I 15 pre-menopausal women, and Group II 15 post-menopausal women. Saliva was collected, and neopterin levels were assessed by enzyme-linked immunosorbent assay in both the groups, at base line and after three months of nonsurgical periodontal therapy (NSPT). Periodontal parameters like pocket probing depth (PD) and Russell's periodontal disease index (PDI) were assessed before treatment as well as after three months of scaling and root planning.ResultsIntra group analysis showed significant markdown in the mean values of all the parameters from baseline to three months (P < 0.001), for all patients. The intergroup comparison, from baseline to 3 months also showed no significant change in PD and PDI values, but there was a statistically significant difference in the salivary neopterin levels (P = 0.04).ConclusionsNeopterin levels were found to be reduced in three months after NSPT in both the groups, suggesting that the NSPT is the gold standard therapy, and also that neopterin levels in saliva can be used as an indicator to identify periodontal inflammation and destruction.

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Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Salivary Neopterin Levels and Its Effects to Periodontal Therapy in Pre- and Post-Menopausal Women

et al. 2017

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“…Tissue engineering is a novel approach that is emerging as an alternative to regenerating tissues lost or damage by trauma, congenital anomalies, or chronic diseases 1,2) . In dentistry, the main concern is to attempt to regenerate lost tissue caused by periodontal disease (PD) [3][4][5] . PD is considered one of the most destructive dental pathologies and as a chronic inflammatory disorder.…”

Section: Introductionmentioning

confidence: 99%

Influence of diameter of fiber membrane scaffolds on the biocompatibility of hPDL mesenchymal stromal cells

et al. 2018

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This study evaluated the influence in the biocompatibility of human periodontal ligament (hPDL) mesenchymal stromal cell onto poly lactic-acid (PLA) films and PLA fiber membrane. Fiber scaffold was prepared via air jet spinning (AJS) from PLA solutions (6, 7, and 10%) and analyzed using SEM, AFM and FTIR. Biocompatibility was evaluated by adhesion, proliferation and cell-material interaction. PLA film exhibited a smooth and homogenously surface topography in comparison with random orientation of PLA fiber with roughness structure where diameter size depends on PLA solution. Moreover, cell adhesion; proliferation and cell-material interaction has the best respond on random orientation nanofiber of 10, followed by 7, and 6% of PLA in comparison with PLA films. It could be concluded that AJS is an attractive alternative technique for manufacture fiber scaffolds with a tunable random orientation geometry of fibers that allow to produce interconnected porous formed by nanometric fiber diameter structures that could be a potential scaffold for periodontal tissue engineering applications.

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“…However, there are some limitations associated with this procedure. Indeed, cultivation cannot detect non-viable bacteria, as well as, hardly cultivated bacteria important in the etiology of periodontitis, such as T. denticola 6,7 . Nowadays, alternative nucleic acid-based methods, more sensitive than bacterial culture, have been used to detect and quantify pathogens present in oral cavity 5,6,8 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of two different methods for detecting periodontal pathogenic bacteria

et al. 2017

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Aim: To perform a comparative analysis between two methods for detecting Porphyromonas gingivalis, Tannerella forsythia and Porphyromonas endodontalis in periodontal plaque samples. Methods: The study sample consisted of twenty systemically healthy patients showing generalized chronic periodontitis. The subgingival samples for microbiological analysis were collected before (baseline) and 60 days after a basic periodontal therapy from 30 non-adjacent affected sites (Probing Depth (PD): 5-7 mm, Clinical Attachment Loss (CAL) ≥ 5 mm, positive for Bleeding on Probing (BOP)). Microbiological analysis was performed by PCR and qPCR. To allow a comparative analysis between both methods, qPCR was divided in three different scores (score 2: presence of more than 100 bacteria; score 1: presence of 10-100 bacteria, and score 0: absence of bacteria), in accordance to DNA quantity, while for PCR two scores were assigned: presence or absence of bacteria. Results: qPCR demonstrated higher sensitivity in the detection of these pathogens compared with PCR when scores 1 and 2 were considered positive. However, when only score 2 was considered positive, PCR and qPCR showed better agreement. Conclusions: qPCR demonstrated higher sensitivity than conventional PCR for detection of low numbers of microorganisms and can be useful for the quantification of periodontopathogens.

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Trends in Laboratory Diagnostic Methods in Periodontology

Cited by 16 publications

References 49 publications

Comparative Evaluation of Salivary Neopterin Levels and Its Effects to Periodontal Therapy in Pre- and Post-Menopausal Women

Comparative Evaluation of Salivary Neopterin Levels and Its Effects to Periodontal Therapy in Pre- and Post-Menopausal Women

Influence of diameter of fiber membrane scaffolds on the biocompatibility of hPDL mesenchymal stromal cells

Comparison of two different methods for detecting periodontal pathogenic bacteria

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