Triterpenes From Ceriops Decandra (Griff.) W. Theob.

Perez, Judy; Shen, Chien‐Chang; Ragasa, Consolacion Y.

doi:10.22159/ajpcr.2017.v10i11.19461

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…C. decandra is a salt tolerant plant and possesses unique bioactive metabolites. Lupane-triterpene esters and other triterpene constituents have been isolated from the leaves of C. decandra [36,37].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Activity of a Medicinal Mangrove Plant Ceriops Decandra (Griff.) Ding Hou Leaves on Human Breast Adenocarcinoma Cell Line McF-7

Sukasini¹,

M²

2018

Asian J Pharm Clin Res

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Objective: Ceriops decandra (Griff.) Ding Hou (Rhizophoraceae) is an evergreen mangrove tree widely used in folklore medicine for the treatment of several diseases. Concerning the presence of anticancer molecule in C. decandra, the present investigation was carried out to study the preliminary phytochemical screening, in vitro cytotoxic effect, and apoptotic induction of methanol extract of C. decandra.Methods: The preliminary phytochemical investigations were done using standard methods. Cytotoxic activity was analyzed in terms of brine shrimp (Artemia salina Leach) lethality bioassay. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay was used to evaluate the cell viability and growth inhibition of MCF-7 (human breast adenocarcinoma) cells after treatment with different doses of C. decandra extract. Cell morphological changes induced by the extract were observed using phase contrast inverted microscope. Induction of apoptosis was determined by DNA fragmentation assay.Results: Phytochemical screening revealed the presence of various potent phytochemicals. In the brine shrimp lethality bioassay, the LC50 of the extract was found to be 12.5 μg/mL, implying a promising cytotoxic effect. The MTT assay results showed increased inhibitory activity at a concentration-dependent manner and altered the cell morphology after exposure with C. decandra extract which exhibited typical apoptotic morphological changes. Furthermore, the characteristic DNA ladder pattern of C. decandra treated cells in agarose gel electrophoresis confirms the induction of apoptosis.Conclusion: The research revealed the cytotoxic effect of C. decandra on MCF-7 cells through its nature of induction of apoptosis.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic Activity of a Medicinal Mangrove Plant Ceriops Decandra (Griff.) Ding Hou Leaves on Human Breast Adenocarcinoma Cell Line McF-7

Sukasini¹,

M²

2018

Asian J Pharm Clin Res

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“…The most promising approach in therapy is the use of natural substances with cytotoxic activity concerning tumor cells but not affecting healthy ones. Such substances include lupane triterpenoids-betulin, betulinic and betulonic acids and their derivatives [1][2][3][4][5][6][7]. Recent studies have shown that the combination of triterpenoids such as oleanolic, ursolic and betulinic acids with 5-fluorouracil might be a useful tool with reduced doses of an anticancer drug-5-fluorouracil and without any side effects.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, Metabolic and Antitumor Activity of Triterpenoids Combination With Cytostatics

Vorobyova

Grubova

Belyaeva

et al. 2020

Int J Pharm Pharm Sci

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Objective:To study the effect of betulin derivatives combination with 5-fluorouracil or hydrazine sulfate on the ROS generation, the SOD and LDH activity using rat blood, as well as the effect of combination drugs on Ehrlich carcinoma in experiments on mice. Methods:We used a chemiluminescence technique to study the ROS generation, and spectrophotometry to determine the MDA level and the SOD and LDH activity. The model of transplanted Ehrlich ascites carcinoma was investigated on mice using a cytological analysis of ascitic fluid cells according to Pappenheim`s method. Results:In vitro experiments on rat blood at the doses of 2, 5 and 10 μg per ml revealed the dose-dependent effect of combination drugs on the antioxidant properties. In plasma, the ROS generation and the MDA level increased by 10-300% in comparison with control at the doses of 5 and 10 μg per ml only. Still, the SOD and LDH activity in general increased by 10-130% in comparison with control under the action of the studied combination drugs. The study on mice showed the effectiveness of a combination of triterpenoids and cytostatics in Ehrlich ascites carcinoma therapy. The state and behavior of the animals improved, the volume of ascites fluid decreased by 40-50% after treatment for 10 d. Conclusion:The combination of betulin derivatives with cytostatics can be used as antitumor drugs in Ehrlich ascites carcinoma therapy that is due to metabolic plasticity, increased ROS generation in enhanced antioxidant enzyme protection.

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“…Lupane triterpenoids such as betulin (lup-20(29)-ene-3,28-diol) and its derivatives extracted from renewable natural resources [1][2][3], e. g. from birch bark, exhibit various types of pharmacological activity. The antioxidant, anti-inflammatory, hepatoprotective, antimutagenic, and other activities of the compounds were demonstrated by in vitro and in vivo experiments [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

The Improvement of Betulin-3, 28-Diphosphate Water-Solubility by Complexation With Amines–meglumine and Xymedon

Мельникова

Malygina

Panteleev

et al. 2019

Int J Pharm Pharm Sci

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Objective: To study betulin-3,28-diphosphate (BDP) water solubility improved by forming salt complexes with hydrophilic amino alcohols: meglumine as acidosis corrector and xymedon as the water-soluble antioxidant. Methods: We used 13C-, 31P-NMR, UV-spectroscopy and potentiometric titration to study the BDP-amine salt complexes formation and their solubility using HPLC-analysis. Results: The participation of xymedon in the proton transfer reaction with BDP in aqueous solutions was confirmed by the bathochromic shift of the carbonyl band from 299.1 nm to 304.2 nm, and by a hyperchromic effect (molar extinction ε from 8508 to 10 441 l·mol-1·cm-1) in UV-spectra. BDP complexation with meglumine was estimated by UV-spectral molar ratio method at 256 nm. Molar ratio of BDP-amine complexes (1:4) was proved by 31P-NMR. The chemical shift of phosphorus at C-3 atom of BDP (δ =-0.58 ppm) changed to+3.39 ppm, and at C-28 atom (δ =+0.28 ppm)–to+4.60 ppm. BDP solubility increased 100-600 fold according to HPLC-analysis. Conclusion: BDP interaction with amine in an aqueous solution was shown to proceed via a proton transfer due to relatively weak forces such as London forces, hydrogen bonding, electrostatic and hydrophobic interactions. In general, the formation of BDP salt complexes with amines in solution determines BDP water solubility. Water-soluble BDP enables to develop hydrophilic dosage forms.

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Triterpenes From Ceriops Decandra (Griff.) W. Theob.

Cited by 6 publications

References 18 publications

Cytotoxic Activity of a Medicinal Mangrove Plant Ceriops Decandra (Griff.) Ding Hou Leaves on Human Breast Adenocarcinoma Cell Line McF-7

Cytotoxic Activity of a Medicinal Mangrove Plant Ceriops Decandra (Griff.) Ding Hou Leaves on Human Breast Adenocarcinoma Cell Line McF-7

Antioxidant, Metabolic and Antitumor Activity of Triterpenoids Combination With Cytostatics

The Improvement of Betulin-3, 28-Diphosphate Water-Solubility by Complexation With Amines–meglumine and Xymedon

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