TRPV2 regulates BAT thermogenesis and differentiation

Sun, Wuping; Uchida, Kunitoshi; Tominaga, Makoto

doi:10.1080/19336950.2016.1228401

Cited by 21 publications

(26 citation statements)

References 8 publications

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“…Initially, TRPV2 augments Ca 2ϩ influx into brown preadipocytes, which releases repression of PPAR␥ by calcineurin and leads to BAT differentiation. Subsequently, TRPV2 continues mediating Ca 2ϩ influx in mature brown adipocytes, leading to the upregulation of UCP1 and enhancement in BAT function (29). In several aspects, our findings on TRPC1 parallel those observed for TRPV2.…”

Section: Discussionsupporting

confidence: 81%

“…TRPC1 containing complexes offer an attractive possibility of regulating PPAR␥ by modulating Ca 2ϩ levels. In white preadipocytes, increase of intracellular Ca 2ϩ levels leads to lowering of PPAR␥ levels and inhibition of white adipocyte differentiation (25a), whereas the relief of calcineurin repression of PPAR␥ by Ca 2ϩ influx mediated by TRPV2 drives the differentiation of BAT (29).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, TRPV2 knockout mice showed a number of metabolic defects consistent with BAT dysfunction, such as downregulation of UCP1 and impaired response to cold treatment (28). Subsequently TRPV2 was proposed to play a role in BAT differentiation by activation expression of PPAR␥ (29). Spurred by our initial observation that TRPC1 is highly expressed in adipocyte deposits but especially in BAT, we have characterized the metabolic phenotype of Trpc1 Ϫ/Ϫ mice as well as investigated the influence of TRPC1 downregulation on metabolic function and the expression profiles of key metabolic genes in cultured adipocytes.…”

Section: Introductionmentioning

confidence: 99%

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TRPC1 regulates brown adipose tissue activity in a PPARγ-dependent manner

Wolfrum

Kiehlmann

Pelczar

2018

American Journal of Physiology-Endocrinology and Metabolism

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Brown adipose tissue (BAT) has the unique ability of converting energy stored in the form of triglycerides into heat. This property makes BAT a target tissue to increase energy expenditure and improve systemic metabolic control. TRPC1 is a founding member of the TRP protein family that also includes several temperature-sensitive channels. We show that TRPC1 is highly expressed in all adipocyte depots, including BAT, and that Trpc1-deficient mice are prone to weight gain and manifest reduced metabolic control. We also demonstrate that knockdown of TRPC1 in cultured brown adipocytes leads to a downregulation of several metabolic genes, including UCP1 and PPARγ, as well as upregulation of the BAT-specific thermosensitive channel TRPV2, ultimately resulting in impaired respiratory function. We also show that TRPC1 is a possible target of PPARγ, suggesting that TRPC1 is a downstream component of a mechanism that translates metabolic or environmental stimuli into output in the form of BAT activity. Better understanding of the possible role of TRPC1 and other TRP channels in body temperature regulation and BAT function may help us to develop obesity therapies based on BAT activation.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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TRPC1 regulates brown adipose tissue activity in a PPARγ-dependent manner

Wolfrum

Kiehlmann

Pelczar

2018

American Journal of Physiology-Endocrinology and Metabolism

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“…These findings suggested that the TRPV2-mediated Ca 2+ influx plays an important role in BAT differentiation and thermogenesis. And TRPV2 could be a target for preventing human obesity and other metabolic-related diseases [77][78][79]. However, the detailed mechanisms of TRPV2 in adipocyte differentiation are still unknown, which needs further studies in the future.…”

Section: Trpv Family In Adipogenesismentioning

confidence: 99%

Involvement of calcium channels in the regulation of adipogenesis

Zhai

Yang

et al. 2020

Adipocyte

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As an important second messenger in adipocytes, calcium ions (Ca 2+) are essential in regulating various intracellular signalling pathways that control critical cellular functions. Calcium channels show selective permeability to Ca 2+ and facilitate Ca 2+ entry into the cytoplasm, which are normally located in the plasmatic and intracellular membranes. The increase of cytosolic Ca 2+ modulates a variety of signalling pathways and results in the transcription of target genes that contribute to adipogenesis, a key cellular event includes proliferation and differentiation of adipocyte. In the past decades, the involvement of some Ca 2+-permeable ion channels, such as Ca 2+ release-activated Ca 2+ channels, transient receptor potential channels, voltage-gated calcium channels and others, in adipogenesis has been extensively explored. In the present review, we provided a summary of the expression and contributions of these Ca 2+-permeable channels in mediating Ca 2+ influxes that drive adipogenesis. Moreover, we discussed their potentials as future therapeutic targets.

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“…The insult of GSK1016790A to A375 cells led to an acute increase in cellular temperature (about 0.15 C), which was maintained during the experimental period, while HC-067047 led to a decrease in cellular temperature (by about 0.2 C), which was also sustained during the experimental period. Since calcium ions are reported to be associated with the changes in cell temperature (Sun et al 2017), and TRPV4 are reported as non-selective ion channel proteins which were mainly involved in a plethora of Ca 2þ -mediated cell functions, the cellular calcium influx evoked by the drug was examined in this study. From the results, it can be seen that GSK1016790A caused a calcium influx in A375 cells (Figure 3(G,H)), while this effect failed to be observed when HC-067047 was pre-incubated in advance (Figure 3(G, I)).…”

Section: Sem Observation Of A375 Cells Grown On Thermal Resistorsmentioning

confidence: 99%

Exploring the ‘cold/hot’ properties of traditional Chinese medicine by cell temperature measurement

Ding

et al. 2020

Pharmaceutical Biology

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Context: It is common sense that chewing a mint leaf can cause a cooling feeling, while chewing ginger root will produce a burning feeling. In Traditional Chinese Medicine (TCM), this phenomenon is referred to as 'cold/hot' properties of herbs. Herein, it is reported that TCM with different "cold/hot" properties have different effects on the variation of cells. Objective: To explore the intrinsic 'cold/hot' properties of TCM from the perspective of cellular and molecular biology. Materials and methods: A375 cells were selected using Cancer Cell Line Encyclopaedia (CCLE) analysis and western blots. Hypaconitine and baicalin were selected by structural similarity analysis from 56 and 140 compounds, respectively. A wireless thermometry system was used to measure cellular temperature change induced by different compounds. Alteration of intracellular calcium influx was investigated by means of calcium imaging. Results: The IC 50 values of GSK1016790A, HC067047, hypaconitine, and baicalin for A375 cells are 8.363 nM, 816.4 lM, 286.4 lM and 29.84 lM, respectively. And, 8 lM hypaconitine induced obvious calcium influx while 8 lM baicalin inhibited calcium influx induced by TRPV4 activation. Cellular temperature elevated significantly when treated with GSK1016790A or hypaconitine, while the results were reversed when cells were treated with HC067047 or baicalin. Discussion and conclusions: The changes in cellular temperature are speculated to be caused by the alteration of intracellular calcium influx mediated by TRPV4. In addition, the 'cold/hot' properties of compounds in TCM can be classified by using cellular temperature detection.

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TRPV2 regulates BAT thermogenesis and differentiation

Cited by 21 publications

References 8 publications

TRPC1 regulates brown adipose tissue activity in a PPARγ-dependent manner

TRPC1 regulates brown adipose tissue activity in a PPARγ-dependent manner

Involvement of calcium channels in the regulation of adipogenesis

Exploring the ‘cold/hot’ properties of traditional Chinese medicine by cell temperature measurement

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