Tubulogenesis

Iruela‐Arispe, M. Luisa; Beitel, Greg J.

doi:10.1242/dev.070680

Cited by 104 publications

(106 citation statements)

References 47 publications

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“…Since fluid movement does not seem to be the main driver of the AP growth, we explored other cellular mechanisms contributing to AP expansion. Cell division is important for lumen growth in different organs 7 . This led us to explore in detail the process of cell division during expansion by high temporal resolution 4D live imaging.…”

Section: Articlementioning

confidence: 99%

“…Lumen formation is initiated either by invagination of the epithelium, by hollowing mechanisms in which opposed apical surfaces are separated by the secretion of extracellular matrix and fluid, or by cavitation in which loss of cells inside the organ generates a new space 7 . The acquisition of apicobasal (ab) polarity seems to be a shared feature in all cavity-forming processes, while other aspects such cell proliferation, secretion of repulsive molecules or cell rearrangements are particular to each cavity 8 .…”

mentioning

confidence: 99%

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Mitotic cell rounding and epithelial thinning regulate lumen growth and shape

et al. 2015

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Many organ functions rely on epithelial cavities with particular shapes. Morphogenetic anomalies in these cavities lead to kidney, brain or inner ear diseases. Despite their relevance, the mechanisms regulating lumen dimensions are poorly understood. Here, we perform live imaging of zebrafish inner ear development and quantitatively analyse the dynamics of lumen growth in 3D. Using genetic, chemical and mechanical interferences, we identify two new morphogenetic mechanisms underlying anisotropic lumen growth. The first mechanism involves thinning of the epithelium as the cells change their shape and lose fluids in concert with expansion of the cavity, suggesting an intra-organ fluid redistribution process. In the second mechanism, revealed by laser microsurgery experiments, mitotic rounding cells apicobasally contract the epithelium and mechanically contribute to expansion of the lumen. Since these mechanisms are axis specific, they not only regulate lumen growth but also the shape of the cavity.

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Section: Articlementioning

confidence: 99%

mentioning

confidence: 99%

Mitotic cell rounding and epithelial thinning regulate lumen growth and shape

et al. 2015

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“…Biological tube formation and maintenance (tubulogenesis) is a conserved process that requires precise cytoskeletal regulation and is essential for organogenesis in all metazoans (Iruela-Arispe and Beitel, 2013). The Caenorhabditis elegans excretory cell (EC) is a single-celled epithelial tube in which the cell's apical domain abuts the lumen and the basolateral domain is the outer edge.…”

Section: Introductionmentioning

confidence: 99%

A network of conserved formins, regulated by the guanine exchange factor EXC-5 and the GTPase CDC-42, modulates tubulogenesis in vivo

Shaye

Greenwald

2016

Development

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The C. elegans excretory cell (EC) is a powerful model for tubulogenesis, a conserved process that requires precise cytoskeletal regulation. EXC-6, an ortholog of the diseaseassociated formin INF2, coordinates cell outgrowth and lumen formation during EC tubulogenesis by regulating F-actin at the tip of the growing canal and the dynamics of basolateral microtubules. EXC-6 functions in parallel with EXC-5/FGD, a predicted activator of the Rho GTPase Cdc42. Here, we identify the parallel pathway: EXC-5 functions through CDC-42 to regulate two other formins: INFT-2, another INF2 ortholog, and CYK-1, the sole ortholog of the mammalian diaphanous (mDia) family of formins. We show that INFT-2 promotes F-actin accumulation in the EC, and that CYK-1 inhibits INFT-2 to regulate F-actin levels and EXC-6-promoted outgrowth. As INF2 and mDia physically interact and cross-regulate in cultured cells, our work indicates that a conserved EXC-5−CDC-42 pathway modulates this regulatory interaction and that it is functionally important in vivo during tubulogenesis.

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“…24 Thus, understanding the genesis of these tubules is essential in developmental biology. Many organs develop from simple tubules that branch and ramify into complex treelike structures, 33,34 favoring an increase in the surface area and exchange of nutrients. A novel role for Cdc42-mediated tubulogenesis during pancreatic development has been established by removing Cdc42 at the onset of pancreatic development.…”

Section: Skin Morphogenesismentioning

confidence: 99%

“…33 Cdc42 has recently been shown to be important for normal podocyte physiology. 38 In mice lacking Cdc42 in podocytes, the foot processes of developing podocytes displayed abnormal junctional complexes.…”

Section: Skin Morphogenesismentioning

confidence: 99%