Tumor angiogenesis in human lung adenocarcinoma

Yamazaki, Kohichi; Takekawa, Hironori; Sukoh, Noriaki; Watanabe, Naomi; Ogura, Shinji; Nakajima, Isao; Isobe, Hiroshi; Kawakami, Yoshikazu; Abe, Shosaku; Inoue, Kazuaki

doi:10.1002/1097-0142(19941015)74:8<2245::aid-cncr2820740807>3.0.co;2-x

Cited by 151 publications

(80 citation statements)

References 20 publications

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“…Microvessel density has been shown to be an independent parameter of the severity of tumour disease and a predictor of prognosis of breast cancer patients in most but not all studies addressing this issue (Weidner et al, 1991(Weidner et al, , 1992Bosari et al, 1992;Horak et al, 1992;Toi et al, 1993, Axelsson et al, 1995. A similar correlation has also been demonstrated with other human malignancies, including head and neck squamous cell carcinoma (Gasparini et al, 1993), lung adenocarcinoma (Yamazaki et al, 1994), rectal carcinoma (Saclarides et al, 1994), testicular germ cell tumours (Olivarez et al, 1994), prostate cancer , tumours of the oral cavity (Williams et al, 1994) and gastric carcinoma (Maeda et al, 1995 rie receptor in breast cancer P Salven et al higher when staining was performed using the clone 7E8 antibody (Table I). No specific staining was observed when the sections were incubated with antigen-blocked antibody instead of the primary antibodies, or with the peroxidaseconjugated secondary antibody only.…”

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confidence: 78%

Endothelial Tie growth factor receptor provides antigenic marker for assessment of breast cancer angiogenesis

Salvén

Joensuu²,

Heikkilä³

et al. 1996

Br J Cancer

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Summary Breast cancer prognosis has previously been linked to the degree of tumour vascularisation. In order to establish additional markers for tumour angiogenesis, we have used monoclonal antibodies against the endothelial Tie receptor tyrosine kinase to study the degree of vascularisation of breast carcinomas and the regulation of Tie expression in the vascular endothelial cells. Antibodies were used for Tie detection and the results were correlated with other prognostic markers. Of four monoclonal antibodies directed against different epitopes of the Tie extracellular domain, two reacted against Tie in unfixed histopathological sections of breast carcinomas. One of these antibodies (clone 7e8) was specific for the endothelial cells whereas the other (clone 10fl 1) also reacted with basement membranes and occasional carcinoma cells. When Tie expression was studied with the antibody clone 7e8, all 27 carcinomas, two in situ carcinomas, samples of histologically normal breast tissue (n = 16) or normal skin or lymph node tissue (n= 5) showed staining. Microvessel counts were higher in carcinomas (median 14; range 3 -27) than in fibrodenomas (median 10; range 5 -18) or histologically normal breast tissue (median 7; range 3 -15, P = 0.0006). A similar result was obtained using antibodies against the CD31 (PECAM) antigen. Microvessel counts in 7e8 staining were not significantly associated with primary tumour size, axillary nodal status, histological grade or staining for oestrogen receptor, progesterone receptor, Ki-67 proliferation marker or p53 oncoprotein.

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mentioning

confidence: 78%

Endothelial Tie growth factor receptor provides antigenic marker for assessment of breast cancer angiogenesis

Salvén

Joensuu²,

Heikkilä³

et al. 1996

Br J Cancer

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“…Generally speaking, neovascularity assessed by the microvessel counting in the tumour tissue has a prognostic value in a variety of cancers (Yamazaki et al, 1994;Maeda et al, 1995;Brawer, 1996;Gasparini et al, 1996;Takabayashi et al, 1996). In addition, the density of microvessels was not correlated with the tissue VEGF concentration measured by ELISA in soft tissue sarcomas.…”

Section: Discussionmentioning

confidence: 98%

“…Several reports have demonstrated a positive correlation between an increased number of new vessels and the development of metastasis (Gimbrone et al, 1974;Liotta et al, 1974;Liotta et al, 1991). It has been reported that neovasularity assessed by intratumoral microvessel density (MVD) correlates with clinicopathologic factors and the prognosis of a variety of cancers (Yamazaki et al, 1994;Maeda et al, 1995;Brawer, 1996;Gasparini et al, 1996;Takabayashi et al, 1996).…”

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confidence: 99%

Concentration of vascular endothelial growth factor in the tumour tissue as a prognostic factor of soft tissue sarcomas

et al. 2001

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Previous studies have shown that the prognosis of patients who have tumours with high microvessel density (MVD) is worse than that of patients who have a lower density in a variety of cancers. In this study, we investigated the clinical relevance of neovascularity assessed by MVD and the concentration of vascular endothelial growth factor (VEGF) in the tumour tissue of patients with soft tissue sarcoma in comparison with major clinicohistologic parameters by univariate and multivariate analysis. In 115 patients with soft tissue sarcoma, MVD was measured by counting vessels stained with factor VIII antibody. The concentration of VEGF in the tumour tissue was determined by enzyme-linked immunosorbent assay. These parameters were then compared with disease outcome. The concentration of VEGF in the tumour tissue, but not MVD, was found to be correlated with disease outcome in patients with soft tissue, sarcoma. VEGF concentration in the tumour tissue showed a relationship with the clinical stage and histologic grade of the tumour. There was no significant difference in the levels of tissue VEGF concentration and MVD among soft tissue sarcomas classified according to histologic type. The level of tissue VEGF concentration in patients who had subsequent local recurrence and metastasis were significantly higher than the respective values in patients who did not have such disease outcome. No significant correlation existed between MVD and the concentration of VEGF in the tumour tissue. Univariate analysis showed that a high tissue VEGF concentration was associated with poor overall survival of the patient and a greater probability that local recurrence and metastasis had occurred. Multivariate analysis revealed that the tissue concentration of VEGF is an independent prognostic factor for the disease outcome of patients with soft tissue sarcoma. VEGF concentration in the tumour tissue, but not MVD, is an additional prognostic parameter for disease outcome in patients with soft tissue sarcoma, regardless of histologic type. © 2001 Cancer Research Campaign http://www.bjcancer.com

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“…In the 14 remaining eligible studies (Macchiarini et al, 1994;Yamazaki et al, 1994;Fontanini et al, 1995;Mattern et al, 1995;Chandrachud et al, 1997;Giatromanolaki et al, 1997;Takanami et al, 1997;Duarte et al, 1998;Imoto et al, 1998;Aikawa et al, 1999;D'Amico et al, 1999;Ohta et al, 1999;Sheng et al, 2000;Yano et al, 2000), published between 1994 and 2000, the total number of patients was 1866 ranging from 28 to 408. The main characteristics of these 14 studies are shown in Table 1.…”

Section: Studies Selection and Characteristicsmentioning

confidence: 99%

The role of microvessel density on the survival of patients with lung cancer: a systematic review of the literature with meta-analysis

et al. 2002

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In order to determine whether angiogenesis is a prognostic marker in lung cancer, we performed a systematic review of the literature to assess the prognostic value on survival of microvessel count in patients with lung cancer. Published studies were identified by an electronic search in order to aggregate survival results, after a methodological assessment using a quality scale designed by the European Lung Cancer Working Party. To be eligible, a study had to deal with microvessel count assessment in lung cancer patients on the primary site and to provide survival analysis according to microvessel count expression. Microvessel count has been assessed on surgical samples by immunohistochemistry using factor VIII in 14 studies, CD34 in 10 and CD31 in eight. Respectively 1866, 1440 and 1093 non-small cell lung cancer patients were considered. The overall median quality scores were respectively 52, 59 and 59% for studies assessing microvessel count via factor VIII, CD34 and CD31, without significant difference between studies evaluable or not for meta-analysis nor between studies with significant or non significant results. Seven 'factor VIII' studies, nine 'CD34' and seven 'CD31' provided sufficient data allowing a metaanalysis on survival and were evaluable for results aggregation. This showed that a high microvessel count in the primitive lung tumour was a statistically significant poor prognostic factor for survival in non small cell lung cancer whatever it was assessed by factor VIII (HR: 1.81; 95% CI: 1.16 -2.84), CD34 (HR: 1.99; 95% CI: 1.53 -2.58) or CD31 (HR: 1.80; 95% CI: 1.10 -2.96). Variations in survival among the individual studies can be explained in addition to patients selection criteria by the heterogeneous methodologies used to stain and count microvessels: different antibody clones, identification of 'hotspots', Weidner or Chalkey counting method, cut-off selection. Microvessel count, reflecting the angiogenesis, appears to be a poor prognostic factor for survival in surgically treated non small cell lung cancer but standardisation of angiogenesis assessment by the microvessel count is necessary.

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Tumor angiogenesis in human lung adenocarcinoma

Cited by 151 publications

References 20 publications

Endothelial Tie growth factor receptor provides antigenic marker for assessment of breast cancer angiogenesis

Endothelial Tie growth factor receptor provides antigenic marker for assessment of breast cancer angiogenesis

Concentration of vascular endothelial growth factor in the tumour tissue as a prognostic factor of soft tissue sarcomas

The role of microvessel density on the survival of patients with lung cancer: a systematic review of the literature with meta-analysis

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