Tuberculosis: Hey There, Lonely Guy!

Zhu, Junhao; Javid, Babak

doi:10.1016/j.molcel.2015.03.004

Cited by 2 publications

(1 citation statement)

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“…In M. tuberculosis the excess of cytokinin breakdown products increased sensitivity to nitric oxide (NO) 15,21–23 . Since BpLOG is a homologous of MtLOG, we tested the susceptibility of Bp to oxidative stress conditions.…”

Section: Resultsmentioning

confidence: 99%

A LONELY GUY protein of Bordetella pertussis with unique features is related to oxidative stress

Moramarco

Pezzicoli

Salvini

et al. 2019

Sci Rep

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The Gram-negative bacterium B. pertussis is the causative agent of whooping cough. This infection is re-emerging and new features related to Bordetella pathogenesis and microbiology could be relevant to defeat it. Therefore, we focused our attention on BP1253, a predicted exported protein from B. pertussis erroneously classified as lysine decarboxylase. We showed that BP1253 shares the highly conserved motif PGGxGTxxE and the key catalytic amino-acid residues with newly structurally characterized “LONELY GUY” (LOG) proteins. Biochemical studies have confirmed that this protein functions as a cytokinin-activating enzyme since it cleaves the N-glycosidic linkage between the base and the ribose, leading to the formation of free bases, which are the active form of plant hormones called cytokinins. Remarkably, BP1253 selectively binds monophosphate nucleotides such as AMP, GMP and CMP, showing a wider variety in binding capacity compared to other LOGs. Cytokinin production studies performed with B. pertussis have revealed 6-O-methylguanine to be the physiological product of BP1253 in agreement with the higher activity of the enzyme towards GMP. 6-O-methylguanine is likely to be responsible for the increased sensitivity of B. pertussis to oxidative stress. Although BP1253 has a primary sequence resembling the hexameric type-II LOGs, the dimeric state and the presence of specific amino-acids suggests that BP1253 can be classified as a novel type-II LOG. The discovery of a LOG along with its product 6-O-methylguanine in the human pathogen B. pertussis may lead to the discovery of unexplored functions of LOGs, broadening their role beyond plants.

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Section: Resultsmentioning

confidence: 99%