TRPA1 Contributes to Cold, Mechanical, and Chemical Nociception but Is Not Essential for Hair-Cell Transduction

Kwan, Kelvin Y.; Allchorne, Andrew; Vollrath, Melissa A.; Christensen, Adam; Zhang, Duan-Sun; Woolf, Clifford J.; Corey, David P.

doi:10.1016/j.neuron.2006.03.042

Cited by 1,144 publications

(1,290 citation statements)

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“…Bautista et al [41] found that the percentage of cold-sensitive sensory neurons in culture, number of nocifensive responses to acetone-evoked cooling, paw-withdrawal latencies, and induction of shivering in a cold-plate paradigm (20 to −10°C) were indistinguishable in wild type and TRPA1 −/− mice. In the contrary, Kwan et al [85] did report a decrease number of paw lifts when TRPA1 −/− mice were placed upon a surface cooled to 0°C…”

Section: Mouse Models Deficient In Temperature Sensingmentioning

confidence: 90%

“…Working independently, the laboratories of David Julius and David Corey generated TRPA1-null mice and tested their sensitivity to cold [41,85]. In a remarkable similarity to the discrepancies found with TRPA1 responses to cold in vitro, one group observed no cold sensation deficits in the knockout mice, whereas the second group did.…”

Section: Mouse Models Deficient In Temperature Sensingmentioning

confidence: 91%

“…Similarly, TRPM8-deficient mice have also not been produced; thus, the putative in vivo roles for these two channels have not been addressed experimentally. In contrast of the other TRP channels implicated in mammalian thermosensation, knockout mice for TRPV1, TRPV3, TRPV4, and TRPA1 have been generated and characterized [41,[83][84][85].…”

Section: Mouse Models Deficient In Temperature Sensingmentioning

confidence: 99%

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Temperature sensing across species

McKemy

2007

Pflugers Arch - Eur J Physiol

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The ability to detect changes in temperature is a fundamental sensory mechanism for every species and provides organisms with a detailed view of the environment. This review focuses on what is known of the neuronal and molecular substrates for thermosensation across species, focusing on the three robust model systems extensively used to study sensory signaling, the nematode Caenorhabditis elegans, the fruit fly Drosophila melanogaster, and the laboratory mouse. Nematodes migrate to thermal climes that are amenable to their survival, a behavior that is regulated primarily through a single sensory neuron. Additionally, nematodes "learn" to seek out this temperate zone based upon their prior experience, a robust model of learning and memory. Drosophila larvae also prefer select thermal zones that are optimal for growth and have also developed vigorous mechanisms to avoid unfavorable conditions. In mammals, the transduction mechanisms for thermosensation have been identified primarily due to the fact that naturally occurring plant products evoke distinct psychophysical sensation of temperature change. More remarkably, the elucidation of the molecular sensors in mammals, along with those in Drosophila, has demonstrated conservation in the molecular mediators of temperature sensation across diverse species.

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Section: Mouse Models Deficient In Temperature Sensingmentioning

confidence: 90%

Section: Mouse Models Deficient In Temperature Sensingmentioning

confidence: 91%

Section: Mouse Models Deficient In Temperature Sensingmentioning

confidence: 99%

See 1 more Smart Citation

Temperature sensing across species

McKemy

2007

Pflugers Arch - Eur J Physiol

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“…2000) and Dr David Corey (Kwan et al. 2006) and continuously backcrossed to C57BL/6 in our animal facility. The mice were housed in group cages in a temperature‐controlled environment on a 12 h light/dark cycle and were supplied with water and food ad libitum.…”

Section: Methodsmentioning

confidence: 99%

Taurolidine and congeners activate hTRPA1 but not hTRPV1 channels and stimulate CGRP release from mouse tracheal sensory nerves

Kichko

Pfirrmann

Reeh

2016

Pharmacology Res & Perspec

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Taurolidine has long been in clinical use as an antimicrobial irrigation that does not impede wound healing. It can even be administered intravenously (30 g/day) to treat sepsis or to exert newly recognized antineoplastic actions. Only one irritant effect is reported, that is, to temporarily induce burning pain of unknown origin when applied to body cavities or peripheral veins. The structure of the molecule suggested the chemoreceptor channel TRPA1 as a potential target, which was verified measuring stimulated CGRP release from sensory nerves of the isolated mouse trachea and calcium influx in hTRPA1‐transfected HEK293 cells. With both methods, the concentration–response relationship of taurolidine exceeded the threshold value below 500 μmol/L and 100 μmol/L, respectively, and reached saturation at 1 mmol/L. The clinical 2% taurolidine solution did not evoke greater or longer lasting responses. The reversible tracheal response was abolished in TRPA1−/− but retained in TRPV1−/− mice. Consistently, hTRPV1‐HEK showed no calcium influx as a response, likewise native HEK293 cells and hTRPA1‐HEK deprived of extracellular calcium did not respond to taurolidine 1 mmol/L. The metabolite taurultam and its oxathiazine derivative, expected to cause less burning pain, showed weak tracheal irritancy only at 10 mmol/L, acting also through hTRPA1 but not hTRPV1. In conclusion, taurolidine, its metabolite, and a novel derivative showed no unspecific cellular effects but selectively, concentration‐dependently and reversibly activated the irritant receptor TRPA1 in CGRP‐expressing, thus nociceptive, neurons. The clinical solution of 2% taurolidine (~70 mmol/L) can, thus, rightly be expected to cause transient burning pain and neurogenic inflammation.

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“…Another notable TRP subunit, TRPA1, gained considerable attention when it was presented as a candidate for the mechanosensitive transduction channel (Corey et al 2004). However, it was later ruled out as a candidate based on the lack of an auditory or vestibular deficit in TRPA1-deficient mice (Bautista et al 2006;Kwan et al 2006). To our surprise, TRPA1 RNA expression spiked over 100-fold in the apex at P0 and P2 relative to the base at P4.…”

Section: Trp Subunits With No or Little Expression And/or No Distinctmentioning

confidence: 99%

A Quantitative Analysis of the Spatiotemporal Pattern of Transient Receptor Potential Gene Expression in the Developing Mouse Cochlea

Asai

Holt

Géléoc

2009

JARO

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TRP genes encode a diverse family of ion channels which have been implicated in many sensory functions. Because several TRP channels have similar properties to the elusive hair cell transduction channel, recent attention has focused on TRP gene expression in the inner ear. At least four TRP genes are known to be expressed in hair cells: TRPC3, TRPV4, TRPA1, and TRPML3. However, there is little evidence supporting any of these as a component of the transduction complex. Other less well-characterized TRP channels are expressed in the inner ear, in particular, within the organ of Corti. Because of their potential role in sensory function, we investigated the developmental expression of RNA that encodes all 33 TRP subunits as well as several splice variants. We designed a quantitative PCR screen using cochlear samples acquired before, during, and after the time when mechanotransduction is acquired in sensory hair cells (embryonic day 17 to postnatal day 8). Cochleas, which included the organ of Corti, stria vascularis, and Reissner's membrane, were subdivided into four equal quadrants which allowed for regional comparison during development. Expression of RNA transcripts that encoded 33 TRP subunits plus several splice forms and beta-actin were quantified in 28 samples for a total of 1,092 individual measurements, each done in triplicate. We detected RNA that encoded all TRP channels except two: TRPC7 and TRPM8. The largest changes in RNA expression were for TRPA1 (9100-fold), which suggested that these subunits may contribute to normal cochlear function. Furthermore, the screen revealed TRPP3 and PKD1L3 RNA expression patterns that were correlated with the acquisition of sensory transduction in outer hair cells (Lelli et al., J Neurophysiol. 101:2961-2973, 2009). Numerous spatiotemporal expression gradients were identified many of which may contribute to the normal functional development of the mouse cochlea.

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TRPA1 Contributes to Cold, Mechanical, and Chemical Nociception but Is Not Essential for Hair-Cell Transduction

Cited by 1,144 publications

References 50 publications

Temperature sensing across species

Temperature sensing across species

Taurolidine and congeners activate hTRPA1 but not hTRPV1 channels and stimulate CGRP release from mouse tracheal sensory nerves

A Quantitative Analysis of the Spatiotemporal Pattern of Transient Receptor Potential Gene Expression in the Developing Mouse Cochlea

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