Trichoderma Asperellum, an inoculant for production of steviol glycoside in Stevia Rebaudiana bertoni plants micropropagated in a temporary immersion  bioreactor

Villamarín-Gallegos, D.; Oviedo-Pereira, Dumas G.; Evangelista‐Lozano, Silvia; Sepúlveda-Jiménez, Gabriela; Molina‐Torres, Jorge; Rodrı́guez-Monroy, Mario

doi:10.24275/rmiq/bio947

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…Stevia (Stev) based sweeteners are natural, and highly acceptable in the market. These compounds are extracted from the plant Stevia rebaudiana, and the compounds of interest are known as steviol glycosides (Carocho et al, 2017;Villamarín-Gallegos et al, 2020). Also, Stev is reported to have beneficial effects linked to adjuvant metabolic syndrome diseases such as type 2 diabetes (Philippaert et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Physicochemical properties and sensory acceptability of sugar free dark chocolate formulations added with probiotics

Gómez-Fernández¹,

Faccinetto-Beltrán²,

Orozco-Sánchez³

et al. 2021

RMIQ

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Section: Introductionmentioning

confidence: 99%

Physicochemical properties and sensory acceptability of sugar free dark chocolate formulations added with probiotics

Gómez-Fernández¹,

Faccinetto-Beltrán²,

Orozco-Sánchez³

et al. 2021

RMIQ

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“…This study showed that biotization with endophytes on M. spicata microplants was effective, reducing the disease incidence to 8% for T. asperellum and 10% for B. subtilis , making it an alternative to conventional soil inoculation methods. According to [ 24 , 25 ], this method allows a better and faster interaction with the root system and a higher growth induction. It has been shown that although endophytes live in close association with plants, they need to overcome defense barriers such as specialized plant metabolites [ 26 ]; so, these microorganisms need to produce enzymes that break them down and can colonize tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Endophytic bacteria, such as B. subtilis , can protect plants by producing antibiotics that inhibit pathogen development and promote induced systemic resistance (ISR) [ 31 ]. Inoculation of endophyte microorganisms during micropropagation is an alternative that can improve crop quality and increase the content of specialized metabolites such as flavonoids and phenolic compounds in general [ 25 , 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Biotization of Endophytes Trichoderma asperellum and Bacillus subtilis in Mentha spicata Microplants to Promote Growth, Pathogen Tolerance and Specialized Plant Metabolites

Castro-Restrepo

Dominguez

Gaviria-Gutiérrez

et al. 2022

Plants

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In the present study, the effect of biotization of Mentha spicata microplants with Trichoderma asperellum and Bacillus subtilis on growth, Rhizoctonia sp., incidence, and specialized metabolites content was evaluated. Analyses of root tissues of the microplants showed 100% endophytism with both microorganisms. During the acclimatization phase, plants with the endophytes T. asperellum and B. subtilis had a survival rate of 95% and 93%, respectively, compared to 75% for control plants. Then, under greenhouse conditions, a trial was carried out with biotized plants with or without Rhizoctonia sp. inoculation, plants inoculated with Rhizoctonia sp., and endophyte- and pathogen-free control. Biotized plants with the endophytes showed higher dry biomass and the incidence of Rhizoctonia was lower (8% for T. asperellum and 10% for B. subtilis) compared to plants inoculated with the pathogen (82%). In addition, plants with T. asperellum had the highest contents of total polyphenols (280 GAE/100 mg sample) and rosmarinic acid (28 mg RA/100 g sample). Thus, this study shows the potential of the technique of using the endophytes T. asperellum and B. subtilis on M. spicata microplants to improve plant survival and growth, decrease the incidence of Rhizoctonia sp., and improve the contents of specialized metabolites, which can contribute to the sustainable management of this crop.

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“…The supernatant was recovered, placed in Eppendorf tubes, and stored at −4 °C until the analysis by HPTLC (CAMAG, Switzerland). The quantification of SGs was based on the methodology reported by Bladt & Zgainski (1996) and Morlock et al (2014) , and described recently by Villamarin-Gallegos et al (2020) . Stevioside and rebaudioside A concentration were expressed in mg g DW −1 .…”

Section: Methodsmentioning

confidence: 99%

Photosynthetic performance and stevioside concentration are improved by the arbuscular mycorrhizal symbiosis in Stevia rebaudiana under different phosphate concentrations

Sarmiento-López

López-Meyer

Sepúlveda-Jiménez

et al. 2020

PeerJ

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In plants, phosphorus (P) uptake occurs via arbuscular mycorrhizal (AM) symbiosis and through plant roots. The phosphate concentration is known to affect colonization by AM fungi, and the effect depends on the plant species. Stevia rebaudiana plants are valuable sources of sweetener compounds called steviol glycosides (SGs), and the principal components of SGs are stevioside and rebaudioside A. However, a detailed analysis describing the effect of the phosphate concentration on the colonization of AM fungi in the roots and the relationship of these factors to the accumulation of SGs and photochemical performance has not been performed; such an analysis was the aim of this study. The results indicated that low phosphate concentrations (20 and 200 µM KH2PO4) induced a high percentage of colonization by Rhizophagus irregularis in the roots of S. rebaudiana, while high phosphate concentrations (500 and 1,000 µM KH2PO4) reduced colonization. The morphology of the colonization structure is a typical Arum-type mycorrhiza, and a mycorrhiza-specific phosphate transporter was identified. Colonization with low phosphate concentrations improved plant growth, chlorophyll and carotenoid concentration, and photochemical performance. The transcription of the genes that encode kaurene oxidase and glucosyltransferase (UGT74G1) was upregulated in colonized plants at 200 µM KH2PO4, which was consistent with the observed patterns of stevioside accumulation. In contrast, at 200 µM KH2PO4, the transcription of UGT76G1 and the accumulation of rebaudioside A were higher in noncolonized plants than in colonized plants. These results indicate that a low phosphate concentration improves mycorrhizal colonization and modulates the stevioside and rebaudioside A concentration by regulating the transcription of the genes that encode kaurene oxidase and glucosyltransferases, which are involved in stevioside and rebaudioside A synthesis in S. rebaudiana.

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Trichoderma Asperellum, an inoculant for production of steviol glycoside in Stevia Rebaudiana bertoni plants micropropagated in a temporary immersion bioreactor

Abstract: Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)

Cited by 12 publications

References 21 publications

Physicochemical properties and sensory acceptability of sugar free dark chocolate formulations added with probiotics

Physicochemical properties and sensory acceptability of sugar free dark chocolate formulations added with probiotics

Biotization of Endophytes Trichoderma asperellum and Bacillus subtilis in Mentha spicata Microplants to Promote Growth, Pathogen Tolerance and Specialized Plant Metabolites

Photosynthetic performance and stevioside concentration are improved by the arbuscular mycorrhizal symbiosis in Stevia rebaudiana under different phosphate concentrations

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