2020
DOI: 10.3390/ijms21228469
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Triplex Real-Time PCR Approach for the Detection of Crucial Fungal Berry Pathogens—Botrytis spp., Colletotrichum spp. and Verticillium spp.

Abstract: Phytopathogens cause undeniably serious damage in agriculture by harming fruit cultivations and lowering harvest yields, which as a consequence substantially reduces food production efficiency. Fungi of the Botrytis, Colletotrichum and Verticillium genera are a main concern in berry production. However, no rapid detection method for detecting all of these pathogens simultaneously has been developed to date. Therefore, in this study, a multiplex real-time PCR assay for this purpose was established. Universal fu… Show more

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Cited by 19 publications
(12 citation statements)
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“…were isolated from infected strawberry roots ( Oszust et al, 2020 ), while Colletotrichum sp. was isolated from infected strawberry fruit ( Malarczyk et al, 2020 ) at the Institute of Agrophysics of the Polish Academy of Sciences (Lublin, Poland). In the case of B. cinerea and Colletotrichum sp.…”
Section: Methodsmentioning
confidence: 99%
“…were isolated from infected strawberry roots ( Oszust et al, 2020 ), while Colletotrichum sp. was isolated from infected strawberry fruit ( Malarczyk et al, 2020 ) at the Institute of Agrophysics of the Polish Academy of Sciences (Lublin, Poland). In the case of B. cinerea and Colletotrichum sp.…”
Section: Methodsmentioning
confidence: 99%
“…and Verticillium spp. Phytopathogens characteristics including a way and source of isolation, as well as identification within GenBank accession numbers list were described previously by Malarczyk et al [ 66 ]. The first step was to suspend centrifuged bacteria in 1 mL of water.…”
Section: Methodsmentioning
confidence: 99%
“…G408/18, G368/18, G369/18, and G373/18. More descriptive information concerning the selected strains was presented by Malarczyk et al [83].…”
Section: Isolation and Identificationmentioning
confidence: 99%