Trypanosoma congolense: Surface glycoproteins of two early bloodstream variants

Rosen, N; Onodera, Manabu; Hotez, Peter J.; Bogucki, Mary S.; Elce, B.J.; Patton, Curtis L.; Konigsberg, William H.; Cross, George A.M.; Richards, Frederic M.

doi:10.1016/0014-4894(81)90076-x

Cited by 12 publications

(4 citation statements)

References 6 publications

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“…The absence of free bradyzoites along with the isolated cysts was confirmed initially by microscopy and subsequently by the lack of infection in wells to which the remaining drops of medium were added. A similar concept has been used for cloning trypanosomes in mice (Rosen et al 1981).…”

Section: Discussionmentioning

confidence: 96%

In vitro generation of cloned populations of Toxoplasma gondii

Sreekumar

Rao

Mishra

et al. 2003

Parasitology Research

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Cloned populations were generated from Indian isolates of Toxoplasma gondii by transferring single tissue cysts from the brains of chronically infected mice to confluent murine macrophage (J774A.1) monolayers. The clones were then maintained continuously as tachyzoites in culture. Physical rupture of the tissue cysts and release of bradyzoites prior to seeding was found to be necessary for establishment of the parasite in culture. Although intact tissue cysts seeded over monolayers released bradyzoites spontaneously, they did not succeed in setting up an infection in the monolayers. Random amplified polymorphic DNA (RAPD)-PCR, which revealed distinct patterns for a clone and its progenitor, further confirmed the efficiency of the technique. The cloning technique was found to be simple and rapid compared to those involving limiting dilutions.

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Section: Discussionmentioning

confidence: 96%

In vitro generation of cloned populations of Toxoplasma gondii

Sreekumar

Rao

Mishra

et al. 2003

Parasitology Research

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“…Amongst several methods described for isolation of surface constituents (GLICK, 1974), the one based on the use of proteolytic enzymes was adopted in the present study. Surprisingly, trypsin which has been successfully used with mammalian cells (GLICK, 1974) as well as with a few parasites also (CROSS, 1975;ROSEN et al, 1981;TURNER & GREGSON, 1982), was found unsatisfactory with A. galli. The reason for this failure may be attributed to the inactivation of the enzyme by its inhibitor, shown to be possessed (ANSARI et al, 1976) and released into the medium (Srivastava, V. M. L., Unpublished data) by A. galli.…”

Section: Resultsmentioning

confidence: 99%

“…Characterization of these entities in a number of parasites has been carried out with modern probes such as the binding of lectins (MURRELL et al, 1978;SIMPSON & SMITHERS, 1980;FURMAN & A S H , 1983;DEVANEY, 1985), electrophoresis following radio-iodination (ABBAS & CAIN, 1980;PHILLIP et al, 1980PHILLIP et al, , 1984SNARY et al, 1980;BASCHONG et al, 1982) and staining with dyes (TRACY & MAHMOUD, 1982;HIMMELHOCH & ZUCKERMAN, 1983;GOLDBERG et al, 1983). However, regarding the isolation and purification of these molecules which may provide the requisite information for developing effective immunoprophylactic measures, only a few reports are available (CROSS, 1975;Dissous et al, 1981;OLENICK et al, 1981;ROSEN et al, 1981;BASCHONG et al, 1982;GOLDBERG et al, 1983;PHILIPP & RUMJANECK, 1984). In order to provide more information in this area, the present investigation deals with the purification and characterization of a glycoprotein from the cuticular surface of Ascaridia galli, an intestinal nematode of the fowl.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a glycoprotein from the surface ofAscaridia galli

Masood

Sircar²,

Srivastava

1987

J. Helminthol.

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Employing papain as the enzyme and agarose bound Ricinus communis agglutinin as the affinity gel, a glycoprotein has been isolated and purified from the surface of Ascaridia galli. The glycoprotein shows an apparent molecular weight of 68 kilo daltons and contains fucose, galactose, rhamnose and glucosamine as sugar moieties. Only 2% of its entire molecule has been found to possess α-helical configuration.

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“…The trypanosome populations tested were derived from single cloned parasites prepared by the method of Rosen et al (1981). Each clone causes fatal parasitaemia.…”

Section: Methodsmentioning

confidence: 99%

Direct and Sensitive Detection of Trypanosoma Evansi by Polymerase Chain Reaction

Omanwar

Rao

Butchaiah³

et al. 1999

Acta Veterinaria Hungarica

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The mechanically transmitted haemoflagellate, Trypanosoma evansi causes surra, a wasting disease of domestic animals and is highly endemic in distribution in Southeast Asia. The detection of T. evansi is important for improving the epizootiological and animal health status of the region. The specificity and sensitivity of polymerase chain reaction (PCR) using oligonucleotide primers constructed from T. evansi repetitive DNA sequences were studied in the present investigation. Using the assay, it was possible to amplify template DNA of T. evansi derived from buffaloes, camels and horses to a threshold sensitivity level of 0.5 pg and to detect DNA from as few as five organisms in 10 µl crude blood samples.

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Trypanosoma congolense: Surface glycoproteins of two early bloodstream variants

Cited by 12 publications

References 6 publications

In vitro generation of cloned populations of Toxoplasma gondii

In vitro generation of cloned populations of Toxoplasma gondii

Purification and characterization of a glycoprotein from the surface ofAscaridia galli

Direct and Sensitive Detection of Trypanosoma Evansi by Polymerase Chain Reaction

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