Tuftelin and HIFs expression in osteogenesis

Bobek, Jan; Oralová, Veronika; Kratochvílová, Adéla; Zvackova, Ivana; Lesot, Hervé; Matalová, Eva

doi:10.1007/s00418-019-01813-4

Cited by 4 publications

(5 citation statements)

References 49 publications

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“…This is confirmed by the observations of Shipeng et al in an invitro fibroblast model where they observed an increase in TUFT1 expression (FC = 2.4) in response to their exposure to low intensity red light [29]. Also in our analysis we found overexpression of TUFT1 2 h after the introduction of the anti-TNF drug into culture, which, in conjunction with previous reports [7,24,28,29] suggests that in fibroblasts under the influence of an external factor, the level of TUFT1 will increase. Interesting, in the context of our work are the observations of Kawasaki et al, who emphasize the possibility of using changes in expression of TUFT1 not only as a complementary molecular marker, but also as a new, attractive therapeutic target.…”

Section: Discussionsupporting

confidence: 93%

“…Research conducted by Bobek et al indicate that the role of tuftelin in particular biological and cellular processes depends on the type of cells [28]. This is confirmed by the observations of Shipeng et al in an invitro fibroblast model where they observed an increase in TUFT1 expression (FC = 2.4) in response to their exposure to low intensity red light [29].…”

Section: Discussionmentioning

confidence: 63%

“…Research conducted by Bobek et al . indicate that the role of tuftelin in particular biological and cellular processes depends on the type of cells [ 28 ]. This is confirmed by the observations of Shipeng et al .…”

Section: Discussionmentioning

confidence: 99%

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Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A

Grabarek¹,

Schweizer²,

Adwent³

et al. 2019

pdia

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Introduction: Adalimumab and cyclosporine A are drugs used in moderate to severe forms of psoriasis. Despite the molecular orientation of the drugs, there is a loss of adequate cell sensitivity to the anti-cytokine therapy. Aim: To determine the changes in the gene expression profile associated with drug resistance in the culture of normal human dermal fibroblasts (NHDF) exposed to adalimumab or cyclosporine A compared to the controls. Material and methods: NHDF was exposed to adalimumab/cyclosporine A for 2, 8, 24 h compared to the control culture. Molecular analysis was performed using mRNA and miRNA microarray techniques. The obtained results were analysed using PL-Grid infrastructure (p < 0.05). Results: Of the 22277 ID mRNA, 47 are associated with drug resistance, of which the change in expression of 17 mRNA ID is statistically significant (p < 0.05). The greatest change in transcriptional activity (FC ≥ 1.3) was observed for GLO1, SLC10A3, TUFT1, STATH, ABCB1, AGTR1. Expression of these genes can be regulated by miR-199a-5p, miR-1231, miR-34a, miR-3188, and miR-106a (except AGTR1). Conclusions: The analysis of changes in the expression of mRNA and miRNA related to drug resistance gives the possibility of monitoring the effectiveness of anti-cytokine therapy.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 63%

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Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A

Grabarek¹,

Schweizer²,

Adwent³

et al. 2019

pdia

View full text Add to dashboard Cite

show abstract

“…Accumulating data have demonstrated that many kinds of abnormal signaling pathways are associated with HCC by interacting with highly complex genetic aberrations such as abnormal methylation, histone modification, and chromosome remodeling[ 28 ]. Recently, TUFT1 has been shown to exist in many tissues and has been identified as a useful biomarker associated with poor prognosis in several cancers[ 29 - 31 ]. However, only limited studied were available on the relationship between TUFT1 and HCC.…”

Section: Discussionmentioning

confidence: 99%

Oncogenic tuftelin 1 as a potential molecular-targeted for inhibiting hepatocellular carcinoma growth

Wu¹,

Zheng²,

Ye³

et al. 2021

WJG

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BACKGROUND Abnormal tuftelin 1 (TUFT1) has been reported in multiple cancers and exhibits oncogenic roles in tumor progression. However, limited data are available on the relationship between TUFT1 and hepatocellular carcinoma (HCC), and the exact biological mechanism of TUFT1 is still poorly understood in HCC. AIM To investigate TUFT1 expression in HCC and how interfering TUFT1 transcription affects HCC growth. METHODS TUFT1 in HCC and non-HCC tissues based on databases of the Cancer Genome Atlas and Oncomine were analyzed, and TUFT1 in human HCC tissues on microarray were detected by immunohistochemistry for clinicopathological features, overall survival, and disease-free survival. HCC cells were transfected with constructed vectors of TUFT1 that interfere or over-express TUFT1 for analyzing the biological behaviors of HCC cells. Proliferation, invasion, migration, and apoptosis of cells were detected by cell counting kit-8, scratch assay, transwell tests, and flow cytometry and confirmed by Western blotting, respectively. RESULTS Abnormal TUFT1 levels in databases expressed in HCC at messenger RNA (mRNA) level and HCC tissues were mainly located in cytoplasm and membrane. The level of TUFT1 expression in the HCC group was significantly higher ( χ 2 = 18.563, P < 0.001) than that in the non-cancerous group, closely related to clinical staging, size, vascular invasion of tumor, hepatitis B e-antigen positive, and ascites ( P < 0.01) of HCC patients, and negatively to HCC patients’ overall survival and disease-free survival ( P < 0.001). After interfering with TUFT1 transcription at mRNA level in the MHCC-97H cells by the specific TUFT1-short hairpin RNA, cell proliferation, invasion, and metastasis were significantly inhibited with increasing apoptosis rate. In contrast, proliferation, invasion, and migration were significantly enhanced after over-expression of TUFT1 mRNA in Hep3B cells in vitro . CONCLUSION Oncogenic TUFT1 was associated with the progression of HCC and could be a potential molecular-target for inhibiting HCC growth.

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“…[ 39 ] confirmed that Sr 2+ enhances VEGF and Ang-1 expressions. Hypoxia-inducible factors (HIFs) are transcriptional complexes that are heterodimers consisting of HIF-α and HIF-β subunits [ 92 ]. The hypoxia-inducible factor 1-alpha (HIF-1α) regulates VEGF's transcriptional activation of hypoxia and insulin-like growth factor-I (IGF-I) to stimulate vascular growth [ 93 , 94 ].…”

Section: Mechanisms Of Sr At Cellular and Molecular Levelsmentioning

confidence: 99%

Advanced applications of strontium-containing biomaterials in bone tissue engineering

Sheng¹,

Li²,

Wang³

et al. 2023

Materials Today Bio

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Tuftelin and HIFs expression in osteogenesis

Cited by 4 publications

References 49 publications

Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A

Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A

Oncogenic tuftelin 1 as a potential molecular-targeted for inhibiting hepatocellular carcinoma growth

Advanced applications of strontium-containing biomaterials in bone tissue engineering

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