Trichosporon

Reiser, Jakob; Ochsner, Urs A.; Kälin, Markus; Glumoff, Virpi; Fiechter, Armin

doi:10.1007/978-3-642-79856-6_13

Cited by 5 publications

(1 citation statement)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…have the ability to use methanol as a sole carbon source (11). Adaptation to growth on methanol is associated with induction of alcohol oxidase (AOX) (also referred to as methanol oxidase [MOX]), which is virtually absent in glucose-grown cells (12), but can account for more than 30% of the cell protein in methanol-grown cells (13). Among the many Pichia species, P. pastoris is the most common for foreign protein expression (14,15).…”

Section: Introductionmentioning

confidence: 99%

Secretion of human interleukin-2 fused with green fluorescent protein in recombinatn pichia pastoris

Joon

Dalal

Bentley

2005

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Methylotrophic yeast Pichia pastoris is convenient for the expression of eukaryotic foreign proteins owing to its potential for posttranslational modifications, protein folding, and facile culturing. In this work, human interleukin (hIL)-2 was successfully produced as a secreted fusion form in recombinant P. pastoris. By employing green fluorescent protein (GFP) as a monitoring fusion partner, clear identification of fusion protein expression and quantification of intracellular hIL-2 were possible even though there was no correlation between culture supernatant fluorescence and secreted hIL-2 owing to high media interference. Importantly, by the addition of casamino acids in basal medium, we were able to enhance threefold amount of secreted hIL-2, which was present both as a fusion and as a clipped fragment.

show abstract

Section: Introductionmentioning

confidence: 99%

Secretion of human interleukin-2 fused with green fluorescent protein in recombinatn pichia pastoris

Joon

Dalal

Bentley

2005

Appl Biochem Biotechnol

View full text Add to dashboard Cite

show abstract

Basidiomycetous Yeasts

Fell

Boekhout

Fonseca

et al. 2001

Systematics and Evolution

View full text Add to dashboard Cite

Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production

Sanya

Onésime

Passoth

et al. 2021

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Strains of the yeast genus Blastobotrys (subphylum Saccharomycotina) represent a valuable biotechnological resource for basic biochemistry research, single-cell protein, and heterologous protein production processes. Species of this genus are dimorphic, non-pathogenic, thermotolerant, and can assimilate a variety of hydrophilic and hydrophobic substrates. These can constitute a single-cell oil platform in an emerging bio-based economy as oleaginous traits have been discovered recently. However, the regulatory network of lipogenesis in these yeasts is poorly understood. To keep pace with the growing market demands for lipidderived products, it is critical to understand the lipid biosynthesis in these unconventional yeasts to pinpoint what governs the preferential channelling of carbon flux into lipids instead of the competing pathways. This review summarizes information relevant to the regulation of lipid metabolic pathways and prospects of metabolic engineering in Blastobotrys yeasts for their application in food, feed, and beyond, particularly for fatty acid-based fuels and oleochemicals. Key points• The production of biolipids by heterotrophic yeasts is reviewed. • Summary of information concerning lipid metabolism regulation is highlighted.• Special focus on the importance of diacylglycerol acyltransferases encoding genes in improving lipid production is made.

show abstract

Trichosporon

Cited by 5 publications

References 68 publications

Secretion of human interleukin-2 fused with green fluorescent protein in recombinatn pichia pastoris

Secretion of human interleukin-2 fused with green fluorescent protein in recombinatn pichia pastoris

Basidiomycetous Yeasts

Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production

Contact Info

Product

Resources

About