Tumor fluid thromboplastin activity

Frank, Allen; Ed, Holyoke

doi:10.1002/ijc.2910030516

Cited by 22 publications

(6 citation statements)

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“…The presence of increased thromboplastin activity in the peripheral fluids of the Lewis Sarcoma T-241 in vivo, over comparable " normal " tissue fluids, has been demonstrated (Frank and Holyoke, 1968). The present experiments made in tissue culture also demonstrate thromboplastin activity released by the T-241 Lewis Sarcoma cells into their environments.…”

Section: Discussionsupporting

confidence: 74%

“…Thornes (1961), demonstrated that incubation of tumor slices in saline resulted in the release of thromboplastin activity into the medium. Using the Lewis Sarcoma, T-241, Frank and Holyoke (1968) demonstrated by means of small millipore collection chambers in vivo, that tissue fluid samples collected from tumor periphery showed more thromboplastin activity than samples from normal subcutaneous tissues.…”

mentioning

confidence: 99%

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Tumor thromboplastin activity In vitro

Frank

Weiss

1972

Intl Journal of Cancer

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The thromboplastin activity of tissue culture media, before and afrer incubation with T-241 Lewis sarcoma cells of mouse origin, or with embryonic mouse epithelial or muscle fibroblasts, was mcasured by means of a calcium reconstitution assay. Incubation of the embryonic epithelial or muscle fibroblasts increased the thromboplastin activity of the tissue culture media more than did incubation with tumor cells, even though analysis of protein showed that there were more tumor cells than normal cells present at the time of incubation, It is concluded that there is no evidence for a unique '' Cancer Coagulative Factor '' at least in this system. In vitro studies of the effect of trauma in increasing the release of thromboplastin activity from both normal and tumor tissues, suggest that the mutual destruction of both tissues could account for the elevated levels observed in some patients with cancer.

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Section: Discussionsupporting

confidence: 74%

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confidence: 99%

Tumor thromboplastin activity In vitro

Frank

Weiss

1972

Intl Journal of Cancer

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“…There is an extensive literature describing procoagulant activities (PCAs) of various types associated with disrupted tumor cells (3,12,(91)(92)(93)(94)(95)(96)(97)(98)(99)(100)(101)(102). However, the biological significance of this finding is, in our view, open to serious question: it is unclear how much of the PCA expressed by broken cells in vitro would be expressed by living tumor cells in vivo; also extracts of normal as well as malignant cells may exhibit PCA (103)(104)(105).…”

Section: Tumor Procoagulantsmentioning

confidence: 99%

Fibrin as a component of the tumor stroma: origins and biological significance

1983

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An association between cancer and the coagulation system was suggested by Trousseau more than a century ago and initial reports of fibrin deposition in the stroma of solid tumors date back some 25 years. However, the validity and generality of these observations have only quite recently been established, and their implications for an understanding of tumor biology, metastasis, and therapy are only now coming to be appreciated by investigators in the mainstream of cancer research. This article reviews the current status of fibrin's role in the biology of tumor growth, considering in turn: (1) the evidence that fibrin is present in tumors, the nature of such fibrin, and its relation to plasma fibronectin; (2) the mechanisms by which fibrin may come to be deposited in tumors; and (3) the potential biological and medical significance of tumorassociated fibrin deposition and degradation. Among the last are such important possibilities as a barrier function to the immune response and possible roles in angiogenesis, desmoplasia, and metastasis.

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“…The specific activities of PCA1o and of several plasma membrane-derived enzymes-5'-nucleotidase, ouabain-sensitive Na+-K+-adenosinetriphosphatase, and phosphodiesterase I-were much greater in ultracentrifuge pellets of culture supernatants than in line 10 cell homogenates (10,70,11, and 122 times greater, respectively) or cell-free culture supematants (4, > 315, 6, and 7 times greater, respectively). Enzyme markers for endoplasmic reticulum (NADH oxidoreductase) and lysosomes (,-D-glucuronidase) were only detected at low levels in culture fluids and remained in the l00,OOOg supernatant.…”

mentioning

confidence: 90%