Tripropeptin C Blocks the Lipid Cycle of Cell Wall Biosynthesis by Complex Formation with Undecaprenyl Pyrophosphate

Daptomycin-Mediated Reorganization of Membrane Architecture Causes Mislocalization of Essential Cell Division Proteins

“…While this is an attractive hypothesis, there is little direct evidence to support such a model, as daptomycin does not inhibit any of the enzymatic steps in cell wall biosynthesis that have been tested so far (19,46,49). Studies of daptomycin-resistant mutants isolated in a number of different species also generally support the idea that the membrane is the major target for daptomycin antibacterial activity.…”

Lipodepsipeptide Empedopeptin Inhibits Cell Wall Biosynthesis through Ca2+-dependent Complex Formation with Peptidoglycan Precursors

“…While work on this publication was in progress, it was reported that tripropeptin C can bind to undecaprenyl-pyrophosphate (C 55 -PP), thereby inhibiting the activity of undecaprenyl-pyrophosphate phosphatase (UppP) (9). Here, we show that empedopeptin exerts its antibacterial action by inhibition of peptidoglycan synthesis.…”

Inhibition of the First Step in Synthesis of the Mycobacterial Cell Wall Core, Catalyzed by the GlcNAc-1-phosphate Transferase WecA, by the Novel Caprazamycin Derivative CPZEN-45

“…avium ATCC25291 incubation for 2-21 days at 37°C and against other bacteria after incubation for 18 h at 37°C. Inhibition of Macromolecular Synthesis-Inhibition of macromolecular synthesis was assayed by measurement of the incorporation of radiolabeled precursors into peptidoglycan, teichoic acid, fatty acid, DNA, RNA, and protein in the precipitate of a 10% trichloroacetic acid (TCA) extract of the cell as reported previously (14,15). Log phase cell cultures (optical density at 600 nm (OD 600 ) ϭ 0.2) of B. subtilis 168 grown in nutrient broth were transferred to 96-well plates at 90 l per well and then preincubated at 37°C for 5 min with antibiotics.…”

“…Evaluation of Enzyme Activity of MraY and TagO of B. subtilis-Enzyme activity of MraY and TagO was measured as described previously with some modifications (14,17). The cell lysates of B. subtilis 168-Yex and 168-Oex were used for the enzyme sources of MraY and TagO, respectively.…”

“…Nutrient broth consisted of 1% polypeptone (Nihon Pharmaceutical Co., Ltd., Tokyo, Japan), 1% nutrient (Kyokuto, Tokyo, Japan), and 0.2% NaCl in deionized water (pH was adjusted to 7.0 before sterilization). After preincubation, 10 l of 10 nCi/l N-acetyl-D-[1- 14 H]leucine were added to measure peptidoglycan, teichoic acid, fatty acid, DNA, RNA, and protein synthesis, respectively. All radiolabeled compounds were purchased from PerkinElmer Life Sciences.…”