Triptolide induces cell-cycle arrest and apoptosis of human multiple myeloma cells in vitro via altering expression of histone demethylase LSD1 and JMJD2B

Abstract

Aim: To elucidate the relationship between triptolide-induced changes in histone methylation and its antitumor effect on human multiple myeloma (MM) cells in vitro. Methods: Human multiple myeloma cell line RPMI8226 was used. Apoptosis was evaluated using Annexin-V-FITC/PI-labeled flow cytometry, Hoechst 33258 staining, and transmission electron microscopy. Flow cytometry was used to detect the cell cycle distribution of the apoptotic cells. The presence of the LSD1, JMJD2B, H3K4me2, H3K9me2, and H3K36me2 proteins was verified by Western blot analysis. Semi-quantitative real-time PCR was performed to examine the expression of LSD1 and JMJD2B.

Protection of Quercetin against Triptolide-induced apoptosis by suppressing oxidative stress in rat Leydig cells

“…(TWHF), is a diterpene triepoxide with variety biological activities, such as anti-inflammatory, anti-cancerogenic, immunomodulatory and pro-apoptotic activities [1,2]. However, its potential toxicity to circulatory and reproductive system limits its clinical application [3].…”

Combined Effects of Curcumin and Triptolide on an Ovarian Cancer Cell Line

“…Recently, curcumin (Yu et al, 2011;Li et al, 2013;Zhou et al, 2013;Yin et al, 2013) and triptolide (Kim et al, 2010;Liu et al, 2012;Wen et al, 2012) are able to potently inhibit the growth of human cancer cells in vitro and prevents tumor growth in vivo via inhibiting cell proliferation and inducing apoptosis. However, both of curcumin and triptolide possess side effects in high concentration (Banjerdpongchai et al, 2005;Clawson et al, 2010;Antonoff et al, 2010;Li et al, 2010;Borja-Cacho et al, 2010;Shakibaei et al, 2013;Du et al, 2013;Jiang et al, 2013).…”

Raman spectrum reveals the cell cycle arrest of Triptolide-induced leukemic T-lymphocytes apoptosis

“…While, some research results found that TPL possibly played an important role in blocking protein synthesis and inhibiting cell proliferation by inhibiting the activity of RNA polymerase and affecting its transcription [5,10]. Specially, it was reported that TPL-induced cell apoptosis was possibly performed by cell cycle G1 phase arrest [11], but its mechanism is still unclear and remains to be elucidated [12]. To date, there is no biochemical changes information conceiving whether TPL induces cell cycle G1 phase arrest or not.…”