TSH-induced differentiated functions correlate with enhancement of phosphotyrosine phosphatase activity in thyroid cells. Effect of phorbol 12-myristate 13-acetate

Petitfrere, E.; Huet, Eric; Sartelet, Hervé; Martiny, Laurent; Legué, Odette; Haye, Bernard

doi:10.1677/joe.0.1690603

Cited by 3 publications

(2 citation statements)

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“…Previously published in vivo and in vitro stimulation experiments have been performed using isolated thyroid follicular epithelial cells. Upon incubation of these cells in media containing the native form of TSHβ, increased iodide transport activity, TG iodination, protein synthesis and phospholipid synthesis were observed (16). The results of the in vivo and in vitro experiments performed in the present study confirm that the TSHβ splice variant may also contribute to the synthesis and secretion of thyroid hormones, which is similar to the function of the native form of TSHβ.…”

Section: Discussionsupporting

confidence: 74%

HPT axis‑independent TSHβ splice variant regulates the synthesis of thyroid hormone in mice

et al. 2019

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Thyroid stimulating hormone (TSH) consists of an α-subunit and a unique β-subunit. The first in-frame TSHβ splice variant produced by the cells of immune system was identified in 2009. The TSHβ splice variant and native TSHβ exhibit different expression profiles, and research has been conducted to elucidate the role of the TSHβ splice variant in different diseases. However, understanding of the fundamental physiological characteristics of the TSHβ splice variant is currently limited. To verify whether the TSHβ splice variant has the potential to induce thyroid follicular cells to synthesize thyroid hormone, in vivo and in vitro stimulation experiments were conducted in the present study. A total of 60 C57BL/6 mice were divided into control-, 5 and 10 µg TSHβ splice variant-treated groups at random. Mice were sacrificed at 0.5, 1 and 4 h after intraperitoneal injection, and serum levels of tri-iodothyronine (T3) and thyroxine (T4) were determined using a radioimmunoassay. Thyroid follicular cells were isolated from the thyroids of mice, and stimulated with 2 µg/ml TSHβ splice variant. Supernatants were collected, and the levels of T3 and T4 were detected. The protein expression levels of the sodium-iodide symporter, thyroperoxidase and thyroglobulin in thyroid follicular cells were quantified using western blot analysis. To verify whether the TSHβ splice variant expression was regulated by the hypothalamus-pituitary-thyroid (HPT) axis, similar to native TSHβ, a total of 60 C57BL/6 mice were equally divided into control, 2 mg/kg T3 intraperitoneal injection and 0.05 mg/kg thyroid-releasing hormone intraperitoneal injection groups at random. Mice were sacrificed at 1 and 4 h after injection. Alterations in the expression of the TSHβ splice variant in the pituitary, thyroid, peripheral blood leukocytes and spleen tissues were detected using western blot analysis. The present study demonstrated that the TSHβ splice variant is not regulated by the HPT axis and may affect thyroid hormone synthesis. Modifications in the expression of the TSHβ splice variant may occur in a uniquely regulated manner to provide peripheral immunological compartments with a source of activated cells, particularly under immune stress.

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Section: Discussionsupporting

confidence: 74%

HPT axis‑independent TSHβ splice variant regulates the synthesis of thyroid hormone in mice

et al. 2019

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“…Many supplements, including thyroid-stimulating hormone (TSH), bovine insulin, transferrin, and L -glutamine, were added to the culture medium to promote cell proliferation and avoid differentiation as previously described ( Curcio et al, 1994 ). A previous study showed that TSH stimulated primary cultures of human thyroid cells ( Kogai et al, 2000 ; Petitfrere et al, 2001 ). In this study, TSH was essential for the adherence and proliferation of the hTERT-BTY cell line and the hTERT-BTY cells lost adhesive capacity when the medium lacked bovine TSH.…”

Section: Discussionmentioning

confidence: 99%

Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus

et al. 2018

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Foot-and-mouth disease virus (FMDV) has a wide host range. Its pathogenesis varies among hosts and types of viruses. Most investigations of pathogenesis have been performed on cattle and swine. However, FMDV research in cattle is hampered by the lack of a stable in vitro infection model. In this study, the stable bovine thyroid (BTY) cell line hTERT-BTY from primary BTY cells was established by telomerase reverse transcriptase over expression. The results of karyotype analysis and experiments on morphological and biological characteristics indicated that this cell line possessed the qualities of primary BTY cells, which could be extended indefinitely with stable morphology and steady growth rates. The hTERT-BTY cell line, has 60 chromosomes including 29 pairs of autosomes and 1 pair of sex chromosomes without structure aberrations. It can express thyroid-specific function genes thyroid-stimulating hormone receptor and sodium/iodide symporter in high abundance ratios. The cell line is sensitive to FMDV strains and is expected to be used as a powerful tool for FMDV clinical diagnosis, separation, detection and culture. Also, the different mRNA expression levels in infected and uninfected hTERT-BTY cells were analyzed in this study to identify the pathways of immunity using RNA-seq. The results suggested that the hTERT-BTY cell line could be regarded as an effective tool for the immune response exploration of FMDV. In conclusion, this study provided a useful tool for FMDV clinical diagnosis, separation, detection, and culture. The cell line also could serve as an in vitro model to study the mechanism underlying FMDV pathogenicity and host–virus interaction.

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Activation of Protein Kinase CδInduces Growth Arrest in NPA Thyroid Cancer Cells Through Extracellular Signal-Regulated Kinase Mitogen-Activated Protein Kinase

Koike

Fujii

Nakamura

et al. 2006

Thyroid

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Protein kinase C (PKC) is a family of serine-threonine kinases that regulate many cell processes. To study the role of PKCdelta in thyroid cancer cells, we used a replication-deficient adenovirus (PKCdeltaAdV), to tightly control PKCdelta expression. In NPA cells, activation of wild-type (WT) PKCdelta with phorbol 12-myristate 13-acetate (PMA) induced an arrest in cell growth at G(1) phase, which was itself inhibited by the PKCdelta inhibitor rottlerin. Furthermore, overexpression of a dominant negative PKCdelta did not induce G(1) arrest. These findings strongly suggested that PKCdelta induced cell growth arrest in NPA cells. We investigated the mechanism of G1 arrest by examining G(1)-related proteins and mitogen-activated protein kinase (MAPK) by Western blotting. After activation of WTPKCdelta with PMA, cyclin E expression and retinoblastoma protein (Rb) phosphorylation decreased; the expression of p27(Kip1) increased and the phosphorylation of extracellular signal-regulated kinase (ERK) MAPK decreased. These results indicated that the activation of PKCdelta induced cell growth arrest in NPA cells, through an ERK MAPK-p27(Kip1)-cyclin E-pRb pathway. PKCdelta may therefore be an effective molecular target for novel therapy in thyroid cancer.

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TSH-induced differentiated functions correlate with enhancement of phosphotyrosine phosphatase activity in thyroid cells. Effect of phorbol 12-myristate 13-acetate

Cited by 3 publications

References 34 publications

HPT axis‑independent TSHβ splice variant regulates the synthesis of thyroid hormone in mice

HPT axis‑independent TSHβ splice variant regulates the synthesis of thyroid hormone in mice

Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus

Activation of Protein Kinase CδInduces Growth Arrest in NPA Thyroid Cancer Cells Through Extracellular Signal-Regulated Kinase Mitogen-Activated Protein Kinase

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