Triterpenoids and polysaccharide peptides-enriched Ganoderma lucidum : a randomized, double-blind placebo-controlled crossover study of its antioxidation and hepatoprotective efficacy in healthy volunteers

Abstract

Context:Ganoderma lucidum (Leyss: Fr) Karst. (Polyporaceae) is an oriental medicinal fungus, commonly used in traditional Chinese medicine (TCM) for treating various condition or diseases such as hypertension, hyperglycaemia, hepatitis and cancer.Objective: The current study examines whether triterpenoids and polysaccharide-enriched G. lucidum (GL) influence antioxidation and hepatoprotective efficacy by suppressing oxidative stress.Materials and methods: Forty-two healthy subjects (22 male and 20 female) were recruited and segregated into two groups as experimental or placebo and requested to intake GL (n = 21) or placebo (n = 21) capsule (225 mg; after lunch or dinner) for six consecutive months and vice versa with one month washout period in between. The anthropometric analysis and biochemical assays, as well as abdominal ultrasonic examination were performed.Results: Consumption of GL substantially improved (p < 0.05) the total antioxidant capacity (TEAC; 79.33–84.04), total thiols and glutathione content (6–8.05) in plasma as well as significant (p < 0.05) enhanced the activities of antioxidant enzymes. Whereas, the levels of thiobarbituric acid reactive substances (TBARS; 3.37–2.47), 8-hydroxy-deoxy-guanosine (8-OH-dG; 15.99–11.98) and hepatic marker enzymes (glutamic-oxaloacetic transaminase; GOT and glutamic-pyruvic transaminase; GPT) were concomitantly reduced (42 and 27%) on treatment with GL. Furthermore, the abdominal ultrasonic examination in GL subjects displayed a notable alteration on hepatic condition by reversing from mild fatty liver condition (initial) to normal condition.Discussion and Conclusion: The outcome of the present intervention demonstrated the antioxidation, anti-aging and hepatoprotective nature of GL by effectively curbing oxidative stress.

Connection of Ganoderma Lucidum with Health

“…Furthermore, the hepatic steatosis state rate decreased through examining the hepatic enzyme activities and conducting an ultrasound. 84 Another study analyzed content of eight mushroom species and found G. lucidum to have the highest total phenolic content (TPC) (39.3±0.07 mg GAE/g), total flavonoid content (TFC) (15.1±0.04 mg GAE /g) and the strongest free radical scavenging activity (IC50=3.66 mg/mL). TPC of the other mushroom examined in the study ranged from 3.9±0.04 (Tremella fuciformis) to 11.8±0.06 (Agrocybeaegerita) mg GAE/g and TFC ranged from 0.75±0.04 (Grifolafrondosa) to 4.29±0.04 (Auricularia auricula-judae) mg GAE/g.…”

Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with HPLC and Artificial Neural Network Analysis for Ganoderma lucidum

“…It is one of the most highly used medicinal fungi around the world [4]. Studies have shown that G. lucidum has the pharmacological effect of immunoregulation [5], antitumor [6,7], liver protection [8], and blood glucose reduction [9]. Ganoderic acid A and D ( Figure 1) are the main triterpenoids in G. lucidum [4].…”

Ganoderma lucidum polysaccharide inhibits prostate cancer cell migration via the protein arginine methyltransferase 6 signaling pathway

“…It has been reported that Ganoderma lucidum ( G. lucidum ) exhibited preventive and therapeutic effects in cancer ( 15 ), chronic bronchitis ( 16 ), bronchial asthma ( 17 ) and hepatitis ( 18 ). Polysaccharides, which consist of glucose, mannose, galactose, xylose, fucose and arabinose, are one of the most important active components of G. lucidum ( 19 ).…”