TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi

Pal, Utpal; Li, Xin; Wang, Tian; Montgomery, Ruth R.; Ramamoorthi, Nandhini; deSilva, Aravinda M.; Bao, Fukai; Yang, Xiaofeng; Pypaert, Marc; Pradhan, Deepti; Kantor, Fred S.; Telford, Sam R.; Anderson, John F.; Fikrig, Erol

doi:10.1016/j.cell.2004.10.027

Cited by 364 publications

(349 citation statements)

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“…Percentage of OspA-and OspC-positive borreliae in midgut and salivary glands of I. ricinus nymphs at different time intervals after capillary feeding. For total numbers refer to Table 1. periods without tick feeding (Fingerle et al, 1995;Pal et al, 2004a;Schwan et al, 1995). During the next blood meal, these borreliae restart replication and upregulate OspC, which is associated with migration to the tick salivary glands and infection of the host (Fingerle et al, 1998;Gilmore and Piesman, 2000;Schwan et al, 1995;Schwan and Piesman, 2000).…”

Section: Discussionmentioning

confidence: 99%

Complementation of a Borrelia afzelii OspC mutant highlights the crucial role of OspC for dissemination of Borrelia afzelii in Ixodes ricinus

Fingerle

Goettner

Gern

et al. 2007

International Journal of Medical Microbiology

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Alteration of the outer surface protein (Osp) composition -especially that of OspA and OspC -seems to be important for the adaptation of Borrelia burgdorferi sensu lato to its endothermic hosts (mammals) and poikilothermic vectors (ticks). OspA possibly mediates adherence to tick midgut cells thus enabling the borreliae to survive in the vector, while OspC is associated with borrelial invasion of the tick salivary glands and infection of the mammalian hosts. Here we describe the first successful transformation and complementation of a Borrelia afzelii ospC mutant with the wild-type ospC in trans. To test the influence of OspC on the dissemination behavior in ticks, unfed Ixodes ricinus nymphs were artificially infected by capillary feeding either with B. afzelii wild type, the B. afzelii ospC mutant or the ospC-complemented clone. Tick midguts and salivary glands were investigated after different time intervals for the presence of borreliae and for OspA and OspC by immunfluorescence staining with monoclonal antibodies. While the B. afzelii wild-type strain exhibiting abundant OspC on its surface disseminated to the salivary glands, the OspCnegative mutant was only present in the tick midguts. The ospC-complemented clone which constitutively expresses the wild-type ospC was again able to colonize the salivary glands. This finding demonstrates that OspC is crucial for dissemination of B. afzelii from the tick midgut to the salivary glands, a prerequisite for infection of the warm-blooded host. A summary of the detailed data presented here has already been given in Goettner et al.

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Section: Discussionmentioning

confidence: 99%

Complementation of a Borrelia afzelii OspC mutant highlights the crucial role of OspC for dissemination of Borrelia afzelii in Ixodes ricinus

Fingerle

Goettner

Gern

et al. 2007

International Journal of Medical Microbiology

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“…Concerning the transmission of B. burgdorferi, the OspA protein seems to be the key agent responsible for the growth of spirochetes in ticks. The gene TROSPA coding tick receptor for OspA is strongly expressed in larvae and nymphs, compared to adult ticks [65]. Antunes et al [66] confirmed that receptor TROSPA was over-expressed in Rhipicephalus tick after Babesia bigemina infection, and may be involved in Babesia-tick interactions.…”

Section: Toxoplasma Gondiimentioning

confidence: 99%

Prevalence of infections and co-infections with 6 pathogens in <i>Dermacentor reticulatus</i> ticks collected in eastern Poland

Zając

Wójcik-Fatla

Sawczyn

et al. 2017

Ann Agric Environ Med.

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Occurrence of co-infections with various pathogens in ixodid ticks creates a risk of increased severity of tick-borne diseases in humans and animals exposed to bite of the ticks carrying multiple pathogens. Accordingly, co-infections in ticks were subject of numerous analyses, but almost exclusively with regard to Ixodes ricinus complex whereas potential tick vectors belonging to other genera were much less studied. Taking into consideration the role of Dermacentor reticulatus in the transmission of various pathogens, we carried out for the first time the comprehensive statistical analysis of co-infections occurring in this tick species. An attempt was made to determine the significance of the associations between 6 different pathogens occurring in D. reticulatus (Tick-borne encephalitis virus = TBEV, Anaplasma phagocytophilum, Rickettsia raoultii, Borrelia burgdorferi s. l., Babesia spp., Toxoplasma gondii), using 2 statistical methods: determination of Odds Ratios (ORs) and the Fisher's exact test. 634 questing Dermacentor reticulatus ticks (370 females and 264 males) were collected in 2011-2013 by flagging the lower vegetation in 3 localities in the area of Łęczyńsko-Włodawskie Lakeland, situated in the Lublin region of eastern Poland. The presence of individual pathogens was detected by PCR. Ticks were infected most often with Rickettsia raoultii (43.8%), less with TBEV (8.5%), and much less with Babesia spp., Toxoplasma gondii, Borrelia burgdorferi s.l., and Anaplasma phagocytophilum (2.5%, 2.1%, 1.6% and 1.1%, respectively). The locality-dependent variability proved to be significant for TBEV (c 2 =11.063; P=0.004) and Toxoplasma gondii (c 2 =11.298; P=0.0035), but not for other pathogens. Two hundred seventy (42.6%) of the examined ticks were infected only with a single pathogen, and 54 (8.5%) showed the presence of dual co-infections, each with 2 pathogens. The most common were dual infections with participation of Rickettsia raoultii (7.41%); next, those with participation of the TBEV (5.21%), Toxoplasma gondii (1.58%), Borrelia burgdorferi s.l. (1.26%), Anaplasma phagocytophilum (0.95%), and Babesia spp. (0.63%). On the total number of 15 possible associations, in 9 cases co-infections occurred whereas in 6 cases they were not detected. The most noteworthy were positive co-infections with the participation of TBEV, which proved to be weakly significant (0.05

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“…The attachment of an outer-coat protein (OspA) displayed on the lumenal side of the gut of ticks (like black-legged ticks Ixodes scapularis or Ixodes ricinus) to a receptor (TROSPA) favours B. burgdorferi to persist in the gut and avoid elimination (Pal et al, 2004). The infection may be acquired through larvae or nymphs feeding on small to medium sized wild animals harbouring the B. burgdorferi as reservoir.…”

Section: Pathophysiologymentioning

confidence: 99%

Lyme borreliosis in the horse: A mini-review

Kolk¹

2016

JEBAS

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Lyme borreliosis is a multisystemic tick borne disease (also called Lyme disease in humans) which is caused by the Borrelia burgdorferi sensu lato species complex. It is a thin, elongated Gram-negative bacterium and exhibiting motility with flagellar projections. Affected animals mainly show cranial or peripheral neuropathies and uveitis as the more commonly seen extraneural manifestation. Serological evidences confirm its higher occurrence in elderly horses than young ones. Although, incidence of equine Lyme borreliosis is low, its diagnosis is a real challenge. As no indisputable test exists for detecting antibodies to B. burgdorferi, histopathology remains the gold standard and might reveal vascular sclerosis and pleocellular inflammatory infiltrates in neural tissue. Despite antibiotic treatment clinical signs might progress or recur. This review converses with the disease etiology, pathobiology in brief and its mangment from publich health point of view.

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TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi

Cited by 364 publications

References 20 publications

Complementation of a Borrelia afzelii OspC mutant highlights the crucial role of OspC for dissemination of Borrelia afzelii in Ixodes ricinus

Complementation of a Borrelia afzelii OspC mutant highlights the crucial role of OspC for dissemination of Borrelia afzelii in Ixodes ricinus

Prevalence of infections and co-infections with 6 pathogens in <i>Dermacentor reticulatus</i> ticks collected in eastern Poland

Lyme borreliosis in the horse: A mini-review

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