TRIM6 interacts with c-Myc and maintains pluripotency of mouse embryonal stem cells

Abstract

SummaryThe proto-oncogene product Myc is a master regulator of cell proliferation through its specific binding to the E-box motif in genomic DNA. It has been reported that Myc has an important role in the proliferation and maintenance of the pluripotency of embryonic stem (ES) cells and that the transcriptional activity of Myc is regulated by several post-translational modifications, including ubiquitination. In this study, we showed that tripartite motif containing 6 (TRIM6), one of the TRIM family ubiquitin ligases, was selectively expressed in ES cells and interacted with Myc followed by attenuation of the transcriptional activity of Myc. Knockdown of TRIM6 in ES cells enhanced the transcriptional activity of Myc and repressed expression of NANOG, resulting in the promotion of ES cell differentiation. These findings indicate that TRIM6 regulates the transcriptional activity of Myc during the maintenance of ES cell pluripotency, suggesting that TRIM6 functions as a novel regulator for Myc-mediated transcription in ES cells.

The effects of social connections on self-rated physical and mental health among internal migrant and local adolescents in Shanghai, China

“…Although we did not find a significant difference in student-teacher relations between migrant and native adolescents, local boys reported the lowest scores in teacher relations. A plausible explanation is that migrants and girls are generally considered more likely to obey rules, and respect teachers compared with local students and boys [36]. …”

“…Results support the discriminant validity of FACES-II for Chinese families. Peer association, and teacher-student relations were assessed by 6 and 5 items, respectively, from the School Adaption Scale [36], a 27-item scale that assesses student adaption in school life contexts. The 11 selected items were determined by factor analysis of the component items that reflect connection with peers and teachers.…”

Precision autophagy directed by receptor regulators – emerging examples within the TRIM family

“…TRIMs are involved in a variety of seemingly disparate cellular processes (Table S1); these include cell survival and death (Grignani et al, 1993), cell cycle and differentiation (Wang et al, 1998), metabolic states (Skurat et al, 2002), cell membrane repair (Cai et al, 2009), synaptic vesicle exocytosis (Li et al, 2001), senescence (Pearson et al, 2000), stem cell pluripotency (Sato et al, 2012) and erythrocyte differentiation (Blaybel et al, 2008;Barde et al, 2013), as well as the control of viral, bacterial and fungal infections (Nisole et al, 2005;Rakebrandt et al, 2014;Cao et al, 2015). These and additional roles are linked to a variety of health conditions.…”

The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway

“…45 Stable overexpression of Flag-TRIM45 proteins was obtained by retroviral infection using pMX-puro expression plasmid followed by puromycin (Sigma) selection as described. 46 For serum induction assay, as stimulation of growthpromoting extracellular stimuli in quantitative reverse transcriptase-PCR assays, western blotting and ChIP, we washed cell cultures at 50-70% confluency twice with phosphate-buffered saline, starved them in serumfree medium for >12 h and then treated them with serum-containing medium for the times indicated in each figure.…”