Trifolium alexandrinum × T. resupinatum – Interspecific Hybrids Developed through Embryo Rescue

Kaushal, Pankaj; Malaviya, D. R.; Roy, A. K.; Kumar, Brijesh; Tiwari, Aparna

doi:10.1007/s11240-005-4442-1

Cited by 26 publications

(22 citation statements)

References 19 publications

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“…This corresponds to the results of Isobe et al (2001), who observed different leaf morphologies between BC 4 plants and T. pratense in the same cross combination in comparison with the parental species. The intermediate morphological appearance of interspecific hybrids was also observed in other species combinations, such as T. alexandrinum × T. apertum , T. alexandrinum × T. constantinopolitanum and T. alexandrinum × T. resupinatum (Kaushal et al 2005). A few hybrids of T. alexandrinum × T. apertum also showed better growth than either parent .…”

Section: Discussionsupporting

confidence: 52%

Variation of morphological and agronomic traits in hybrids of Trifolium pratense × T. medium and a comparison with the parental species

Jakešová¹,

Řepková²,

Hampel³

et al. 2011

CZECH J GENET PLANT

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Trifolium pratense L. is a high-yielding and high-quality fodder crop. However, it shows low persistence, which may be overcome by hybridisation with species that produce rhizomes. Hybrids between T. pratense L. and T. medium L. were previously obtained by embryo rescue. The aim of this study was to evaluate 16 characteristics (stem weight, stem length, internode number, length/width ratio of a central leaflet of the trifoliate leaf on the 4 th internode, stem thickness on the 4 th internode, average leaf area of the trifoliate leaf, weight of dry plant, was significantly higher in the hybrids compared to both parental species, which could have a positive impact on the yield. Short rhizomes were observed in the hybrids after the harvest of plants in the second harvest year. The stability of this trait and its impact on the perenniality of the hybrids will be investigated in the next years.Keywords: interspecific hybrids; morphological traits; persistence; red clover; rhizomes; zigzag cloverThe red clover (Trifolium pratense L.) is a widespread forage crop in the Czech Republic as well as in the rest of Europe. It is a high-yielding and high-quality fodder crop from the aspect of both nutrient content and ensilage suitability. Red clover has a high content of proteins, water-soluble carbohydrates, tannins, polyphenol oxidase and polyunsaturated fatty acids.Clover breeding is aimed at the production of fresh and dry matter that is of high quality combined with satisfactory seed yields. Polyploidy induction (tetraploidy 2n = 4x = 28) in red clover is useful for obtaining varieties with higher fodder production compared to diploids because autoploidy increases the cell size, especially in meristematic tissues, and the autoploids usually

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Section: Discussionsupporting

confidence: 52%

Variation of morphological and agronomic traits in hybrids of Trifolium pratense × T. medium and a comparison with the parental species

Jakešová¹,

Řepková²,

Hampel³

et al. 2011

CZECH J GENET PLANT

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“…Embryo culture technique has been successfully used to obtain interspecific hybrids in crops such as Trifolium and tomato. This method offers the chance to rescue plantlets from underdeveloped embryos by culturing on a suitably nutritional medium (Evans, 1962;Katznelson, 1971;Segeren et al, 1993;Kaushal et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

Jutamas

Wei²,

Lee³

et al. 2013

Genet. Mol. Res.

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ABSTRACT.Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them were confirmed to be true interspecific hybrids. Eighteen and 14 bands from 7 RAPD and 14 ISSR primers, respectively, were polymorphic and present in all five hybrid seedlings and their parents. The morphological characteristics of leaf margin, inflorescence type and spine positions of the five seedlings were intermediate to the parents. These interspecific hybrids had low pollen viability, probably due to abnormal meiosis.

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“…Williams (1978) noted that only 30 embryos from many hundred pollinated flowers were large enough for dissection and only 6 showed any growth in culture. In other research on hybridization between Trifolium alexandrinum and T. resupinatum, out of 556 crossing attempts, a total of 26 ovules were recovered (Malaviya et al, 2004a;Kaushal et al, 2005). In our study, after nearly 4-5 months of culturing in vitro, the remaining 53 were transferred to vermiculite.…”

Section: Resultsmentioning

confidence: 50%

Caucasian clover (Trifolium ambiguum Bieb.) × white clover (T. repens L.) – interspecific hybrids developed through tissue culture

Huang

Wang

2017

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Caucasian clover (Trifolium ambiguumBieb ABSTRACTIn order to create new forage variety, caucasian clover (Trifolium ambiguum Bieb.) with strong tolerance to drought and cold introduced from New Zealand as the female parent was hybridized with a white clover (T. repens L.) collected from northeast China, which has strong nitrogen fixation capacity. Hybrid embryos of caucasian clover×white clover were successfully raised using embryo rescue. 13.8% of these embryos grew to maturity. The successfully grown plants were transferred to sterilized vermiculite after 5-6 weeks and then to field after 3-4 months. The hybrid plant showed intermediate morphological features and a chromosomal number of 2n=5X=40. In order to obtain more plants for backcross, a tissue culture system was established for the hybrids, using MS+0.1mg l -1 2, 4-D+2mg l -1 6-BA, MS +0.5mg l -1 NAA+1mg l -1 6-BA+1mg l -1 KT and 1/2MS as the medium for bud-induction, differentiation and root-induction respectively. 80% of the cultured plantlets survived these growing conditions. Hybridity of the plant was also confirmed by ISSR markers using 16 primers.

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Trifolium alexandrinum × T. resupinatum – Interspecific Hybrids Developed through Embryo Rescue

Cited by 26 publications

References 19 publications

Variation of morphological and agronomic traits in hybrids of Trifolium pratense × T. medium and a comparison with the parental species

Variation of morphological and agronomic traits in hybrids of Trifolium pratense × T. medium and a comparison with the parental species

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

Caucasian clover (Trifolium ambiguum Bieb.) × white clover (T. repens L.) – interspecific hybrids developed through tissue culture

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