TsPAP1 encodes a novel plant prolyl aminopeptidase whose expression is induced in response to suboptimal growth conditions

Szawłowska, Urszula; Grabowska, Agnieszka; Zdunek‐Zastocka, Edyta; Bielawski, W

doi:10.1016/j.bbrc.2012.01.140

Cited by 15 publications

(6 citation statements)

References 33 publications

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“…Despite the ubiquity of PAP in nature, its role remains poorly understood in plants. To date, studies have only shown that the mRNA level of PAP is increased in the shoots of triticale plants in response to suboptimal conditions such as drought and high saline [52]. Our results are the first to indicate that PAP may function in plant growth and development, including the control of shoot apical meristem formation.…”

Section: Discussionmentioning

confidence: 50%

Multiple Classes of Immune-Related Proteases Associated with the Cell Death Response in Pepper Plants

et al. 2013

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Proteases regulate a large number of biological processes in plants, such as metabolism, physiology, growth, and defense. In this study, we carried out virus-induced gene silencing assays with pepper cDNA clones to elucidate the biological roles of protease superfamilies. A total of 153 representative protease genes from pepper cDNA were selected and cloned into a Tobacco rattle virus-ligation independent cloning vector in a loss-of-function study. Silencing of 61 proteases resulted in altered phenotypes, such as the inhibition of shoot growth, abnormal leaf shape, leaf color change, and lethality. Furthermore, the silencing experiments revealed that multiple proteases play a role in cell death and immune response against avirulent and virulent pathogens. Among these 153 proteases, 34 modulated the hypersensitive cell death response caused by infection with an avirulent pathogen, and 16 proteases affected disease symptom development caused by a virulent pathogen. Specifically, we provide experimental evidence for the roles of multiple protease genes in plant development and immune defense following pathogen infection. With these results, we created a broad sketch of each protease function. This information will provide basic information for further understanding the roles of the protease superfamily in plant growth, development, and defense.

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Section: Discussionmentioning

confidence: 50%

Multiple Classes of Immune-Related Proteases Associated with the Cell Death Response in Pepper Plants

et al. 2013

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“…Albeit rare, protein-degrading enzymes were unique in asymptomatic needles compared to symptomatic needles which could indicate plant's response to improve tolerance against stressors. Some (e.g., prolyl aminopeptidases) can be rapidly over-induced when plants are subject to unfavorable environment [90,91] and improve plant stress tolerance [92]. We also found enzymes (e.g., M41) that could function as response to light stress resulting in photosystem repair [93].…”

Section: Fungal Gene Expressions In the Lophodermella Needle Cast Pathosystemmentioning

confidence: 78%

Community and Transcriptome Foliar Mycobiota Transitions in Response to Pathogenic Conifer Needle Interactions

Ata

Caballero

Abdo

et al. 2022

Preprint

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Profiling the host-mycobiota interactions in healthy vs. diseased forest ecosystems helps understand the dynamics of understudied yet increasingly important threats to forest health that are emerging due to climate change. We analyzed the structural and functional changes of the mycobiota and the responses of Pinus contorta in the Lophodermella needle cast pathosystem through metabarcoding and metatranscriptomics. When needles transitioned from asymptomatic to symptomatic, dysbiosis of the mycobiota occurred, but with an enrichment of Lophodermella pathogens. Many pathogenicity-related genes were highly expressed by the mycobiota at the necrotrophic phase, suggesting an active pathogen response that are absent in asymptomatic needles. This study also revealed that Lophodermella spp. are members of a healthy needle mycobiota that have a latent lifestyles suggesting that other pine needle pathogens may have similar biology. Interestingly, P. contorta upregulated defense genes in healthy needles, indicating response to fungal recognition, while a variety of biotic and abiotic stresses genes were activated in diseased needles. Further investigation to elucidate the possible antagonistic interplay of other biotic members leading to disease progression and/or suppression is warranted. This study provides insights into microbial interactions in non-model pathosystems and contributes to the development of new forest management strategies against emerging latent pathogens.

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“…Proline/propyl amino peptidase (APP) hydrolyzes the peptide bond between any amino acid and a penultimate proline residue at the N terminal of oligopeptide and protein substrates. Recently, Triticale APP (TsPAP1) was reported to be expressed under drought, saline, cadmium and aluminium stress (Szawłowska et al 2012). Aldehyde reductase (ALR), member of aldo keto reductases superfamily of proteins detoxifies the degradation products of lipid peroxidation.…”

Section: Resultsmentioning

confidence: 99%

Expression analysis of drought stress specific genes in Peanut (Arachis hypogaea , L.)

Pruthvi

Rama

Govind

et al. 2012

Physiol Mol Biol Plants

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Improving drought tolerance through gene manipulation has been of importance for modern agriculture, which requires identification and validation of candidate genes. Prospecting candidate genes from drought adapted crop species is of immense significance. To identify candidate stress responsive genes from adapted crop, we carried out expression analysis of a few drought responsive ESTs from Arachis hypogaea L. (peanut). The expression patterns of nine AhDR (Arachis hypogea drought responsive) clones were analysed under drought. Quantitative reverse transcription PCR analysis revealed stress responsive nature of the selected genes. The clones AhDR 118 (putative cyclin T-like), AhDR185 (aldehyde reductase-like), AhDR193 (cholin kinase-like) and AhDR 76 (proline amino peptidase-like) showed more than five fold increase in expression. Highly upregulated genes analysed for expression pattern against salinity at seedling level indicated that these genes provide cross protection. This paper is the first report indicating the association of peanut genes cyclin T, proline amino peptidase and choline kinase to drought tolerance, and the possible roles of these genes are discussed.

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TsPAP1 encodes a novel plant prolyl aminopeptidase whose expression is induced in response to suboptimal growth conditions

Cited by 15 publications

References 33 publications

Multiple Classes of Immune-Related Proteases Associated with the Cell Death Response in Pepper Plants

Multiple Classes of Immune-Related Proteases Associated with the Cell Death Response in Pepper Plants

Community and Transcriptome Foliar Mycobiota Transitions in Response to Pathogenic Conifer Needle Interactions

Expression analysis of drought stress specific genes in Peanut (Arachis hypogaea , L.)

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