??-tricalcium Phosphate Delivery System for Bone Morphogenetic Protein

Urist, Marshall R.; Lietze, Arthur; Dawson, Edgar G.

doi:10.1097/00003086-198407000-00042

Cited by 212 publications

(126 citation statements)

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“…Although BMP can induce bone formation when added as a solution, and not bound to a carrier (120,121), the dose needed to induce endochondral bone formation can be greatly reduced when BMP is combined with an appropriate carrier (122). Collagen carriers, prepared by complete demineralization of bovine trabecular or cortical bone particles, have been shown to meet these requirements in animals (123,124).…”

Section: Role Of Bmp Carriers In Bone Inductionmentioning

confidence: 99%

Bone morphogenetic proteins in human bone regeneration

Groeneveld

Burgér

2000

European Journal of Endocrinology

358

217

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Recently, the first clinical reports on bone regeneration by two recombinant human bone morphogenetic proteins (rhBMPs), BMP-2 and BMP-7 (also named osteogenic protein-1, OP-1) have been published (1-4) . Although both BMPs were able to support bone regeneration, a significant variation in individual response was observed with both proteins. Animal studies and laboratory experiments reveal a number of conditions that influence the osteoinductivity of BMP, such as BMP concentration, carrier properties and influence of local and systemic growth factors and hormones. In this paper, these studies and the clinical reports are reviewed, and the conditions that modulate the BMP-dependent osteoinduction are discussed. The information may provide clues as to how the performance of recombinant human BMP as bone-graft substitute in humans can be improved. European Journal of Endocrinology 142 9-21 BMP and demineralized bone matrixThe history of bone morphogenetic proteins (BMPs) began with the observation that demineralized bone matrix (DBM) is able to induce ectopic bone formation in subcutaneous and intramuscular pockets in rodents (5, 6). This bone induction process has been studied extensively (7-13). Histological and biochemical analyses showed that cartilage appears 5-10 days after implantation of active DBM (8). This cartilage mineralizes by day 7-14 and is subsequently replaced by bone (7-9). After 21 days, haematopoietic bone marrow formation can be observed (12). These cellular events observed after DBM implantation mimic embryonic bone development and normal fracture repair (11). As DBM-related bone formation was observed to occur at ectopic sites, it was assumed that pluripotent mesenchymal cells are attracted to the site of implantation. Isolation of the bone-inducing substance revealed that certain proteins were responsible, which were termed bone morphogenetic proteins (BMPs) or osteogenetic proteins (OPs).The use of DBM in treating bone defects has proven beneficial for bone regeneration both in animals and in humans (14-17). DBM has become widely accepted as a bone-graft substitute in clinical practice (18-22), but its bone inductive capacity has been questioned (23, 24). In several studies, including our own, histology revealed that new bone was generated by osteoconduction rather than osteoinduction (25-27) -that is, bone regeneration occurred by growth of existing host bone on the DBM granules, which acted as a scaffold, rather than by de novo differentiation of bone, independent of pre-existing bone. Lack of inductive properties of DBM may be related to the procedures of production of commercially available DBM, as preservation of osteoinductive activity can be affected by the processing (28-30) or sterilization procedures (31). It is also possible that DBM of human origin, which is preferred for use in clinical practice, is less osteoinductive than DBM derived from animals, which is commonly used in animal studies. Several studies show that DBM from long-lived species such as baboon and human...

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Section: Role Of Bmp Carriers In Bone Inductionmentioning

confidence: 99%

Bone morphogenetic proteins in human bone regeneration

Groeneveld

Burgér

2000

European Journal of Endocrinology

358

217

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“…In this study, we used covalent cross-linked alginic acid sheet for transplantation as a carrier. Now, hydroxyapatite 42) , calcium phosphate 43) , polylactic acid and polyglycolic acid [44][45][46][47] have been investigated as a carrier, and these carriers had been proven its efficacy. We regarded that the alginic acid sheet have weak ability to induce hard tissue formation by itself ( Fig.…”

Section: Discussionmentioning

confidence: 99%

The possibility of genistein as a new direct pulp capping agent

et al. 2013

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Genistein, kind of soy isoflavones, is well-known as natural ingredients and consumed as health foods and supplements. They are expected to improve renal function. They have high-affinity to estrogen receptor β expressed predominantly in bone tissue, they prevent osteoporosis specifically and safely. We examined whether genistein can be a new direct capping agent. In this study, we examined the effect of genistein for the proliferation and differentiation of rat dental pulp cells in vitro and the ability of tertiary dentin formation in vivo. As a result, rat dental pulp cells with genistein were increased activity of ALPase and showed alizarin red positive-staining. Calcification-related genes expression has been confirmed by the addition of genistein. From in vivo study, high quality of tertiary dentin formation and minor pulp reaction were observed. From these findings, it was suggested that genistein may be useful agent for direct pulp capping.

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“…Implantation of BMP in a thigh muscle pouch of the mouse consistently induces new cartilage formation by the 7th day, woven bone by the 14th day, and lamellar bone by the 21st day (Urist et al 1984). But BMP extracted from bone matrix by chemical methods is limited in amount.…”

Section: Discussionmentioning

confidence: 99%

“…Some researchers (Sampath et al 1983, Urist et al 1984 found that BMP was present not only in the bone matrix of rats, pigs and humans, but also in the dentin of rabbits and humans. Yang and Yan (1990) demonstrated that BMP might be found in human tooth anlages such as human predentin, and cells of the outer and inner enamel epithelium.…”

Section: Discussionmentioning

confidence: 99%