Tripeptides From Casein Are Signal Molecules to Induce the Expression of the Extracellular Protease MCP-01 Gene in Marine Bacterium Pseudoalteromonas sp. SM9913

Chen, Xiu-Lan; Yang, Jinyu; Zheng, Xiaoyu; Sheng, Qi; Wang, Lei; Zhang, Yuzhong; Qin, Qi‐Long; Zhang, Xiying

doi:10.3389/fmicb.2019.01354

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…Four tripeptides, SPP, RYP, RQF, and FRQ, derived from casein have significant inducing effect on the expression of the gene encoding extracellular protease MCP-01 in P. sp. SM9913 (Chen et al 2019).…”

Section: Serine Proteasesmentioning

confidence: 99%

Proteases from the marine bacteria in the genus Pseudoalteromonas: diversity, characteristics, ecological roles, and application potentials

Chen

Wang

et al. 2020

Mar Life Sci Technol

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Bacterial strains of the genus Pseudoalteromonas, which includes 48 species, are widely distributed in various ocean environments. Many strains are often abundant and the highest protease producer among marine culturable protease-secreting bacteria, suggesting their important role in marine organic nitrogen degradation and cycling. The extracellular proteases of Pseudoalteromonas are diverse, including serine proteases, metalloproteases, and cystine proteases. Pseudoalteromonas proteases have unique properties, such as cold adaptation, salt tolerance, distinct substrate specificity, and catalytic mechanism. They play important ecological roles in marine organic nitrogen degradation and in the interactions of Pseudoalteromonas with other organisms. Some Pseudoalteromonas proteases have shown promising application potentials in bioactive peptide preparation and meat processing. In this review, advances in our knowledge of Pseudoalteromonas proteases are introduced, with a focus on diversity, characters, ecological significance, and application potentials.

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Section: Serine Proteasesmentioning

confidence: 99%

Proteases from the marine bacteria in the genus Pseudoalteromonas: diversity, characteristics, ecological roles, and application potentials

Chen

Wang

et al. 2020

Mar Life Sci Technol

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“…This discovery confirmed that the protein utilized by L. helveticus could stimulate protein translation. A few studies examined how casein influences gene expression during L. helveticus growth ( Chen et al, 2019 ; Zhao et al, 2019 ). However, most of them concluded with analyses of the expression levels of the enzymes involved in bacterial hydrolysis and did not address the changes occurring in transcription or translation.…”

Section: Discussionmentioning

confidence: 99%

Integrated Transcriptome and Proteome Analyses Reveal Protein Metabolism in Lactobacillus helveticus CICC22171

Wang

et al. 2021

Front. Microbiol.

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Lactobacillus helveticus is a homofermentative lactic acid bacterium. It is widely used in the fabrication of Swiss cheese and other dairy products. The aim of this study was to elucidate the mechanism by which L. helveticus utilizes protein. Lactobacillus helveticus CICC22171 were cultured in two different media with various nitrogen sources. The control contained 20 basic amino acids, while the experimental medium contained casein. De novo transcriptome and isobaric tags for relative and absolute quantification (iTRAQ) proteome analyses were applied to determine how L. helveticus utilizes protein. The casein underwent extracellular hydrolysis via ATP-binding cassette (ABC) transporter upregulation and Mn2+-associated cell envelope proteinase (CEP) downregulation. Sigma factors and EF-Tu were upregulated and Mg2+ was reduced in bacteria to accommodate DNA transcription and protein translation in preparation for proteolysis. Hydrolase activity was upregulated to digest intracellular polypeptides and control endopeptidase genes. In these bacteria, casein utilization affected glycolysis, trehalose phosphotransferase system (PTS), and key factors associated with aerobic respiration and reduced glucose consumption.

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“…The expression of mcp01 and production of MCP-01 in strain SM9913 can be induced in the presence of casein but not Casamino Acids, indicating that peptides rather than amino acids derived from casein induce the expression of the gene mcp01 in SM9913. Then, the above-mentioned effective tripeptides were identified from the hydrolysate of casein treated by the ex-proteases of this strain (30). On the other hand, the ex-protease synthesis of Bacillus was reported to be induced by substantially decreased concentrations of amino acids during the exponential growth phase (31,32).…”

Section: Inducers For Microbial Ex-protease Productionmentioning

confidence: 96%

Mechanisms for Induction of Microbial Extracellular Proteases in Response to Exterior Proteins

Zhang

Chen

2020

Appl Environ Microbiol

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Proteins are a main organic nitrogen source for microorganisms. Many heterotrophic microorganisms secrete extracellular proteases (ex-proteases) to efficiently decompose proteins into oligo-peptides and amino acids when exterior proteins are required for growth. These ex-proteases not only play important roles in microbial nutrient acquisition or host infection but also contribute greatly to the global recycling of carbon and nitrogen. Moreover, a lot of microbial ex-proteases have important applications in industrial, medical and biotechnological areas. Therefore, uncovering the mechanisms by which microorganisms initiate the expression of ex-protease genes in response to exterior proteins is of great significance. In this review, the progress made in understanding the induction mechanisms of microbial ex-proteases in response to exterior proteins is summarized, with a focus on the inducer molecules, membrane sensors and downstream pathways. Problems to be solved for better understanding of the induction mechanisms of microbial ex-proteases are also discussed.

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Tripeptides From Casein Are Signal Molecules to Induce the Expression of the Extracellular Protease MCP-01 Gene in Marine Bacterium Pseudoalteromonas sp. SM9913

Cited by 4 publications

References 29 publications

Proteases from the marine bacteria in the genus Pseudoalteromonas: diversity, characteristics, ecological roles, and application potentials

Proteases from the marine bacteria in the genus Pseudoalteromonas: diversity, characteristics, ecological roles, and application potentials

Integrated Transcriptome and Proteome Analyses Reveal Protein Metabolism in Lactobacillus helveticus CICC22171

Mechanisms for Induction of Microbial Extracellular Proteases in Response to Exterior Proteins

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