This study puts forth the following research questions: (i) Are there any differences between remotes' and co-locates' knowledge portfolios, and (ii) if so, are these differences significant? We contend that significant differences occur in how remotes configure their knowledge activities. To search for possible differences, we examined three sources of knowledge-based activities that are associated with remotes and co-locates: university collaborations and innovative activities such as patenting and scanning. Using survey data from a study of 197 U.S. biotechnology firms, this study found that remotes and co-locates have similar portfolio diversity, but remotes sponsor more faculty research, have a lower patent efficiency rate, and do not scan as frequently as co-located firms.