Tritium uptake in rainbow trout (Oncorhynchus mykiss): HTO and OBT-spiked feed exposures simultaneously

Kim, Sang Bog; Shultz, Carmen; Stuart, Marilyne; Festarini, Amy

doi:10.1016/j.apradiso.2015.01.020

Cited by 16 publications

(10 citation statements)

References 17 publications

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“…The dilution factor was taken into account when the activity concentrations were calculated. The protocol for OBT measurement in animals is described in Kim et al (2015) and Gagnaire et al (2017). Samples of nontritiated Silver Cup feed were also prepared as described in Gagnaire et al (2017).…”

Section: Tfwt and Obt Measurements And Estimated Dose Rate In Fish Samentioning

confidence: 99%

“…This was not the case in the present study. As for the field study (Gagnaire et al 2017), this was expected as it takes time for OBT to accumulate in fish tissues and it also takes a long time for it to depurate (Kim et al 2015). However, we used our data to evaluate HTO transfer and transformation of internalized TFWT in OBT because this is currently poorly documented (Melintescu &Galeriu 2011).…”

Section: Tfwt and Obt In Fish Tissuesmentioning

confidence: 99%

“…Therefore, this study was followed up with a controlled laboratory study in which fathead minnows chronically exposed to low dose tritium spanning a similar range as that found at the field sites were tested to assess genotoxic, immune and neurological responses. Two forms of tritium (HTO and OBT) were assessed because tritium toxicity may also vary as a function of its chemical form (Kim et al 2015). Minnows were housed in aquaria and exposed over 60 days to water with a range of HTO activity concentrations.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of in vivo exposure to tritium: a multi-biomarker approach using the fathead minnow, Pimephales promelas

Gagnaire

Gosselin

Festarini

et al. 2018

Environ Sci Pollut Res

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Highlights-Following a previously published field investigation, this study aims to characterize tritium effects on fish health under controlled conditions in a laboratory setting.-Fathead minnows were exposed to tritium activity concentrations up to 180,000 Bq/L.-At the highest levels of exposure, tritium increased DNA damage and modulated the immune responses.-Other markers were affected, including the neural system, oxidative stress and fatty acid composition.-No effects are reported on the measured health indices and anti-oxidant activities.

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Section: Tfwt and Obt Measurements And Estimated Dose Rate In Fish Samentioning

confidence: 99%

Section: Tfwt and Obt In Fish Tissuesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of in vivo exposure to tritium: a multi-biomarker approach using the fathead minnow, Pimephales promelas

Gagnaire

Gosselin

Festarini

et al. 2018

Environ Sci Pollut Res

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“…Exchanges of HTO between water and organisms are rapid: maximal dose rates were obtained for 24-h eggs. Several studies showed that tritium accumulated in adult organisms 7: Relative expression of genes (normalized to reference gene ef1) compared to control in larvae contaminated during 10 d at 10 4 , 10 5 , and 10 6 Bq/mL of tritiated water (n = 9) a 4 dpf 7 dpf 10 dpf 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL ache equilibrates with HTO concentration of the water and that the fraction of tritium bound to organic matter equilibrates with the concentration of HTO internalized (Galeriu and Melintescu 2011;Kim and Korolevych 2013;Melintescu et al 2015;Kim et al 2015Kim et al , 2019Gagnaire et al 2017Gagnaire et al , 2020. A companion paper study presented in detail the discussion about internalization results obtained after tritium contamination for 2 stages (24-h eggs and 4-dpf larvae) and 2 HTO concentrations (10 5 and 10 6 Bq/mL; Arcanjo et al 2019).…”

Section: Dose Rates Calculated For Htomentioning

confidence: 99%

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early-Life Stages

Gagnaire

Arcanjo

Cavalié

et al. 2019

Environmental Toxicology and Chemistry

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Tritium, a radioactive isotope of hydrogen of natural and anthropogenic origin, is ubiquitously present in the environment. Effluents of nuclear centers of production are significant anthropogenic sources. With the upcoming project of thermonuclear fusion, tritium releases in the environment may increase. It is therefore important to characterize the ecological risk linked to tritium. The effects of tritiated water (HTO) were therefore studied in zebrafish larvae exposed for 10 d to different dose rates, 1.1 × 102, 4.1 × 102, and 3.8 × 103 µGy/h for larvae corresponding, respectively, to a water contamination of 104, 105, and 106 Bq/mL of HTO. Those dose rates were higher than 10 μGy/h, which is the threshold recommended to start monitoring ecosystems where radiological contaminants are present. Mortality, embryo‐larval development, immune toxicity, genotoxicity, neurotoxicity, and alterations of tissues were investigated. The results showed that HTO exposure induced DNA damage and reactive oxygen species production and modulated the expression of genes involved in detoxification processes. Moreover, modifications of the muscular tissues (degradation of myofibrils at 4 d post fertilization and disorganization of mitochondria at later stages) were observed. The results differed with HTO dose rates and with developmental stages. These results will drive future research for the development of new HTO‐sensitive biomarkers and will allow us to progress in the characterization of the modes of action of tritium in fish. Environ Toxicol Chem 2020;39:648–658. © 2019 SETAC

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“… 1 - 4 The half-life of tritium is 12.33 years, and the average penetration distance of its electrons in water is 0.56 μm, which is considerably less than the average diameter of a human cell. 5 , 6 Therefore, it does not cause damage due to external irradiation but rather long-term internal radiation that results from the inhalation, oral intake, or absorbance of radioactive particles through the skin. After entering an organism, tritium can replace hydrogen atoms in biological macromolecules or bind to cellular components to form organically bound tritium (OBT).…”

Section: Introductionmentioning

confidence: 99%

Tritiated Water Induces Toxicity in Human Umbilical Vein Vascular Endothelial Cells via IL8

Yan

Liu

et al. 2020

Dose-Response

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We aimed to determine the toxic effects of tritiated water (HTO) on 12 generations (T1-T12) of human umbilical vein vascular endothelial cells (HUVECs) and elucidate the underlying mechanisms. We evaluated cellular senescence, interleukin (IL) 8 concentrations, and angiogenesis using β-galactosidase staining, enzyme-linked immunosorbent assay, and in vitro assays, respectively. The adhesion properties of contaminated cells and differentially expressed genes were assessed using the xCELLigence RTCA SP system and gene chip analysis, respectively. We found that long-term exposure to low levels of HTO can reduce the adhesion of HUVECs to the cellular matrix as well as their angiogenic capacity, while increasing their permeability, senescence, and adhesion to monocytes. Interleukin 8 activated the p38 and Epidermal Growth Factor Receptor (EGFR) pathways in HTO-treated cells and hence was identified as a key candidate of biomarker. The present study clarified the toxicity of HTO in vascular endothelial cells and identified IL8 as a novel protective target with important theoretical and practical values.

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Tritium uptake in rainbow trout (Oncorhynchus mykiss): HTO and OBT-spiked feed exposures simultaneously

Cited by 16 publications

References 17 publications

Effects of in vivo exposure to tritium: a multi-biomarker approach using the fathead minnow, Pimephales promelas

Effects of in vivo exposure to tritium: a multi-biomarker approach using the fathead minnow, Pimephales promelas

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early-Life Stages

Tritiated Water Induces Toxicity in Human Umbilical Vein Vascular Endothelial Cells via IL8

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