Tuber Vegetative Stages and Cell Cycle in Helianthus tuberosus: Protein Pattern and their Modification by Spermidine

Duca, Stefano Del; Creus, J. A.; D'Orazi, Dario; Dondini, Luca; Bregoli, Anna Maria; Serafini-Fracassini, Donatella

doi:10.1016/s0176-1617(00)80267-9

Cited by 23 publications

(13 citation statements)

References 30 publications

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“…Explants, when put in in vitro culture, immediately respond activating many metabolisms, included the biosynthetic and catabolic ones of PAs, their linkage to RNAs and their conjugation by TGase activity especially to very high molecular mass conjugates and to a 18-kDa protein. New TGase enzymes are synthesized either of 58 and 90 kDa but also of higher mass ( Serafini-Fracassini et al, 1988 ; Del Duca et al, 2000 ). Cells activate the metabolisms of DNA and of different RNAs, and enter the first synchronous amitotic cell cycle continuing to grow, as partially summarized by Bagni and Serafini-Fracassini (1985) and by Tassoni et al (2010) .…”

Section: Transglutaminase In Programmed Cell Deathmentioning

confidence: 99%

“…Among the high molecular mass bands, a 150-kDa band increased dramatically at dormancy release. Numerous additional bands in the 35–50 kDa range appeared only in this phase ( Del Duca et al, 2000 ). These changes could be related either to the synthesis of degradative enzymes and to released storage substances to be transferred to growing sprouts or to the PCD of tuber parenchyma, which has completed its function.…”

Section: Transglutaminase In Programmed Cell Deathmentioning

confidence: 99%

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Senescence and programmed cell death in plants: polyamine action mediated by transglutaminase

2014

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Research on polyamines (PAs) in plants laps a long way of about 50 years and many roles have been discovered for these aliphatic cations. PAs regulate cell division, differentiation, organogenesis, reproduction, dormancy-break and senescence, homeostatic adjustments in response to external stimuli and stresses. Nevertheless, the molecular mechanisms of their multiple activities are still matter of research. PAs are present in free and bound forms and interact with several important cell molecules; some of these interactions may occur by covalent linkages catalyzed by transglutaminase (TGase), giving rise to “cationization” or cross-links among specific proteins. Senescence and programmed cell death (PCD) can be delayed by PAs; in order to re-interpret some of these effects and to obtain new insights into their molecular mechanisms, their conjugation has been revised here. The TGase-mediated interactions between proteins and PAs are the main target of this review. After an introduction on the characteristics of this enzyme, on its catalysis and role in PCD in animals, the plant senescence and PCD models in which TGase has been studied, are presented: the corolla of naturally senescing or excised flowers, the leaves senescing, either excised or not, the pollen during self-incompatible pollination, the hypersensitive response and the tuber storage parenchyma during dormancy release. In all the models examined, TGase appears to be involved by a similar molecular mechanism as described during apoptosis in animal cells, even though several substrates are different. Its effect is probably related to the type of PCD, but mostly to the substrate to be modified in order to achieve the specific PCD program. As a cross-linker of PAs and proteins, TGase is an important factor involved in multiple, sometimes controversial, roles of PAs during senescence and PCD.

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Section: Transglutaminase In Programmed Cell Deathmentioning

confidence: 99%

Section: Transglutaminase In Programmed Cell Deathmentioning

confidence: 99%

Senescence and programmed cell death in plants: polyamine action mediated by transglutaminase

2014

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“…Polyamines (PAs) are among the ovular factors that may regulate pollen cytosolic Ca 2+ levels (Wu et al, 2010 ; Aloisi et al, 2015 ). PAs are aliphatic polycations linked to a plethora of physiological processes in plants (Del Duca et al, 2000 ; Takahashi and Kakehi, 2010 ; Tiburcio et al, 2014 ; Cai et al, 2015b ). Even if they are found in every plant cell at concentrations ranging from 10 μM to approximately millimolar levels (Bibi et al, 2012 ), reproductive organs were shown to contain the highest amounts of PAs (Kushad and Orvos, 1990 ; Alburquerque et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Spermine Regulates Pollen Tube Growth by Modulating Ca2+-Dependent Actin Organization and Cell Wall Structure

et al. 2017

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Proper growth of the pollen tube depends on an elaborate mechanism that integrates several molecular and cytological sub-processes and ensures a cell shape adapted to the transport of gametes. This growth mechanism is controlled by several molecules among which cytoplasmic and apoplastic polyamines. Spermine (Spm) has been correlated with various physiological processes in pollen, including structuring of the cell wall and modulation of protein (mainly cytoskeletal) assembly. In this work, the effects of Spm on the growth of pear pollen tubes were analyzed. When exogenous Spm (100 μM) was supplied to germinating pollen, it temporarily blocked tube growth, followed by the induction of apical swelling. This reshaping of the pollen tube was maintained also after growth recovery, leading to a 30–40% increase of tube diameter. Apical swelling was also accompanied by a transient increase in cytosolic calcium concentration and alteration of pH values, which were the likely cause for major reorganization of actin filaments and cytoplasmic organelle movement. Morphological alterations of the apical and subapical region also involved changes in the deposition of pectin, cellulose, and callose in the cell wall. Thus, results point to the involvement of Spm in cell wall construction as well as cytoskeleton organization during pear pollen tube growth.

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“…Recently, Torsten and Aaron (2018) hypothesized that mTG used in food preparation could favor celiac disease initiation and progress. As TGs are also present in plants (Del Duca et al, 2000, 2010; Skovbjerg et al, 2002; Serafini-Fracassini et al, 2009), plant-derived food could be another source for TGs that might reach the reach intestinal tract where these TGs (food derived ones and mTG from microbiota) could play a pathogenic role. This last hypothesis is suggestive but, to our knowledge, not supported by solid experimental evidence.…”

Section: Discussionmentioning

confidence: 99%

Immunoreactivity of Gluten-Sensitized Sera Toward Wheat, Rice, Corn, and Amaranth Flour Proteins Treated With Microbial Transglutaminase

et al. 2019

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The aim of this study was to analyze the effects of microbial transglutaminase (mTG) on the immunoreactivity of wheat and gluten-free cereals flours to the sera of patients with celiac disease (CD) and non-celiac gluten sensitivity (NCGS). Both doughs and sourdoughs, the latter prepared by a two-step fermentation with Lactobacillus sanfranciscensis and Candida milleri , were studied. In order to evaluate the IgG-binding capacity toward the proteins of the studied flours, total protein as well as protein fractions enriched in albumins/globulins, prolamins and glutelins, were analyzed by SDS-PAGE and enzyme-linked immunosorbent assay (ELISA). Results showed that while mTG modified both gluten and gluten-free flour by increasing the amount of cross-linked proteins, it did not affect the serum's immune-recognition. In fact, no significant differences were observed in the immunoreactivity of sera from CD and NCGS patients toward wheat and gluten-free protein extracts after enzyme treatment, nor did this biotechnological treatment affect the immunoreactivity of control samples or the sera of healthy patients. These results suggest that mTG may be used as a tool to create innovative gluten and gluten-free products with improved structural properties, without increasing the immune-reactivity toward proteins present either in doughs or in sourdoughs.

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Tuber Vegetative Stages and Cell Cycle in Helianthus tuberosus: Protein Pattern and their Modification by Spermidine

Cited by 23 publications

References 30 publications

Senescence and programmed cell death in plants: polyamine action mediated by transglutaminase

Senescence and programmed cell death in plants: polyamine action mediated by transglutaminase

Spermine Regulates Pollen Tube Growth by Modulating Ca2+-Dependent Actin Organization and Cell Wall Structure

Immunoreactivity of Gluten-Sensitized Sera Toward Wheat, Rice, Corn, and Amaranth Flour Proteins Treated With Microbial Transglutaminase

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