Tubular Constructs as Artificial Urinary Conduits

Sloff, Marije; Simaioforidis, Vasileios; Tiemessen, Dorien M.; Janke, Heinz P.; Kortmann, Barbara; Roelofs, L.A.J.; Geutjes, Paul J.; Oosterwijk, Egbert; Feitz, W.F.J.

doi:10.1016/j.juro.2016.04.092

Cited by 16 publications

(15 citation statements)

References 27 publications

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“…Previously, cell seeded constructs have been tested as urostomy constructs, but this was not very successful, although urine exposure of the collagen graft was limited. 20 In an ongoing pilot experiment, we noticed that creation of an artificial conduit with preimplanted COL-Vicryl-EtO tubes was not successful due to disconnection from the ureter and poor integration to the surrounding tissue. In contrast, when the completely remodeled COL-Vicryl-γ construct was used, this ultimately resulted in a functional conduit (results not shown, manuscript in preparation), showing that urine did not influence the outcome of constructs aligned with cells but that the outcome is more dependent on the sterilization method used.…”

Section: Discussionmentioning

confidence: 99%

“…Subcutaneous implantation was performed to form vascularized neo-tissue as previously described. 20 Under general anesthesia, subcutaneous pockets were created on the right flank below the muscle layer of the skin, through a 5 cm incision above the shoulder of the right hind leg. In three animals, one COL-Vicryl-EtO construct was preimplanted, and in three other animals, both the COL-Vicryl-EtO and COL-Vicryl-γ were implanted in different pockets on the same flank.…”

Section: Materials and Methodsmentioning

confidence: 99%

“… 17 − 19 In a previous study, a γ-irradiated hybrid tubular construct from type I collagen and a biodegradable Vicryl polymer mesh was used to create an artificial urinary conduit in a porcine model. 20 Interestingly, a functional conduit with urinary flow could be created after subcutaneous preimplantation, although severe shrinkage of the graft and skin contraction was observed. The implanted material was completely remodeled and did not provide sufficient structural integrity to prevent contraction.…”

Section: Introductionmentioning

confidence: 99%

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The Impact of γ-Irradiation and EtO Degassing on Tissue Remodeling of Collagen-based Hybrid Tubular Templates

Sloff

Janke

Jonge

et al. 2018

ACS Biomater. Sci. Eng.

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Clinical implementation of novel products for tissue engineering and regenerative medicine requires a validated sterilization method. In this study, we investigated the effect of γ-irradiation and EtO degassing on material characteristics in vitro and the effect on template remodeling of hybrid tubular constructs in a large animal model. Hybrid tubular templates were prepared from type I collagen and Vicryl polymers and sterilized by 25 kGray of γ-irradiation or EtO degassing. The in vitro characteristics were extensively studied, including tensile strength analysis and degradation studies. For in vivo evaluation, constructs were subcutaneously implanted in goats for 1 month to form vascularized neo-tissue. Macroscopic and microscopic appearances of the γ- and EtO-sterilized constructs slightly differed due to additional processing required for the COL-Vicryl-EtO constructs. Regardless of the sterilization method, incubation in urine resulted in fast degradation of the Vicryl polymer and decreased strength (<7 days). Incubation in SBF was less invasive, and strength was maintained for at least 14 days. The difference between the two sterilization methods was otherwise limited. In contrast, subcutaneous implantation showed that the effect of sterilization was considerable. A well-vascularized tube was formed in both cases, but the γ-irradiated construct showed an organized architecture of vasculature and was mechanically more comparable to the native ureter. Moreover, the γ-irradiated construct showed advanced tissue remodeling as shown by enhanced ECM production. This study shows that the effect of sterilization on tissue remodeling cannot be predicted by in vitro analyses alone. Thus, validated sterilization methods should be incorporated early in the development of tissue engineered products, and this requires both in vitro and in vivo analyses.

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Section: Discussionmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Impact of γ-Irradiation and EtO Degassing on Tissue Remodeling of Collagen-based Hybrid Tubular Templates

Sloff

Janke

Jonge

et al. 2018

ACS Biomater. Sci. Eng.

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“…This model was selected due to the genetic, anatomical and structural similarities between human and pig muscles [ 34 , 35 ] and based on our observations of the myofibre regeneration process under different conditions in this animal model [ 24 , 25 ]. Furthermore, pigs, particularly piglets, have a good and fast regenerative capacity [ 36 ]. The size, proximity, and accessibility of the left and right longissimus lumborum muscles in pigs were also very important criteria that were considered.…”

Section: Discussionmentioning

confidence: 99%

Simvastatin Impairs the Inflammatory and Repair Phases of the Postinjury Skeletal Muscle Regeneration

Otrocka-Domagała

Paździor-Czapula

Maślanka

2018

BioMed Research International

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Background Recent clinical data have suggested that the chronic use of high-lipophilic statins impairs the regenerative capacity of skeletal muscle. Because this activity of statins is poorly understood, we aimed to investigate the effect of simvastatin (SIM) on postinjury myofibre regeneration. Methods The porcine model was used in this study. The animals were divided into two groups: nontreated (control; n=24) and SIM-treated (40 mg/day; n=24). On the 15th day (day 0) of the experiment, a bupivacaine hydrochloride- (BPVC-) induced muscle injury was established, and the animals were sacrificed in the following days after muscle injury. The degree of regeneration was assessed based on histopathological and immunohistochemical examinations. The presence and degree of extravasation, necrosis, and inflammation in the inflammatory phase were assessed, whereas the repair phase was evaluated based on the numbers of muscle precursor cells (MPCs), myotube and young myofibres. Results In the inflammatory phase, SIM increased the distribution and prolonged the period of extravasation, prolonged the duration of necrosis, and prolonged and enhanced the infiltration of inflammatory cells. In the repair phase, SIM delayed and prolonged the activity of MPCs, delayed myotube formation, and delayed and decreased the formation of young myofibres. Our results indicated that SIM did not improve blood vessel stabilization at the site of the injury, did not exert an anti-inflammatory effect, prolonged and enhanced the inflammatory response, and impaired MPC activity, differentiation, and fusion. Moreover, SIM appeared to reduce M1 macrophage activity, resulting in slower removal of necrotic debris and sustained necrosis. Conclusion This study shows that SIM negatively affects the inflammatory and repair phases of the postinjury muscle regeneration. These findings are unique, strengthen the available knowledge on the side effects of SIM, and provide evidence showing that statin therapy is associated with an increased risk of impairment of the regenerative capacity of muscle.

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“…Despite initial reports which failed to demonstrate the necessity of using a cell based approach, results from studies on induced regeneration of tubular organs like ureter, urethra, or blood vessels indicated better regeneration outcomes within cell seeded constructs . Soon, there will be a chance to receive the final answer which strategy is more efficient and should be translated into clinics.…”

Section: Urinary Conduit Constructionmentioning

confidence: 99%

Concise Review: Tissue Engineering of Urinary Bladder; We Still Have a Long Way to Go?

Adamowicz

Pokrywczyńska

Breda

et al. 2017

Stem Cells Translational Medicine

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Regenerative medicine is a new branch of medicine based on tissue engineering technology. This rapidly developing field of science offers revolutionary treatment strategy aimed at urinary bladder regeneration. Despite many promising announcements of experimental urinary bladder reconstruction, there has been a lack in commercialization of therapies based on current investigations. This is due to numerous obstacles that are slowly being identified and precisely overcome. The goal of this review is to present the current status of research on urinary bladder regeneration and highlight further challenges that need to be gradually addressed. We put an emphasis on expectations of urologists that are awaiting tissue engineering based solutions in clinical practice. This review also presents a detailed characteristic of obstacles on the road to successful urinary bladder regeneration from urological clinician perspective. A defined interdisciplinary approach might help to accelerate planning transitional research tissue engineering focused on urinary tracts. STEM CELLS TRANSLATIONAL MEDICINE 2017;6:2033-2043 SIGNIFICANCE STATEMENTThe goal of this review is to present the current status of research on urinary bladder regeneration and highlight further challenges that need to be gradually addressed. We put an emphasis on expectations of urologists that are awaiting tissue engineering based solutions in clinical practice. This review presents a detailed characteristic of obstacles on the road to successful urinary bladder regeneration from a urological clinician perspective.

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Tubular Constructs as Artificial Urinary Conduits

Cited by 16 publications

References 27 publications

The Impact of γ-Irradiation and EtO Degassing on Tissue Remodeling of Collagen-based Hybrid Tubular Templates

The Impact of γ-Irradiation and EtO Degassing on Tissue Remodeling of Collagen-based Hybrid Tubular Templates

Simvastatin Impairs the Inflammatory and Repair Phases of the Postinjury Skeletal Muscle Regeneration

Concise Review: Tissue Engineering of Urinary Bladder; We Still Have a Long Way to Go?

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