Triptolide therapy for neuroblastoma decreases cell viability in vitro and inhibits tumor growth in vivo

Antonoff, Mara B.; Chugh, Rohit; Borja–Cacho, Daniel; Dudeja, Vikas; Clawson, Kimberly A.; Skube, Steven J.; Sorenson, Brent S.; Saltzman, Daniel A.; Vickers, Selwyn M.; Saluja, Ashok K.

doi:10.1016/j.surg.2009.04.023

Cited by 82 publications

(76 citation statements)

References 24 publications

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“…Interestingly, compared with PC-3 cells, LNCaP cells showed more sensitivity to Triptolide since higher cell inhibition rate was achieved, which might be due to its intrinsic characteristics. Although several targets involved in the antitumor activity of Triptolide have been described (Kiviharju et al, 2002;Phillips et al, 2007;Antonoff et al, 2009;Liu et al, 2009;Zhu et al, 2009;Wang et al, 2009;Zhao et al, 2010;Huang et al, 2012), its mechanism of action is still not fully elucidated. EZH2 has offered a potential target for Triptolide in anti-PCa treatment.…”

Section: Discussionmentioning

confidence: 99%

“…The inhibitory activity of Triptolide on various cancer cells growth has been studied (Kiviharju et al, 2002;Phillips et al, 2007;Antonoff et al, 2009;Liu et al, 2009;Zhu et al, 2009;Wang et al, 2009;Zhao et al, 2010). Here we determined the effect of Triptolide on the growth of PCa cells.…”

Section: Triptolide Inhibits the Growth Of Pca Cellsmentioning

confidence: 99%

“…The antitumor activity of Triptolide, one of the main active ingredients in the traditional Chinese medicine Tripterygium wilfordii Hook F, is well documented (Kiviharju et al, 2002;Phillips et al, 2007;Antonoff et al, 2009;Liu et al, 2009;Zhu et al, 2009;Wang et al, 2009;Zhao et al, 2010;Huang et al, 2012). Its antitumor activity involves a variety of cell signaling pathways and protein modification processes.…”

Section: Introductionmentioning

confidence: 99%

“…Its antitumor activity involves a variety of cell signaling pathways and protein modification processes. Several targets of Triptolide have been described in a variety of tumors, such as the vascular endothelial growth factor (Zhu et al, 2009), NF-kappa B (Zhu et al, 2009), Hsp70 (Westerheide et al, 2006;Phillips et al, 2007;Antonoff et al, 2009). Triptolide also was proven to inhibit the expression of the SUMO-specific protease 1, the androgen receptor and c-Jun (Huang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Triptolide Inhibits Histone Methyltransferase EZH2 and Modulates the Expression of Its Target Genes in Prostate Cancer Cells

Tamgue¹,

Chai²,

Lin³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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The histone methyltransferase EZH2 (enhancer of zeste homolog 2) plays critical roles in prostate cancer (PCa) development and is a potential target for PCa treatment. Triptolide possesses anti-tumor activity, but it is unknown whether its therapeutic effect relates with EZH2 in PCa. Here we described EZH2 as a target for Triptolide in PCa cells. Our data showed that Triptolide suppressed PCa cell growth and reduced the expression of EZH2. Overexpression of EZH2 attenuated the Triptolide induced cell growth inhibition. Moreover, Triptolide treatment of PC-3 cells resulted in elevated mRNA levels of target genes (ADRB2, CDH1, CDKN2A and DAB2IP) negatively regulated by EZH2 as well as reduced mRNA levelsan of EZH2 positively regulated gene (cyclin D1). Our findings suggest the PCa cell growth inhibition mediated by Triptolide might be associated with downregulation of EZH2 expression and the subsequent modulation of target genes.

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Section: Discussionmentioning

confidence: 99%

Section: Triptolide Inhibits the Growth Of Pca Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Triptolide Inhibits Histone Methyltransferase EZH2 and Modulates the Expression of Its Target Genes in Prostate Cancer Cells

Tamgue¹,

Chai²,

Lin³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Therefore, alternative strategy would be one that not only decreases the dose of chemotherapeutics but enhances the sensitivity of cancer cells to chemotherapeutics. Recently, tumor therapy by traditional Chinese herb such as curcumin (Gandhy et al, 2012;Khaw et al, 2013;Blakemore et al, 2013) and triptolide (Phillips et al, 2007;Matsui et al, 2008;Antonoff et al, 2009;Zhu et al, 2012;Dudeja et al, 2013) is becoming more and more attractive (Du et al, 2013). We therefore attempted to examine the effects of curcumin and triptolide on ovarian cancer.…”

Section: Discussionmentioning

confidence: 99%

Combined Effects of Curcumin and Triptolide on an Ovarian Cancer Cell Line

Cai

Lin²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: As natural medicines in Asia, curcumin and triptolide extracted from different drug plants have proven to possess anticancer potential and widely used for anti-cancer research. The present study attempted to clarify that curcumin and triptolide synergistically suppress ovarian cancer cell growth in vitro. Methods: To test synergic effects, cell viability and apoptosis were analyzed after curcumin and triptolide combination treatment on ovarian cancer cell lines. Synergistic effects on apoptosis induction were determined by lactate dehydrogenase (LDH) leakage assay, intracellular reactive oxygen species (ROS) assay, mitochondrial membrane potential (MMP) loss assay and flow cytometry analysis. Critical regulators of cell proliferation and apoptosis related were analyzed by qRT-PCR and Western blotting. Results: We showed that the combination of curcumin and triptolide could synergistically inhibit ovarian cancer cell growth, and induce apoptosis, which is accompanied by HSP27 and HSP70, indicating that HSP27 and HSP70 play the important role in the synergic effect. Conclusions: From the result present here, curcumin and triptolide combination with lower concentration have a synergistic anti-tumor effect on ovarian cancer and which will have a good potential in clinical applications.Keywords: Curcumin -triptolide -ovarian cancer cell line -cytotoxicity RESEARCH ARTICLE Combined Effects of Curcumin and Triptolide on an Ovarian Cancer Cell LineYing-Ying Cai*, Wei-Ping Lin, Ai-Ping Li, Jian-Yang Xu Pacak et al., 2012;Hsu et al.,2013;Tan et al., 2013;Huang et al., 2013). Recently, curcumin (Yu et al., 2011;Li et al., 2013;Zhou et al., 2013;Yin et al., 2013) and triptolide (Kim et al., 2010;Liu et al., 2012;Wen et al., 2012) are able to potently inhibit the growth of human cancer cells in vitro and prevents tumor growth in vivo via inhibiting cell proliferation and inducing apoptosis. However, both of curcumin and triptolide possess side effects in high concentration (Banjerdpongchai et al., 2005;Clawson et al., 2010;Antonoff et al., 2010;Li et al., 2010;Borja-Cacho et al., 2010;Shakibaei et al., 2013;Du et al., 2013;Jiang et al., 2013).To decrease the side effects and enhance the efficacy of traditional Chinese medicine (TCM) prescriptions, combinations of different TCM herbs is alternative strategy for cancer therapy. The present study was aimed to analysis the combined effect of curcumin and triptolide on ovarian cancer cell lines in vitro. Materials and Methods Reagents and cell cultureCurcumin was purchased from the Sigma-Aldrich Trading Co, Ltd (Shanghai, China); it was dissolved in DMSO to obtain 1 mM stock solution and kept at -20 °C until use, and then diluted in medium to different concentrations. Triptolide (purity > 99.0%, Institute of Human ovarian cancer OVAR3, SKOV3, HO-8910 and A2780 cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10 % fetal bovine serum (F...

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Natural Products Targeting Clinically Relevant Enzymes of Eicosanoid Biosynthesis Implicated in Inflammation and Cancer

Reddy

Yarla

Ediga

et al. 2017

Natural Products Targeting Clinically Relevant Enzymes

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Triptolide therapy for neuroblastoma decreases cell viability in vitro and inhibits tumor growth in vivo

Cited by 82 publications

References 24 publications

Triptolide Inhibits Histone Methyltransferase EZH2 and Modulates the Expression of Its Target Genes in Prostate Cancer Cells

Triptolide Inhibits Histone Methyltransferase EZH2 and Modulates the Expression of Its Target Genes in Prostate Cancer Cells

Combined Effects of Curcumin and Triptolide on an Ovarian Cancer Cell Line

Natural Products Targeting Clinically Relevant Enzymes of Eicosanoid Biosynthesis Implicated in Inflammation and Cancer

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