Tryptophan residue is essential for immunoreactivity of a diagnostically relevant peptide epitope of A. fumigatus

Kamal, Neel; Chowdhury, Shantanu; Madan, Taruna; Sharma, Deepak; Attreyi, M.; Haq, W.; Katti, S. B.; Kumar, Anil; Sarma, P. Usha

doi:10.1007/s11010-005-2056-x

Cited by 5 publications

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“…One of the earliest known and characterized allergen/antigen of Afu is Asp f1 with 85% of allergic aspergillosis patients showing IgE antibodies to Asp f1 (17,18,23-25). Asp f1 is a cytotoxic ribonuclease hindering its use for immunotherapy in a native form owing to the possible toxicities and IgE-mediated side effects.…”

Section: Introductionmentioning

confidence: 99%

Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

et al. 2009

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BackgroundThe present study examines a hypothesis that short allergen-derived peptides may shift an Aspergillus fumigatus (Afu-) specific TH2 response towards a protective TH1. Five overlapping peptides (P1-P5) derived from Asp f1, a major allergen/antigen of Afu, were evaluated for prophylactic or therapeutic efficacy in BALB/c mice.MethodsTo evaluate the prophylactic efficacy, peptides were intranasally administered to naïve mice and challenged with Afu-allergens/antigens. For evaluation of therapeutic efficacy, the mice were sensitized with Afu-allergens/antigens followed by intranasal administration of peptides. The groups were compared for the levels of Afu-specific antibodies in sera and splenic cytokines evaluated by ELISA. Eosinophil peroxidase activity was examined in the lung cell suspensions and lung inflammation was assessed by histopathogy.ResultsPeptides P1-, P2- and P3 decreased Afu-specific IgE (84.5~98.9%) and IgG antibodies (45.7~71.6%) in comparison with Afu-sensitized mice prophylactically. P1- and P2-treated ABPA mice showed decline in Afu-specific IgE (76.4~88%) and IgG antibodies (15~54%). Increased IgG2a/IgG1 and IFN-γ/IL-4 ratios were observed. P1-P3 prophylactically and P1 therapeutically decreased IL-5 levels and eosinophil peroxidase activity. P1 decreased inflammatory cells' infiltration in lung tissue comparable to non-challenged control.ConclusionAsp f1-derived peptide P1, prophylactically and therapeutically administered to Balb/c mice, is effective in regulating allergic response to allergens/antigens of Afu, and may be explored for immunotherapy of allergic aspergillosis in humans.

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Section: Introductionmentioning

confidence: 99%

Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

et al. 2009

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“…Using an assay with these proteins mutated at their residues important for IgE binding, they showed that the protein specifically lost its binding activity and exhibited reduced IgE induction in the immunized mice. Kamal et al (2005) described that the tryptophan residue is essential for immunoreactivity of a diagnostically relevant peptide epitope of A. fumigatus. The loss of specific IgG and IgE antibody binding of the modified protein by ELISA confirmed the critical role of tryptophan (Trp17) in the immunoreactivity of this protein.…”

Section: Solutionsmentioning

confidence: 99%

Allergens and Toxins from Oleaginous Plants: Problems and Solutions

Oliveira¹,

Machado²

2011

Environmental Impact of Biofuels

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Survey of the year 2005 commercial optical biosensor literature

2006

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We identified 1113 articles (103 reviews, 1010 primary research articles) published in 2005 that describe experiments performed using commercially available optical biosensors. While this number of publications is impressive, we find that the quality of the biosensor work in these articles is often pretty poor. It is a little disappointing that there appears to be only a small set of researchers who know how to properly perform, analyze, and present biosensor data. To help focus the field, we spotlight work published by 10 research groups that exemplify the quality of data one should expect to see from a biosensor experiment. Also, in an effort to raise awareness of the common problems in the biosensor field, we provide side-by-side examples of good and bad data sets from the 2005 literature.

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Tryptophan residue is essential for immunoreactivity of a diagnostically relevant peptide epitope of A. fumigatus

Cited by 5 publications

References 31 publications

Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

Allergens and Toxins from Oleaginous Plants: Problems and Solutions

Survey of the year 2005 commercial optical biosensor literature

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