TRP‐1 expression correlates with eumelanogenesis in human pigment cells in culture

Marmol, Véronique Del; Ito, Shosuke; Jackson, Ian J.; Vachtenheim, J; Berr, Pascale M.; Ghanem, G.; Morandini, Renato; Wakamatsu, Kazumasa; Huez, Georges

doi:10.1016/0014-5793(93)81010-w

Cited by 76 publications

(57 citation statements)

References 19 publications

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“…Moreover, in human cell lines, expression of TRP-1 was only detectable in cells containing eumelanin [43]. The DHI-CA oxidase activity described for TRP-1 implicates this enzyme in the synthesis of eumelanin.…”

Section: Trps and Melanogenesismentioning

confidence: 93%

Tyrosinase and related proteins in mammalian pigmentation

Marmol

Beermann²

1996

FEBS Letters

410

260

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Tyrosinase is the key enzyme in pigment synthesis, initiating a cascade of reactions which convert the amino acid tyrosine to the melanin biopolymer. Two other tyrosinase-related proteins (TRP) are known, TRP-1 (probably DHICAoxidase) and TRP-2 (DOPAchrome tautomerase). These proteins show about 40% homology, and recent results have indicated that the genes might be derived from a common ancestor. We will discuss recent findings on genomic organization, and on the proteins and their presumed function, which is important for eumelanin synthesis in mouse and man.

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Section: Trps and Melanogenesismentioning

confidence: 93%

Tyrosinase and related proteins in mammalian pigmentation

Marmol

Beermann²

1996

FEBS Letters

410

260

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“…In addition to tyrosinase, several other structurally related proteins are encoded by the tyrosinase gene family, notably tyrosinase-related protein 1 (TRP-1) (19,20) and tyrosinase-related protein 2 (TRP-2) (21-23). These two molecules are also involved in the melanin synthesis pathway, both of them contributing more to the synthesis of eumelanin than to pheomelanin (24)(25)(26). TRP-1 and TRP-2 share moderate sequence similarities with tyrosinase, the amino acid homology with the tyrosinase being 43% and 40%, respectively (22,25).…”

Section: Discussionmentioning

confidence: 99%

Immunophenotyping of melanomas for tyrosinase: implications for vaccine development.

Chen

Stockert

Tsang

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

160

104

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Tyrosinase (EC 1.14.18.1), the key enzyme in melanin synthesis, has been shown to be one of the targets for cytotoxic T-cell recognition in melanoma patients. To develop serological reagents useful for immunophenotyping melanoma for tyrosinase, human tyrosinase cDNA was expressed in an Escherichia coli expression vector. The purified recombinant tyrosinase was used to generate mouse monoclonal and rabbit polyclonal antibodies. The prototype monoclonal antibody, T311, recognized a cluster of protein moieties ranging from 70 to 80 kDa in tyrosinase mRNA-positive melanoma cell lines and melanoma specimens as well as in L cells transfected with tyrosinase cDNA. Untransfected L cells and L cells transfected with tyrosinase-related protein 1, TRP-1(gp75), were nonreactive. Immunohistochemical analysis of melanomas with T311 showed tyrosinase in melanotic and amelanotic variants, and tyrosinase expression correlated with the presence of tyrosinase mRNA. Melanocytes in skin stained with T311, whereas other normal tissues tested were negative. The expression pattern of three melanosome-associated proteins-tyrosinase, TRP-l(gp75), and gplOO-in melanoma was also compared. Tyrosinase and gplOO are expressed in a higher percentage of melanomas than TRP-1(gp75), and the expression of these three antigens was discordant. Tyrosinase expression within individual tumor specimen is usually homogenous, distinctly different from the commonly observed heterogenous pattern of gplOO expression.Cutaneous melanin pigments are synthesized by melanocytes.

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“…TRP-1 and TRP-2 reside within the melanosomes and, like tyrosinase, span the melanosomal membrane [15]. It has been suggested that TRP-1 increases the ratio of eumelanin to pheomelanin [16,17]. In addition, they have been demonstrated to increase tyrosinase stability [18,19].…”

Section: Melanogenesismentioning

confidence: 99%

The melanogenesis and mechanisms of skin‐lightening agents – existing and new approaches

Gillbro¹,

Olsson²

2011

Intern J of Cosmetic Sci

315

251

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SynopsisSkin-lightening products are commercially available for cosmetic purposes to obtain lighter skin complexion. Clinically, they are also used for treatment of hyperpigmentary disorders such as melasma, café au lait spot and solar lentigo. All of these target naturally melanin production, and many of the commonly used agents are known as competitive inhibitors of tyrosinase, one of the key enzymes in melanogenesis. In this review, we present an overview of commonly used skin-whitening ingredients that are commercialized, but we also hypothesize on other mechanisms that could be important targets to control skin pigmentation such as for example regulation of the adrenergic and glutaminergic signalling and also control of tetrahydrobiopterins in the human skin. Ré suméLes produits éclaircissants sont disponibles dans le commerce pour des buts cosmétiques afin d'obtenir un tient plus clair. Ils sont égale-ment utilisés en clinique, pour le traitement de troubles hyper pigmentaires comme le melasma, les taches café au lait et le lentigo solaire. Tous ces produits ont pour cible la production naturelle de mélanine et beaucoup de ceux généralement utilisés sont reconnus comme des inhibiteurs compétitifs de la tyrosinase, une des enzymes clés de la mélanogénèse. Dans cette revue, nous présentons une vue d'ensemble des ingrédients généralement utilisés et commercialisés comme blanchissant cutanés mais nous formulons aussi l'hypothèse que d'autres mécanismes pourraient être des cibles importantes pour contrôler la pigmentation de la peau comme par exemple la régula-tion du signal adrénergique et glutaminergique ou le contrôle des tetrahydrobiopterines dans la peau humaine.

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TRP‐1 expression correlates with eumelanogenesis in human pigment cells in culture

Cited by 76 publications

References 19 publications

Tyrosinase and related proteins in mammalian pigmentation

Tyrosinase and related proteins in mammalian pigmentation

Immunophenotyping of melanomas for tyrosinase: implications for vaccine development.

The melanogenesis and mechanisms of skin‐lightening agents – existing and new approaches

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