Tryptophan hydroxylase is modulated by L-type calcium channels in the rat pineal gland

Barbosa, Roseli; Scialfa, Julieta Helena; Terra, Ilza Mingarini; Cipolla‐Neto, José; Simonneaux, Valérie; Afeche, Solange Castro

doi:10.1016/j.lfs.2007.12.011

Cited by 29 publications

(20 citation statements)

References 37 publications

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“…Tryptophan hydroxylase (TPH) activity was quantified as previously described [41]. Briefly, each tissue sample was sonicated in sodium phosphate buffer (2 mM, pH 7, 100 μL), and the following mixture was added to each sample: HEPES (50 mM, pH 7), catalase (100 μg ml -1 ), tryptophan (50 μM), dithiothreitol (5 mM), Fe (NH 4 ) 2 (SO 4 ) 2 (10 μM), 6-MPH4 (500 μM) and 1 μL of [3 H ] tryptophan (1 mCi ml -1 - previously dried under nitrogen).…”

Section: Methodsmentioning

confidence: 99%

Developmental and light-entrained expression of melatonin and its relationship to the circadian clock in the sea anemone Nematostella vectensis

Peres

Reitzel

Passamaneck

et al. 2014

EvoDevo

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BackgroundThe primary hormone of the vertebrate pineal gland, melatonin, has been identified broadly throughout the eukaryotes. While the role for melatonin in cyclic behavior via interactions with the circadian clock has only been reported in vertebrates, comparative research has shown that the transcription-translation loops of the animal circadian clock likely date to the cnidarian-bilaterian ancestor, leaving open significant questions about the evolutionary origin of melatonin signaling in circadian behavior by interacting with the molecular clock.ResultsExpression of melatonin in adult anemones showed peak expression at the end of light period (zeitgeber time (ZT) = 12) when cultured under diel conditions, coinciding with expression of genes and enzyme activity for members of the melatonin synthesis pathway (tryptophan hydroxylase and hydroxyindol-O-methyltransferase), which also showed rhythmic expression. During embryogenesis and juvenile stages, melatonin showed cyclic oscillations in concentration, peaking in midday. Spatial (in situ hybridization) and quantitative (real-time PCR) transcription of clock genes during development of N. vectensis showed these ‘clock’ genes are expressed early in the development, prior to rhythmic oscillations, suggesting functions independent of a function in the circadian clock. Finally, time-course studies revealed that animals transferred from diel conditions to constant darkness lose circadian expression for most of the clock genes within 4 days, which can be reset by melatonin supplementation.ConclusionsOur results support an ancient role for melatonin in the circadian behavior of animals by showing cyclic expression of this hormone under diel conditions, light-dependent oscillations in genes in the melatonin synthesis pathway, and the function of melatonin in initiating expression of circadian clock genes in the cnidarian N. vectensis. The differences in expression melatonin and the circadian clock gene network in the adult stage when compared with developmental stages of N. vectensis suggests new research directions to characterize stage-specific mechanisms of circadian clock function in animals.

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Section: Methodsmentioning

confidence: 99%

Developmental and light-entrained expression of melatonin and its relationship to the circadian clock in the sea anemone Nematostella vectensis

Peres

Reitzel

Passamaneck

et al. 2014

EvoDevo

Self Cite

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“…The TRP concentration in the sonicate samples was quantified using a microplate fluorometer (Ascent, Labsystems) with appropriate settings (excitation 485 nm/emission 680 nm) and expressed as nmol per pineal gland. and 10% methanol for 5-HTP, 5-HT and NAS, or 30% methanol for MEL [4]. The flow rate was 1 ml/min and the electrochemical detector gold electrode potential was set at +0.9 V against the Ag/AgCl reference electrode.…”

Section: Measurement Of Trp the Pineal Mel Substratementioning

confidence: 99%

Pineal arylalkylamine N-acetyltransferase (Aanat) gene expression as a target of inflammatory mediators in the chicken

Piesiewicz

Kędzierska

Adamska

et al. 2012

General and Comparative Endocrinology

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“…8A), the k 0 values of NT decreased when the concentration of NF increased. NT and NF are calcium antagonists [41] that can selectively interact with the L-CCRs abundant in the VSM-cell membrane. As such, the greater the number of active points of L-CCRs on the stationary phase that were occupied by NF in the mobile phase, then the weaker the binding between NT and L-CCRs.…”

Section: Interactions Between Nt Sta and Dsd And Vsm-cell Membrane Rmentioning

confidence: 99%

Screening vasoconstriction inhibitors from traditional Chinese medicines using a vascular smooth muscle/cell membrane chromatography‐offline‐liquid chromatography‐mass spectrometry

Yang

Wang

Zhang

et al. 2011

J of Separation Science

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We developed an analytical method for screening vasoconstriction inhibitors from traditional Chinese medicines (TCMs) by combining vascular smooth muscle/cell membrane chromatography (VSM/CMC) with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Primary cultured VSM cells from rat thoracic aortas were used for preparation of the stationary phase of the VSM/CMC column. Retention fractions from the VSM/CMC column were collected and then analyzed by LC-MS/MS under the optimized conditions offline. The suitability and reliability of the VSM/CMC-offline-LC-MS/MS method was assessed using nitrendipine and nifedipine as positive controls, and this method was then applied to screen vasodilator components from the extracts of Fructus Schisandrae Chinensis (FSC) and Fructus Schisandrae Sphenantherae (FSS). The major components from both species retained by VSM/CMC were identified as deoxyschizandrin (DSD) and schisantherin A (STA) by LC-MS/MS. Competition experiments indicated that DSD and nifedipine bound competitively to membrane receptors, while DSD and STA had partly overlapping binding sites on VSM-cell membranes. In vitro pharmacological trials confirmed that STA and DSD could dose-dependently relax the rat thoracic aortas pre-contracted by KCl. Our VSM/CMC-offline-LC-MS/MS method can be applied for screening vasoconstriction inhibitors from TCMs collected from FSC and FSS, and may be useful in the development of vasodilators from natural products.

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Tryptophan hydroxylase is modulated by L-type calcium channels in the rat pineal gland

Cited by 29 publications

References 37 publications

Developmental and light-entrained expression of melatonin and its relationship to the circadian clock in the sea anemone Nematostella vectensis

Developmental and light-entrained expression of melatonin and its relationship to the circadian clock in the sea anemone Nematostella vectensis

Pineal arylalkylamine N-acetyltransferase (Aanat) gene expression as a target of inflammatory mediators in the chicken

Screening vasoconstriction inhibitors from traditional Chinese medicines using a vascular smooth muscle/cell membrane chromatography‐offline‐liquid chromatography‐mass spectrometry

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